DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Magi and Magi3

DIOPT Version :9

Sequence 1:	NP_001286661.1	Gene:	Magi / 37403	FlyBaseID:	FBgn0034590	Length:	1202	Species:	Drosophila melanogaster
Sequence 2:	NP_001152826.1	Gene:	Magi3 / 99470	MGIID:	1923484	Length:	1476	Species:	Mus musculus

Alignment Length:	1125	Identity:	322/1125 - (28%)
Similarity:	456/1125 - (40%)	Gaps:	387/1125 - (34%)

- Green bases have known domain annotations that are detailed below.

  Fly   158 EKSGGLAPPG---GHHLRPPPPPA-----LKKSASAVI-----GNSASREDVASLSGTSSLNSQM 209
            |:||.|...|   |:....|.|||     .......|:     ...:.|:...|:|....::|.:
Mouse   174 EESGALLESGTYDGNFYGTPKPPAEPSPFQPDPVDQVLFDNEFDTESQRKRTTSVSKMERMDSSL 238

  Fly   210 SVHNQFISGLPSNGIGSNGVHGGAATGRGGPPNSILKGSKENLLQNTYYNGETGDSSLGSECGVG 274
            ....:.......||.||                          ::....:.||      |:|.:.
Mouse   239 PEEEEDEDKEAVNGSGS--------------------------METREMHSET------SDCWMK 271

  Fly   275 DV--QHQNHESHDDPG-----DGLGPLPPKWETAYTERGELYFIDHNTGTSHWLDPRLSKYQKKS 332
            .|  .:|.:.|.|...     :.|.|||..||.|||:.|.:|||||||.|:.||||||.| :.|:
Mouse   272 TVPSYNQTNSSMDFRNYMMRDENLEPLPKNWEMAYTDTGMIYFIDHNTKTTTWLDPRLCK-KAKA 335

  Fly   333 LEDCCEDELPYGWEKIEDSMYGMYFIDHVNRRTQYENPVLEAKRRAAEQSQQQQQMQQQQQQMQE 397
            .|||.:.|||||||||||..||.|::||:|::||:||||.||||:            :|..|.:.
Mouse   336 PEDCEDGELPYGWEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRK------------KQLGQAEI 388

  Fly   398 QRSKTPTALSETMPTEAAEAHEQEEDESPMKLPYKFTRNPAELQGQRINTTLLKSSRGLGFTIVG 462
            ..:|          |:...||              |||:|::|:|..:..:|.||:.|.||||:|
Mouse   389 HSAK----------TDVERAH--------------FTRDPSQLKGVLVRASLKKSTMGFGFTIIG 429

  Fly   463 SDGSAGGDVEEFLQIKTVVPNGPAWLDGQLQTGDVLVYVNDTCVLGYTHHDMVNIFQSILPGERA 527
            .|..     :||||:|.|:.:|||..||::..|||:|.:|..||||:||.|:|.:||.:...:..
Mouse   430 GDRP-----DEFLQVKNVLKDGPAAQDGKIAPGDVIVDINGNCVLGHTHADVVQMFQLVPVNQYV 489

  Fly   528 ALEVCRGYPLPFDPNDPNTEVV-TTMAVDGR----------------------ESDKQRRLNMDG 569
            .|.:|||||||.|..||..::| .|..::|:                      ::.|..::....
Mouse   490 NLTLCRGYPLPDDSEDPVVDIVAATPVINGQSLTKGETCMNTQDFKLGAMVLDQNGKSGQILASD 554

  Fly   570 NYNFLDLSGEGAKKTSGSGSGFILMKKPEIYTFSIMKGSMGFGFTIADSACGQIVKKILDRNCCT 634
            ..|....|.|.....:.|||     .:||:.|..::||..||||.||||..||.||.|||...|.
Mouse   555 RLNGPSESSEQRASLASSGS-----SQPELVTIPLIKGPKGFGFAIADSPTGQKVKMILDSQWCQ 614

  Fly   635 QLMEGDVLLEINGLNVRNKPHFYVVELLKECSQTTPTAVKIQRTPPDPPANNTLAQLNQVGNVAK 699
            .|.:||::.||...||:|..|..|||:||:
Mouse   615 GLQKGDIIKEIYHQNVQNLTHLQVVEVLKQ----------------------------------- 644

  Fly   700 LRKNFVGSGLFRSKTPTADLYSTQVKEVLPMRPKTPLVDTRRSRVQIQSPNNEVDDEGDGAAAAE 764
                                        .|:....||:..|                  |...:.
Mouse   645 ----------------------------FPVGADVPLLILR------------------GGPCSP 663

  Fly   765 RKPLQLQTQGKSNS-SLQELDDIPYMDPYPKISRLSERLAEVTLQGDANGGIYGMPPSMQPLPLP 828
            .|..:.:|..|.|| ||:.::     :|.|                             ||:|.|
Mouse   664 TKTAKTKTDTKENSGSLETIN-----EPIP-----------------------------QPMPFP 694

  Fly   829 LAHHESCYCYDCQAQRYRPGYFVQAQQAAMSGTTGQYSPLQTAHLQNERIQRRVNELLSDRRRVG 893
                                     .....||     ||    .|....:..:...|..|:
Mouse   695 -------------------------PSIIRSG-----SP----KLDPSEVYLKSKTLYEDK---- 721

  Fly   894 FANLDPPQQMQHSPSWRNGALLDVSEDADQCELTEVTLERQALGFGFRIVGGTEEGSQVTVGHIV 958
                 ||.                ::|.|      |.|.:|..|||||::||......:.:|.|:
Mouse   722 -----PPN----------------TKDLD------VFLRKQESGFGFRVLGGDGPDQSIYIGAII 759

  Fly   959 PGGAADQDQRINTGDEILSIDGINVLNSSHHKVVSLVGESALRGQVTMILRRR------------ 1011
            |.|||::|.|:...||::.||||.|...||.:|:.|:..:|..|.|.:.:||:
Mouse   760 PLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHVLLTVRRKIFYGEKQPEDES 824

  Fly  1012 -------RTPLLQQAPVSTQLRRYP---YDVIVSRHENEGFGFVIISSSN--------HYYGSTI 1058
                   .:|.|.:|.:.|  |..|   |||.:.|.|||||||||::|.:        |    .|
Mouse   825 HQAFSQNGSPRLNRAELPT--RSAPQEAYDVTLQRKENEGFGFVILTSKSKPPPGVIPH----KI 883

  Fly  1059 GKLIPGSPADRCGELKVGDRIVAVNRIEIAGMSHGDVVNLIKESGLHVRLTIGVPLKEGGPSPGG 1123
            |::|.||||||||.|||||.|.|||...|..:||.::|.|||::|:.|.||:....:..||..|.
Mouse   884 GRVIDGSPADRCGGLKVGDHISAVNGQSIVDLSHDNIVQLIKDAGVTVTLTVVAEEEHHGPPSGT 948

  Fly  1124 SSVGVSSNTPLQASPSLLKAQLSHQQQLPQQH--LQQAQLNH----------------------- 1163
            :|.        :.||:|             ||  :.|||.||
Mouse   949 NSA--------RQSPAL-------------QHRPMGQAQANHIPGDRIALEGEIGRDVCSSYRHS 992

  Fly  1164 --HHQHLEMPMSMPVPGHGS 1181
              .|:||..|.:..:...||
Mouse   993 WSDHKHLAQPDTAVISVVGS 1012

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Magi	NP_001286661.1	WW	294..323	CDD:278809	18/28 (64%)
		WW	342..372	CDD:238122	20/29 (69%)
		PDZ	443..534	CDD:214570	38/90 (42%)
		DegQ	<455..534	CDD:223343	35/78 (45%)
		PDZ_signaling	606..668	CDD:238492	32/61 (52%)
		PDZ	925..1011	CDD:214570	33/85 (39%)
		PDZ_signaling	1029..1110	CDD:238492	46/91 (51%)
Magi3	NP_001152826.1	Interaction with ADRB1 and TGFA. /evidence=ECO:0000269\|PubMed:15652357	18..108
		PDZ_signaling	24..103	CDD:238492
		NK	125..>199	CDD:302627	10/24 (42%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	184..266		16/113 (14%)
		WW	297..329	CDD:197736	21/31 (68%)
		WW	345..375	CDD:238122	20/29 (69%)
		Interaction with PTEN. /evidence=ECO:0000250	413..495		37/86 (43%)
		PDZ	416..496	CDD:214570	38/84 (45%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	551..575		6/28 (21%)
		PDZ	578..659	CDD:214570	38/161 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	664..691		10/60 (17%)
		PDZ	729..812	CDD:214570	34/88 (39%)
		Interaction with ADGRB1. /evidence=ECO:0000250	729..811		33/87 (38%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	818..844		4/27 (15%)
		PDZ_signaling	851..936	CDD:238492	46/88 (52%)
		Interaction with LPAR2 and GRIN2B. /evidence=ECO:0000250	852..939		45/90 (50%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..966		10/47 (21%)
		PDZ_signaling	1023..1101	CDD:238492
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1109..1151
		MIP-T3	<1166..>1431	CDD:287245
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1168..1476
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	74	1.000	Domainoid score	I9119
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	510	1.000	Inparanoid score	I1307
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000831
OrthoInspector	1	1.000	-	-		otm42887
orthoMCL	1	0.900	-	-		OOG6_102187
Panther	1	1.100	-	-	O	PTHR10316
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3056
SonicParanoid	1	1.000	-	-		X573
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	12	11.850