DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpA and capn2b

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_001018177.1	Gene:	capn2b / 563053	ZFINID:	ZDB-GENE-050522-84	Length:	696	Species:	Danio rerio

Alignment Length:	792	Identity:	309/792 - (39%)
Similarity:	441/792 - (55%)	Gaps:	129/792 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    63 SQSNSTLYYGNLGEKSSSLGPYSEVQDYETILNSCLASGSLFEDPLFPASNESLQF-----SRRP 122
            :.:|:|.|..               ||:|.:.:.|.|.|:||.||.|||:.|||.|     :...
Zfish    20 TNANATKYLN---------------QDFEALRSECRARGTLFSDPTFPAAPESLGFKELGPNSSK 69

  Fly   123 DRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEEN 187
            .|.:.|.||.|:.:.|:|.|.|.::.|:.||.|||||||||.|:|||..::..||:|..|.|:..
Zfish    70 TRGLVWKRPGELCQKPRFIVGGATKTDICQGALGDCWLLAAIASLTQNEDVLARVVPNGQEFDGT 134

  Fly   188 YAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGS 252
            |||||||:|||:|:||||:||||||..:|||::::|.||||||||||||||||::|.||||.|||
Zfish   135 YAGIFHFQFWQFGEWVDVVIDDRLPVKDGELLFVYSAEKNEFWSALLEKAYAKVNGCYEALSGGS 199

  Fly   253 TCEAMEDFTGGVSEWYDLKEAPGNLFTILQKAAERNSMMGCSIEPDPNVT-----EAETPQGLIR 312
            |.|..||||||::|.|::::||.|||.|:|||.|..:::||||:    :|     ||.|.|.|::
Zfish   200 TSEGFEDFTGGIAESYEIRKAPTNLFQIIQKALEAGALLGCSID----ITSAADSEAITRQKLVK 260

  Fly   313 GHAYSITKVCLIDIVTPNRQGKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFD 377
            |||||:|....::.    |..|..::||||||| :.||.|.|||:||||..:...::.:...
Zfish   261 GHAYSLTGATEVNY----RGRKEKLVRMRNPWG-QVEWTGAWSDNSPEWNSVAASERPDASA--- 317

  Fly   378 RDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDT 442
            .||||||:|.:||.::.|:|||.|:||::|:|    ..:.|.::.::|.|..|.|||||||...|
Zfish   318 EDGEFWMAFSEFLTNYSRIEICTLTPDAITDD----SVKHWAVTNHDGTWRRGSTAGGCRNNPYT 378

  Fly   443 FWHNPQYIITLVDPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQG 507
            ||.|||:::.|.:.:.||:|..|:.:|.|:|||||..|..|.:..|||||||.|.......|...
Zfish   379 FWMNPQFVVKLDEENVEDKEKGCSFVVGLIQKNRRRLRKTGEDMNTIGFAIYELPQEFHGQREVH 443

  Fly   508 L--NFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEEN 570
            |  |||.......||..|||.||||.||.||||.||||||||:.|::|:|.:|||||:|:..:..
Zfish   444 LDKNFFLTHGQKARSETFINLREVCTRFHLPPGEYLIVPSTFEANKDGDFCLRVFSESQSETQHC 508

  Fly   571 DDHVGYGGKADTITPGFPTPKPIDPQKEG-LRRLFDSIAGKDMEVDWMELKRILDHSMRDDLPKP 634
            ||.|......:|::         |.|.:. .|.:|..:||.|||:..:||.
Zfish   509 DDPVDAELPDETVS---------DDQVDSKFRGMFVKLAGPDMEISPLELM-------------- 550

  Fly   635 VVFNRFSNNMAFETQAAGPGDDGAGACGLLSLICGPFLKGTPFEEQLGMNDQSNKRLIGDNPADG 699
            .:||:
Zfish   551 TIFNK------------------------------------------------------------ 555

  Fly   700 GPVTANAIVDETHGFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRV 764
              :.|.....::..|:.|.||.||.::|...:||||..||.||..::.::..|||..|::::|.:
Zfish   556 --IIAKRTDIKSSTFTLDTCRVMVNLMDDSGNGKLGLGEFATLWKKVQRYLEIFKHNDLDSSGTI 618

  Fly   765 SGFQLREALNSAGYHLNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETA 829
            |..:||.||..||:.|||.:..::..||..:|..:.||:|:.|.::::.....||..|..|:...
Zfish   619 STPELRMALKEAGFCLNNTLFQLMVARYAEKDMTLLFDNFVSCLMRLEMMFRAFKRLDPHKSGFI 683

  Fly   830 TFTLEEWIERTI 841
            .....:|:..|:
Zfish   684 ELNFNQWLNLTM 695

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	156/305 (51%)
		Calpain_III	418..565	CDD:279416	75/148 (51%)
		EFh	719..765	CDD:238008	18/45 (40%)
capn2b	NP_001018177.1	Peptidase_C2	46..339	CDD:279042	155/304 (51%)
		Calpain_III	354..503	CDD:279416	75/148 (51%)
		EFh	573..628	CDD:238008	22/54 (41%)
		FRQ1	601..>686	CDD:227455	26/84 (31%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	358	1.000	Domainoid score	I921
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	656	1.000	Inparanoid score	I755
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm6388
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.870