DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment CalpA and si:dkeyp-50d11.2

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	XP_009293787.1	Gene:	si:dkeyp-50d11.2 / 565825	ZFINID:	ZDB-GENE-091113-22	Length:	704	Species:	Danio rerio

Alignment Length:	770	Identity:	320/770 - (41%)
Similarity:	443/770 - (57%)	Gaps:	114/770 - (14%)

- Green bases have known domain annotations that are detailed below.

  Fly    88 QDYETILNSCLASGSLFEDPLFPASNESLQF-----SRRPDRHIEWLRPHEIAENPQFFVEGYSR 147
            ||:|::...||.||.||||.:|||...||.|     :....|.:.|:||.|...:|.|.|:|.:|
Zfish    35 QDFESLRTRCLQSGHLFEDDIFPAQQSSLGFKELGPNSSKTRGVRWMRPTEFCSDPHFIVDGATR 99

  Fly   148 FDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLP 212
            .|:.||.|||||||||.|.||....|..||:|..|||.:.|||||||:|||:|.||||:||||||
Zfish   100 TDICQGALGDCWLLAAIACLTLNEPLLRRVVPHGQSFHQQYAGIFHFQFWQFGDWVDVVIDDRLP 164

  Fly   213 TYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDLKEAPGNL 277
            ..:|:|:::||.|.:||||||:|||||||:|.||||.||||.|..|||||||:|.|:||.||.||
Zfish   165 VRDGKLLFVHSAEGSEFWSALVEKAYAKLNGCYEALSGGSTSEGFEDFTGGVTEMYELKNAPPNL 229

  Fly   278 FTILQKAAERNSMMGCSIEPDPNVT-----EAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPM 337
            |:|:.:|.||.|:|||||:    :|     ||.|.:.|::|||||||.|..::.    |:.:..:
Zfish   230 FSIITRAVERGSLMGCSID----ITSFFDMEAITFKKLVKGHAYSITGVDEVEY----RRSQTKL 286

  Fly   338 IRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLS 402
            :|:||||| |.||.|||||.|.|||.|....::.:. ....||||||:|.||...|.|:|||||:
Zfish   287 LRIRNPWG-EVEWTGPWSDESKEWREIDASVRSRLH-NCQEDGEFWMAFSDFKREFSRLEICNLT 349

  Fly   403 PDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTV 467
            .|:|    |:...:||..|:|.|||..|.|||||||:..|||.|||:.|.|.:||.:::|| ||.
Zfish   350 ADAL----QSREVKKWNSSLYPGEWRRGSTAGGCRNYPATFWINPQFKIVLKEPDTDNQEG-CTF 409

  Fly   468 IVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGL--NFFRYKSSVGRSPHFINTREVC 530
            :|||||||||..|..|.:..|||||||.:....|......|  :||...:|..||..|||.|||.
Zfish   410 LVALMQKNRRQLRREGKDMETIGFAIYEVPKEYLGRVGVHLKRDFFLKHTSSARSELFINLREVS 474

  Fly   531 ARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPIDP 595
            :||.||.|.|:||||||:|.:||:|::|||||...:.:|.||.:    .||  .|..|..:..|.
Zfish   475 SRFSLPAGEYIIVPSTFEPQKEGDFVLRVFSEKATDSQELDDEI----SAD--VPEEPVLQESDI 533

  Fly   596 QKEGLRRLFDSIAGKDMEVDWMELKRILDH--SMRDDLPKPVVFNRFSNNMAFETQAAGPGDDGA 658
            . ||.:.||..:||.:||::..:|:.||:.  |...|:
Zfish   534 D-EGFKALFAKLAGPEMEINVAKLEMILNRVVSKHKDI--------------------------- 570

  Fly   659 GACGLLSLICGPFLKGTPFEEQLGMNDQSNKRLIGDNPADGGPVTANAIVDETHGFSKDVCRSMV 723
                                                               :|.||.|:.||.|:
Zfish   571 ---------------------------------------------------KTDGFGKETCRGMI 584

  Fly   724 AMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVL 788
            .::|...:||||..:|..|..:..::.|:|:.:|::.:|.:|.:::|.||.|||:.|.|.:..::
Zfish   585 NLMDTTGTGKLGLTDFHVLWEKFKRYLAVFREFDIDKSGTMSSYEMRLALESAGFKLTNNLFQLI 649

  Fly   789 GHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIYS 843
            ..||...|..:.||:|:.|.::::|....||..|.:::...:|:..:||..|:::
Zfish   650 ILRYAKSDLNVDFDNFVACLIRLETMFKTFKTLDADEDGLVSFSFAQWITLTMFT 704

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	162/305 (53%)
		Calpain_III	418..565	CDD:279416	80/148 (54%)
		EFh	719..765	CDD:238008	14/45 (31%)
si:dkeyp-50d11.2	XP_009293787.1	Peptidase_C2	51..347	CDD:279042	162/305 (53%)
		Calpain_III	361..507	CDD:279416	79/146 (54%)
		EF1B	500..563	CDD:294128	24/69 (35%)
		EFh	580..630	CDD:298682	15/49 (31%)
		EFh	612..667	CDD:238008	19/54 (35%)
		EF-hand_7	613..698	CDD:290234	24/84 (29%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	358	1.000	Domainoid score	I921
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	656	1.000	Inparanoid score	I755
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm6388
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	10	9.970