DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpA and CAPN14

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_001138594.1	Gene:	CAPN14 / 440854	HGNCID:	16664	Length:	684	Species:	Homo sapiens

Alignment Length:	779	Identity:	260/779 - (33%)
Similarity:	382/779 - (49%)	Gaps:	125/779 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    78 SSSLGPYSEVQDYETILNSCLASGSLFEDPLFPASNESL----QFSRRPDRHIEWLRPHEIAENP 138
            |....|....||:|.:|..||.:|.||||..|||:..|:    ...:.|.| ::|.||.|:..||
Human    18 SRRASPQQPQQDFEALLAECLRNGCLFEDTSFPATLSSIGSGSLLQKLPPR-LQWKRPPELHSNP 81

  Fly   139 QFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWV 203
            ||:.....|.|:.||.:||||.|||...|....::..||:|..|||.|.|||||.|.||.||.||
Human    82 QFYFAKAKRLDLCQGIVGDCWFLAALQALALHQDILSRVVPLNQSFTEKYAGIFRFWFWHYGNWV 146

  Fly   204 DVIIDDRLPTYN-GELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEW 267
            .|:||||||... |:|:::.||.||.||.||||||||||.||||.|:.|...||:.||||||:..
Human   147 PVVIDDRLPVNEAGQLVFVSSTYKNLFWGALLEKAYAKLSGSYEDLQSGQVSEALVDFTGGVTMT 211

  Fly   268 YDLKEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAETPQGLIRGHAYSITKVCLIDIVTPNRQ 332
            .:|.||.|||:.||.:|....:::||.......:.|    .||:.||||::|.   |..||...:
Human   212 INLAEAHGNLWDILIEATYNRTLIGCQTHSGEKILE----NGLVEGHAYTLTG---IRKVTCKHR 269

  Fly   333 GKIPMIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVE 397
            .:. ::::||||| :.||.|.|||||.:|..:..::|..: |..|.||||||:.|||..||..:.
Human   270 PEY-LVKLRNPWG-KVEWKGDWSDSSSKWELLSPKEKILL-LRKDNDGEFWMTLQDFKTHFVLLV 331

  Fly   398 ICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFL-DTFWHNPQYIITLVDPDEEDE 461
            ||.|:|..|:::    ..:||..:|.||.|....||||.|..| ||||.|||:::::..|    |
Human   332 ICKLTPGLLSQE----AAQKWTYTMREGRWEKRSTAGGQRQLLQDTFWKNPQFLLSVWRP----E 388

  Fly   462 EGQ-----CTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGLNFFRYKSSVGRSP 521
            ||:     |:|:|:|:||.|...|.. ...|.|||.:|.:|....:.|.....||:..:.:.:..
Human   389 EGRRSLRPCSVLVSLLQKPRHRCRKR-KPLLAIGFYLYRMNKYHDDQRRLPPEFFQRNTPLSQPD 452

  Fly   522 HFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGY--GGKADTIT 584
            .|:..:||.....|.||.|||||...:.:::.||::||||.         .|:.|  |..:
Human   453 RFLKEKEVSQELCLEPGTYLIVPCILEAHQKSEFVLRVFSR---------KHIFYEIGSNS---- 504

  Fly   585 PGFPTPKPIDPQKEGLRRLFDSIAGKDMEVDWMELKRILDHSMRDDLPKPVVFNRFSNNMAFETQ 649
             |....|.|:.|.|.....|.....|..|::.::|:.:|                  |.|.:.:.
Human   505 -GVVFSKEIEDQNERQDEFFTKFFEKHPEINAVQLQNLL------------------NQMTWSSL 550

  Fly   650 AAGPGDDGAGACGLLSLICGPFLKGTPFEEQLGMNDQSNKRLIGDNPADGGPVTANAIVDETHGF 714
            .:                      ..||                                    |
Human   551 GS----------------------RQPF------------------------------------F 557

  Fly   715 SKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYH 779
            |.:.|:.::|:||.:.||.:..:||..|..::...:.:|...| ..:|.::..||..|:..||..
Human   558 SLEACQGILALLDLNASGTMSIQEFRDLWKQLKLSQKVFHKQD-RGSGYLNWEQLHAAMREAGIM 621

  Fly   780 LNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIYS 843
            |::.|..::..|||....::.|..||...::::...|:|:.. |:..:.......||:...:||
Human   622 LSDDVCQLMLIRYGGPRLQMDFVSFIHLMLRVENMEDVFQNL-TQDGKGIYLQKPEWMMMALYS 684

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	138/300 (46%)
		Calpain_III	418..565	CDD:279416	56/152 (37%)
		EFh	719..765	CDD:238008	12/45 (27%)
CAPN14	NP_001138594.1	Peptidase_C2	44..334	CDD:279042	138/300 (46%)
		Domain III	337..503		62/183 (34%)
		Calpain_III	348..496	CDD:279416	56/161 (35%)
		Linker	504..517		4/17 (24%)
		Domain IV	518..683		41/242 (17%)
		EFh	562..616	CDD:298682	15/54 (28%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	8	7.880