DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment CalpA and capn2a

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_001013519.1	Gene:	capn2a / 541374	ZFINID:	ZDB-GENE-050320-69	Length:	698	Species:	Danio rerio

Alignment Length:	771	Identity:	306/771 - (39%)
Similarity:	430/771 - (55%)	Gaps:	128/771 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    88 QDYETILNSCLASGSLFEDPLFPASNESLQFSR-----RPDRHIEWLRPHEIAENPQFFVEGYSR 147
            |::||:.:.||:.|.||.||.|||:.|:|.|:.     ...|.:||.||.::..:|:|.|.|.:|
Zfish    30 QNFETLRSECLSRGELFCDPAFPAAPEALGFNELGPRSSKTRGVEWKRPGQLNSSPEFIVGGATR 94

  Fly   148 FDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLP 212
            .|:.||.|||||||||.|:||...::..||:|::|.|.::|||||||:|||:|:||||::|||||
Zfish    95 TDICQGALGDCWLLAAIASLTLNEDVLARVVPSDQGFGQDYAGIFHFQFWQFGEWVDVVVDDRLP 159

  Fly   213 TYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDLKEAPGNL 277
            |.:|||:::||...:|||||||||||||::|.||||.||||.|..||||||::|.|:||.||.||
Zfish   160 TRDGELLFVHSVTGSEFWSALLEKAYAKVNGCYEALSGGSTTEGFEDFTGGIAEMYELKSAPTNL 224

  Fly   278 FTILQKAAERNSMMGCSIEPDPNVT-----EAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPM 337
            |.|::||.:..:::||||:    :|     ||.|.|.|::|||||:|....::.    |..|..:
Zfish   225 FQIIKKALDSGALLGCSID----ITSAADSEAVTYQKLVKGHAYSLTGATEVNY----RSRKEKL 281

  Fly   338 IRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLS 402
            :|:||||| :.||.|.|||:|.||..:...::..:.   ..||||||||.||:..:.|:|||.|:
Zfish   282 VRVRNPWG-QVEWTGAWSDNSSEWNAVDPSERENVK---SEDGEFWMSFSDFVRQYSRLEICTLT 342

  Fly   403 PDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITL--VDPDEEDEEGQC 465
            ||:||.|    ..:.|.:..::|.|..|.|||||||...|||.|||:.|.|  .|.|.:|.|..|
Zfish   343 PDTLTSD----SLKHWSVCKFDGTWRKGSTAGGCRNHPYTFWMNPQFKIKLEEEDDDPDDNEVGC 403

  Fly   466 TVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGL--NFFRYKSSVGRSPHFINTRE 528
            :|::.|:|||||..|..|.:..|||:|||.:..:....:...|  |:|...:...||..|||.||
Zfish   404 SVVIGLIQKNRRKMRKAGEDMHTIGYAIYEVPSQFHGQKEVHLDKNYFLTHAQKARSETFINLRE 468

  Fly   529 VCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPI 593
            |..|||||||.||||||||||::.|:|.:|||||.|:.|::.||               |....:
Zfish   469 VSTRFKLPPGEYLIVPSTFDPHKNGDFCVRVFSEKQSEMQQCDD---------------PIEANV 518

  Fly   594 DPQ-------KEGLRRLFDSIAGKDMEVDWMELKRILDHSMRDDLPKPVVFNRFSNNMAFETQAA 651
            |.:       ..|.|.||..:||.|||:...||:              .:||:
Zfish   519 DDEMVSEEEVDSGFRGLFTKLAGDDMEISASELR--------------TIFNK------------ 557

  Fly   652 GPGDDGAGACGLLSLICGPFLKGTPFEEQLGMNDQSNKRLIGDNPADGGPVTANAIVDETHGFSK 716
                                                              :.|.....:|.|||.
Zfish   558 --------------------------------------------------IVAKRTDIKTDGFSL 572

  Fly   717 DVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLN 781
            |.||.||.::|...:||||..||.||..:|.|:..|:|..|::.:|.:|..::|.||..||:.||
Zfish   573 DTCRVMVNLMDESGNGKLGLTEFATLWKKIQKYLGIYKKNDMDGSGCMSTPEMRMALKEAGFSLN 637

  Fly   782 NRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWI 837
            :.:...|..|||..|..|.||:|:.|.::::....:||..|.:.:........:|:
Zfish   638 DCIHQSLAARYGDADMTIDFDNFVSCVMRLEMMFKVFKRMDIDHSGFIELDFFQWL 693

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	152/305 (50%)
		Calpain_III	418..565	CDD:279416	74/150 (49%)
		EFh	719..765	CDD:238008	19/45 (42%)
capn2a	NP_001013519.1	Peptidase_C2	46..339	CDD:279042	151/304 (50%)
		Calpain_III	354..505	CDD:279416	74/150 (49%)
		EF-hand_8	542..601	CDD:290545	26/134 (19%)
		EFh	575..630	CDD:298682	22/54 (41%)
		FRQ1	602..>694	CDD:227455	28/92 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	358	1.000	Domainoid score	I921
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	656	1.000	Inparanoid score	I755
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm6388
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	10	9.970