DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment rig and GEMIN5

DIOPT Version :9

Sequence 1:	NP_611499.3	Gene:	rig / 37335	FlyBaseID:	FBgn0250850	Length:	1235	Species:	Drosophila melanogaster
Sequence 2:	NP_056280.2	Gene:	GEMIN5 / 25929	HGNCID:	20043	Length:	1508	Species:	Homo sapiens

Alignment Length:	1341	Identity:	264/1341 - (19%)
Similarity:	447/1341 - (33%)	Gaps:	483/1341 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 PTPPGNVSLAAAAPDGGLL-YAGIRCINYISAPPANGEQP---------QVVTMSTRINILALDV 65
            |:|....:..:.|..|||. :|....:..:...|..||.|         ::|..:.|::......
Human    10 PSPNWYCARCSDAVPGGLFGFAARTSVFLVRVGPGAGESPGTPPFRVIGELVGHTERVSGFTFSH 74

  Fly    66 SPMWGLGNGGPTKPFAIVGDDLSVQVWDCALGEAVIGHKAHQHQHEARDVRVVHHTTNSVLMSYL 130
            .|       |.....|...||.:|::||......|..|..||            ||.:::..|..
Human    75 HP-------GQYNLCATSSDDGTVKIWDVETKTVVTEHALHQ------------HTISTLHWSPR 120

  Fly   131 ANGNILSMDASDLVIYCVASNTYCRRSTFISPRNHQLTMVRCSPYNDNLFAVGTAMGNVLVCDL- 194
            ....|:|.|... |::|...|....:..||.||.  :..:.|||::::|.|:|...|.|::.|:
Human   121 VKDLIVSGDEKG-VVFCYWFNRNDSQHLFIEPRT--IFCLTCSPHHEDLVAIGYKDGIVVIIDIS 182

  Fly   195 RKMNIVYKFHGHKAPICGLAWREVPAAEDEKTNNLALSAEEWRSRNGGQ-EEKPKTKPPPL-TKS 257
            :|..::::..||...|..:||..:| .||..:.|...::||....||.. .:.|.||...| |.|
Human   183 KKGEVIHRLRGHDDEIHSIAWCPLP-GEDCLSINQEETSEEAEITNGNAVAQAPVTKGCYLATGS 246

  Fly   258 KAAESDDPFDIYNFDH------LEYEFGAPIAERRRKSSEDCGGEFVGLEKPAGAAVLDFVEACE 316
            |    |....|::...      |:    .|..:||       ||   |::              .
Human   247 K----DQTIRIWSCSRGRGVMILK----LPFLKRR-------GG---GID--------------P 279

  Fly   317 SVKAELLASRQEDKTQHVEVTLH--DCEPTKPTGPLSDASTISNKNDASDSTEGSLEVIQYSSSS 379
            :||..|.            :|||  ..:||:
Human   280 TVKERLW------------LTLHWPSNQPTQ---------------------------------- 298

  Fly   380 DDAVIVDGEAAKPKREVLHHIYHQAEVHASGTPQTKSEPQSNLQVVPAISAETISLTSVNSTHLE 444

Human   299 ----------------------------------------------------------------- 298

  Fly   445 TLLVSIDGDEVMMIWNTN----------TGAHAGKNYSKSKTAGKLNNVYWLNNHVIVSLSRHQL 499
             |:.|..|.|::. |:..          :.:..|:|:|:                ::.:|...|
Human   299 -LVSSCFGGELLQ-WDLTQSWRRKYTLFSASSEGQNHSR----------------IVFNLCPLQ- 344

  Fly   500 FFWSVEFERKMLRYKISKDKSHSCHLQDIVSFACDSSKEMIWLCRNNRQIGMMNPKTGRMADFYG 564
                .|.::::| ...|.|:...|  .||.:..|.      |..          |..|..|
Human   345 ----TEDDKQLL-LSTSMDRDVKC--WDIATLECS------WTL----------PSLGGFA---- 382

  Fly   565 TVAFGVRAMAECPDDMNKIALGCSDRRVAFFDISKLTTSCLPIDSVY--------VSSNVYCLAW 621
                  .::|....|:..:|:|..|..:..:       :.|.|.:.|        |.|.|..|.|
Human   383 ------YSLAFSSVDIGSLAIGVGDGMIRVW-------NTLSIKNNYDVKNFWQGVKSKVTALCW 434

  Fly   622 SP---NCLELAFGTFDGTVGILDVERMKVKTHLRTPHKKEVYSLVW---------------QDHF 668
            .|   .|  |||||.||.||:.|....|......|.|||.||:|.|               ....
Human   435 HPTKEGC--LAFGTDDGKVGLYDTYSNKPPQISSTYHKKTVYTLAWGPPVPPMSLGGEGDRPSLA 497

  Fly   669 IY--------------------FIVNRVLGFFDLRKSKIEPTIVNCISRPSYLSIR----DSFLF 709
            :|                    |.:|:::...:..|.|:          |.:..|.    ...:.
Human   498 LYSCGGEGIVLQHNPWKLSGEAFDINKLIRDTNSIKYKL----------PVHTEISWKADGKIMA 552

  Fly   710 VGTDDGLLQIHERDSGMEKSWSPFIRQSALFARY---VTDIAW------CPLDSNKFAVSGNDRS 765
            :|.:||.::|.:         .|.::......::   |..|:|      .|..|...|...|:..
Human   553 LGNEDGSIEIFQ---------IPNLKLICTIQQHHKLVNTISWHHEHGSQPELSYLMASGSNNAV 608

  Fly   766 VYVMEFQPTERNWKTL---------------HTFTANTEKASITSMRWSHTQKHLLLTFHIEGKV 815
            :||       .|.||:               .|.:.:|  |.|||:.||......|::...:|..
Human   609 IYV-------HNLKTVIESSPESPVTITEPYRTLSGHT--AKITSVAWSPHHDGRLVSASYDGTA 664

  Fly   816 CLWNCNAPEKPPLTITYH-----CPMWCGMFLPTNENII------MCSGKALSVELIDIKDALEG 869
            .:|:. ..|:|......|     |..|.    |.:.:.|      .|..|.|: .:.|.....:|
Human   665 QVWDA-LREEPLCNFRGHRGRLLCVAWS----PLDPDCIYSGADDFCVHKWLT-SMQDHSRPPQG 723

  Fly   870 DEKSICPKVDALLNVKWASKSLTQPYAPVLTAAEKKRQRRDQRKAAAKLEVDVANKDQKKIQESV 934
             :|||          :...|.|:||      .|:.|::::...:...||| .:...:::.::|:
Human   724 -KKSI----------ELEKKRLSQP------KAKPKKKKKPTLRTPVKLE-SIDGNEEESMKEN- 769

  Fly   935 TAVIDNPTNDKCTQETPVEEMLE---ALSLDKEQNNRSAKECPKCKEQSPDSFTHSRTCLYLTQK 996
            :..::|..:|:..:|...|..|.   |.::.:|         |.........|
Human   770 SGPVENGVSDQEGEEQAREPELPCGLAPAVSRE---------PVICTPVSSGF------------ 813

  Fly   997 ELNKSALEKLAIVLTEDSAKIDKSVLISKLFSSKVMAKELIATELTNLKHSNTKDIAPLCLAMST 1061
            |.:|..:....|:|.::..|.....||.|.     .|:.|:... |:|.|.:.:::...||.::|
Human   814 EKSKVTINNKVILLKKEPPKEKPETLIKKR-----KARSLLPLS-TSLDHRSKEELHQDCLVLAT 872

  Fly  1062 FKLREELEQ-------------------------------HIAN--------------------- 1074
            .|...||.:                               |:.|
Human   873 AKHSRELNEDVSADVEERFHLGLFTDRATLYRMIDIEGKGHLENGHPELFHQLMLWKGDLKGVLQ 937

  Fly  1075 -----KTLTERHVSLAPSVSFTLWQDCCRAYAKQMEEKGYIMHAATYLFSQGMQSEAIKLFLANE 1134
                 ..||:..|::||:..:.:|.....|:|||:..:...:.||::|.|.....||::|..:|.
Human   938 TAAERGELTDNLVAMAPAAGYHVWLWAVEAFAKQLCFQDQYVKAASHLLSIHKVYEAVELLKSNH 1002

  Fly  1135 YYKEALVHARICLPATDPLIKTVINNWLEHLEGTGNFAAAA 1175
            :|:||:..|:..|...||::|.:..:|...||..|::|.||
Human  1003 FYREAIAIAKARLRPEDPVLKDLYLSWGTVLERDGHYAVAA 1043

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
rig	NP_611499.3	WD40	<80..>215	CDD:295369	36/135 (27%)
		WD40	80..>215	CDD:225201	36/135 (27%)
		WD40 repeat	116..160	CDD:293791	9/43 (21%)
		WD40 repeat	167..204	CDD:293791	10/37 (27%)
		WD40	434..821	CDD:225201	91/470 (19%)
		WD40	446..719	CDD:295369	66/332 (20%)
		WD40	613..874	CDD:295369	73/337 (22%)
		WD40 repeat	617..654	CDD:293791	15/39 (38%)
		WD40 repeat	659..691	CDD:293791	9/66 (14%)
		WD40 repeat	693..732	CDD:293791	6/42 (14%)
		WD40 repeat	744..785	CDD:293791	13/61 (21%)
		WD40 repeat	793..831	CDD:293791	10/37 (27%)
		WD40 repeat	838..861	CDD:293791	5/28 (18%)
GEMIN5	NP_056280.2	Important for interaction with U1 snRNA. /evidence=ECO:0000269\|PubMed:19377484	1..124		28/132 (21%)
		Interaction with U4 snRNA. /evidence=ECO:0000269\|PubMed:27834343, ECO:0000269\|PubMed:27881600, ECO:0000269\|PubMed:27881601, ECO:0007744\|PDB:5GXH, ECO:0007744\|PDB:5GXI, ECO:0007744\|PDB:5H1K, ECO:0007744\|PDB:5H1L	13..15		0/1 (0%)
		WD40	<53..411	CDD:225201	101/582 (17%)
		WD40	57..408	CDD:295369	101/575 (18%)
		WD40 repeat	68..107	CDD:293791	10/45 (22%)
		WD 2	107..148		11/53 (21%)
		WD40 repeat	112..149	CDD:293791	7/37 (19%)
		WD 3	150..189		12/40 (30%)
		WD40 repeat	154..193	CDD:293791	10/38 (26%)
		WD 4	193..264		22/75 (29%)
		WD40 repeat	228..279	CDD:293791	17/82 (21%)
		WD40	240..731	CDD:225201	127/739 (17%)
		WD 5	280..321		13/153 (8%)
		WD40 repeat	288..325	CDD:293791	10/137 (7%)
		WD40 repeat	331..377	CDD:293791	13/85 (15%)
		WD 6	333..374		11/70 (16%)
		WD40	343..668	CDD:295369	81/395 (21%)
		WD 7	377..417		9/56 (16%)
		WD40 repeat	382..422	CDD:293791	9/56 (16%)
		WD 8	424..464		18/41 (44%)
		WD40 repeat	429..468	CDD:293791	16/40 (40%)
		WD 9	468..509		8/40 (20%)
		WD40 repeat	474..538	CDD:293791	8/73 (11%)
		WD 10	533..573		9/58 (16%)
		WD40 repeat	542..575	CDD:293791	6/41 (15%)
		WD 11	576..622		12/52 (23%)
		WD40 repeat	582..636	CDD:293791	12/60 (20%)
		WD 12	637..677		12/42 (29%)
		WD40 repeat	642..679	CDD:293791	10/37 (27%)
		WD40	673..710	CDD:197651	7/40 (18%)
		WD 13	680..720		9/44 (20%)
		WD40 repeat	685..722	CDD:293791	8/41 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	715..790		19/93 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1313..1343
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1389..1428
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	80	1.000	Inparanoid score	I5231
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D283127at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_106770
Panther	1	1.100	-	-	LDO	PTHR46362
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	7	6.970