DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpA and CAPN3

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_000061.1	Gene:	CAPN3 / 825	HGNCID:	1480	Length:	821	Species:	Homo sapiens

Alignment Length:	873	Identity:	340/873 - (38%)
Similarity:	485/873 - (55%)	Gaps:	164/873 - (18%)

- Green bases have known domain annotations that are detailed below.

  Fly    60 PLQSQSNSTLYYGNLGEKSSSLGPYSEV------------QDYETILNSCLASGSLFEDPLFPAS 112
            |..:||.:|...|  |..|   |.||.:            :.:|.:...||....|:.||.||..
Human    24 PHPAQSKATEAGG--GNPS---GIYSAIISRNFPIIGVKEKTFEQLHKKCLEKKVLYVDPEFPPD 83

  Fly   113 NESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRV 177
            ..||.:|::......|.||.||.|||:|.::|.:|.|:.||||||||.|||.|.||...:|.|||
Human    84 ETSLFYSQKFPIQFVWKRPPEICENPRFIIDGANRTDICQGELGDCWFLAAIACLTLNQHLLFRV 148

  Fly   178 IPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLH 242
            ||.:|||.|||||||||:||:||:||||:|||.|||||.:|::..|..:||||||||||||||||
Human   149 IPHDQSFIENYAGIFHFQFWRYGEWVDVVIDDCLPTYNNQLVFTKSNHRNEFWSALLEKAYAKLH 213

  Fly   243 GSYEALKGGSTCEAMEDFTGGVSEWYDLKEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETP 307
            |||||||||:|.||||||||||:|::::::||.:::.|::||.||.|:|||||:...|:|...:|
Human   214 GSYEALKGGNTTEAMEDFTGGVAEFFEIRDAPSDMYKIMKKAIERGSLMGCSIDDGTNMTYGTSP 278

  Fly   308 Q-------------------------------------------------GLIRGHAYSITKVCL 323
            .                                                 ||:||||||:|.
Human   279 SGLNMGELIARMVRNMDNSLLQDSDLDPRGSDERPTRTIIPVQYETRMACGLVRGHAYSVTG--- 340

  Fly   324 IDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQD 388
            :|.| |.:..|:.::|:||||| :.||||.|||...:|.::.:::||.:......|||||||::|
Human   341 LDEV-PFKGEKVKLVRLRNPWG-QVEWNGSWSDRWKDWSFVDKDEKARLQHQVTEDGEFWMSYED 403

  Fly   389 FLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITL 453
            |:.||.::|||||:.|:|..|:..:    |.:|:.||.|..|.:|||||||.||||.||||.:.|
Human   404 FIYHFTKLEICNLTADALQSDKLQT----WTVSVNEGRWVRGCSAGGCRNFPDTFWTNPQYRLKL 464

  Fly   454 V--DPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGL--NFFRYK 514
            :  |.|.:|.|..|:.:|||||||||..|.:|....|||||||.: .:|:....|.|  :||.|.
Human   465 LEEDDDPDDSEVICSFLVALMQKNRRKDRKLGASLFTIGFAIYEV-PKEMHGNKQHLQKDFFLYN 528

  Fly   515 SSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGK 579
            :|..||..:||.|||..||:|||..|:|||||::|::|||||:|||||.:|..||.::.:.
Human   529 ASKARSKTYINMREVSQRFRLPPSEYVIVPSTYEPHQEGEFILRVFSEKRNLSEEVENTIS---- 589

  Fly   580 ADTITPGFPTPKPIDPQKEGLRRLFDSIAGKDMEVDWMELKRILDHSMRDDLPKPVVFNRFSNNM 644
                     ..:|:..:|                                  .||::|.....|.
Human   590 ---------VDRPVKKKK----------------------------------TKPIIFVSDRANS 611

  Fly   645 AFETQAAGPGDDGAGACGLLSLICGPFLKGTPFEE------QLGMNDQSN----------KRLIG 693
            ..|.......::|.|             |.:|.::      |.|.:||.:          |::.|
Human   612 NKELGVDQESEEGKG-------------KTSPDKQKQSPQPQPGSSDQESEEQQQFRNIFKQIAG 663

  Fly   694 DNP---ADGGPVTANAIVD-----ETHGFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWK 750
            |:.   ||......|.:|:     :||||:.:.||||:|::|.|.||||..:||..|.::|..|:
Human   664 DDMEICADELKKVLNTVVNKHKDLKTHGFTLESCRSMIALMDTDGSGKLNLQEFHHLWNKIKAWQ 728

  Fly   751 AIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYI 815
            .|||.||.:.:|.::.:::|.|:|.||:||||::.:::..||..:...|.||.||.|.|:::...
Human   729 KIFKHYDTDQSGTINSYEMRNAVNDAGFHLNNQLYDIITMRYADKHMNIDFDSFICCFVRLEGMF 793

  Fly   816 DIFKERDTEKNETATFTLEEWIERTIYS 843
            ..|...|.:.:......:.||::.|:|:
Human   794 RAFHAFDKDGDGIIKLNVLEWLQLTMYA 821

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	164/344 (48%)
		Calpain_III	418..565	CDD:279416	81/150 (54%)
		EFh	719..765	CDD:238008	22/45 (49%)
CAPN3	NP_000061.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	7..37		5/14 (36%)
		Peptidase_C2	75..415	CDD:279042	164/344 (48%)
		Domain III	418..586		87/172 (51%)
		Calpain_III	429..579	CDD:279416	81/150 (54%)
		Calpain_u2	583..653	CDD:293254	16/129 (12%)
		Linker	587..649		15/121 (12%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	609..652		10/55 (18%)
		Domain IV	650..821		56/170 (33%)
		EFh	653..721	CDD:238008	24/67 (36%)
		EFh	697..751	CDD:238008	23/53 (43%)
		EFh	728..817	CDD:298682	28/88 (32%)
		EF-hand_7	728..784	CDD:290234	21/55 (38%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	356	1.000	Domainoid score	I986
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	643	1.000	Inparanoid score	I809
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48339
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm8590
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	12	11.880