DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpA and CAPN1

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_001185797.1	Gene:	CAPN1 / 823	HGNCID:	1476	Length:	714	Species:	Homo sapiens

Alignment Length:	774	Identity:	318/774 - (41%)
Similarity:	449/774 - (58%)	Gaps:	117/774 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    88 QDYETILNSCLASGSLFEDPLFPASNESLQF-----SRRPDRHIEWLRPHEIAENPQFFVEGYSR 147
            ||||.:...||.||:||.|..||...:||.:     :......|:|.||.|:..||||.|:|.:|
Human    40 QDYEQLRVRCLQSGTLFRDEAFPPVPQSLGYKDLGPNSSKTYGIKWKRPTELLSNPQFIVDGATR 104

  Fly   148 FDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLP 212
            .|:.||.|||||||||.|:||....|..||:|..|||:..|||||||:.||:|:||||::||.||
Human   105 TDICQGALGDCWLLAAIASLTLNDTLLHRVVPHGQSFQNGYAGIFHFQLWQFGEWVDVVVDDLLP 169

  Fly   213 TYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDLKEAPGNL 277
            ..:|:|:::||.|.||||||||||||||::||||||.||||.|..|||||||:|||:|::||.:|
Human   170 IKDGKLVFVHSAEGNEFWSALLEKAYAKVNGSYEALSGGSTSEGFEDFTGGVTEWYELRKAPSDL 234

  Fly   278 FTILQKAAERNSMMGCSIEPDPNV-TEAETPQGLIRGHAYSITKVCLIDIVTPNRQGK-IPMIRM 340
            :.|:.||.||.|::||||:....: .||.|.:.|::|||||:|....:     |.:|: :.:|||
Human   235 YQIILKALERGSLLGCSIDISSVLDMEAITFKKLVKGHAYSVTGAKQV-----NYRGQVVSLIRM 294

  Fly   341 RNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDS 405
            ||||| |.||.|.|||||.||..:...::.::.:..: ||||||||:||:..|.|:|||||:||:
Human   295 RNPWG-EVEWTGAWSDSSSEWNNVDPYERDQLRVKME-DGEFWMSFRDFMREFTRLEICNLTPDA 357

  Fly   406 LTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQ----CT 466
            |    ::...|||..::|||.|..|.|||||||:..|||.|||:.|.|.:.|:.|:.|.    |:
Human   358 L----KSRTIRKWNTTLYEGTWRRGSTAGGCRNYPATFWVNPQFKIRLDETDDPDDYGDRESGCS 418

  Fly   467 VIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQ---GLNFFRYKSSVGRSPHFINTRE 528
            .::|||||:||.:|..|.:..|||||:|.: ..||..:|.   ..:||...:|..||..|||.||
Human   419 FVLALMQKHRRRERRFGRDMETIGFAVYEV-PPELVGQPAVHLKRDFFLANASRARSEQFINLRE 482

  Fly   529 VCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPI 593
            |..||:||||.|::|||||:||:||:|::|.|||......|.||.         |....|..:.:
Human   483 VSTRFRLPPGEYVVVPSTFEPNKEGDFVLRFFSEKSAGTVELDDQ---------IQANLPDEQVL 538

  Fly   594 DPQK--EGLRRLFDSIAGKDMEVDWMELKRILDH--SMRDDLPKPVVFNRFSNNMAFETQAAGPG 654
            ..::  |..:.||..:||:|||:...||:.||:.  |...||
Human   539 SEEEIDENFKALFRQLAGEDMEISVKELRTILNRIISKHKDL----------------------- 580

  Fly   655 DDGAGACGLLSLICGPFLKGTPFEEQLGMNDQSNKRLIGDNPADGGPVTANAIVDETHGFSKDVC 719
                                                                   .|.|||.:.|
Human   581 -------------------------------------------------------RTKGFSLESC 590

  Fly   720 RSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRV 784
            ||||.::|.|.:||||..||..|.:.|..:.:||:.:|::.:|.:|.:::|.|:.|||:.||.::
Human   591 RSMVNLMDRDGNGKLGLVEFNILWNRIRNYLSIFRKFDLDKSGSMSAYEMRMAIESAGFKLNKKL 655

  Fly   785 LNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIYS 843
            ..::..||...|..:.||:|:.|.|:::|....||..||:.:...||.|.:|::.|:::
Human   656 YELIITRYSEPDLAVDFDNFVCCLVRLETMFRFFKTLDTDLDGVVTFDLFKWLQLTMFA 714

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	157/302 (52%)
		Calpain_III	418..565	CDD:279416	75/153 (49%)
		EFh	719..765	CDD:238008	19/45 (42%)
CAPN1	NP_001185797.1	Peptidase_C2	56..352	CDD:306994	157/302 (52%)
		Domain III	355..526		81/175 (46%)
		Calpain_III	364..524	CDD:238132	77/160 (48%)
		Linker	527..542		2/23 (9%)
		Domain IV	543..713		64/247 (26%)
		EFh_PEF_CAPN1	546..714	CDD:320073	63/245 (26%)
		EF-hand motif	546..574	CDD:320073	11/27 (41%)
		EF-hand motif	589..618	CDD:320073	14/28 (50%)
		EF-hand motif	619..649	CDD:320073	9/29 (31%)
		EF-hand motif	655..683	CDD:320073	8/27 (30%)
		EF-hand motif	685..714	CDD:320073	9/28 (32%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	356	1.000	Domainoid score	I986
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	643	1.000	Inparanoid score	I809
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm8590
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	11	10.870