DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpA and capn1

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_998624.1	Gene:	capn1 / 406768	ZFINID:	ZDB-GENE-040426-2814	Length:	704	Species:	Danio rerio

Alignment Length:	774	Identity:	326/774 - (42%)
Similarity:	445/774 - (57%)	Gaps:	122/774 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    88 QDYETILNSCLASGSLFEDPLFPASNESLQFSRRPDRH-----IEWLRPHEIAENPQFFVEGYSR 147
            |||||:......|..|||||:|.||:.||.|:....|.     :.|:||.|:...|||.|:|.:|
Zfish    35 QDYETLRAQSQQSRRLFEDPMFTASSSSLGFNELGPRSSKTQGVRWMRPKEMCARPQFIVDGATR 99

  Fly   148 FDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQSFEENYAGIFHFRFWQYGKWVDVIIDDRLP 212
            .|:.||.|||||||||.|:||...||..||:|..|.|:..|||||||:|||:|:||||:||||||
Zfish   100 TDICQGALGDCWLLAAIASLTLNDNLLHRVVPHGQDFDSRYAGIFHFQFWQFGEWVDVVIDDRLP 164

  Fly   213 TYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDLKEAPGNL 277
            |.:|:|:::||.|..||||||||||||||:|.||||.||||||..|||||||:|.|:||:||.:|
Zfish   165 TKDGKLLFVHSAEGGEFWSALLEKAYAKLNGCYEALSGGSTCEGFEDFTGGVTEMYELKKAPADL 229

  Fly   278 FTILQKAAERNSMMGCSIEPDPNVT-----EAETPQGLIRGHAYSITKVCLIDIVTPNRQGKIPM 337
            |:|:.:|.||.|::||||:    :|     ||.|.:.|::|||||:|..  .::|......|  :
Zfish   230 FSIIGRAIERGSLLGCSID----ITSKFDMEAVTFKKLVKGHAYSVTGA--EEVVYRGNMTK--L 286

  Fly   338 IRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGLTFDRDGEFWMSFQDFLNHFDRVEICNLS 402
            :|:||||| |.||.|.|||:|.||..:....:..: .....||||||||.|||..|.|:|||||:
Zfish   287 VRIRNPWG-EVEWTGAWSDNSREWDNVDRSVRGRL-QNRSEDGEFWMSFSDFLREFTRLEICNLT 349

  Fly   403 PDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTV 467
            .|:|    |.|..:||..:.|.|||..|.|||||||:..|||:|||:.:.|..||... :.:|:.
Zfish   350 ADAL----QASQVKKWSTANYNGEWRRGSTAGGCRNYPATFWNNPQFKVALKHPDSPG-QSECSF 409

  Fly   468 IVALMQKNRRSKRNMGMECLTIGFAIYSLNDRELENRPQGL----NFFRYKSSVGRSPHFINTRE 528
            :||||||:||.||..|.:..|||||||.: .||...: .|:    :||...:|..||..|||.||
Zfish   410 LVALMQKDRRKKRREGQDMETIGFAIYEV-PREFLGQ-SGVHLKRDFFLTHASSARSELFINLRE 472

  Fly   529 VCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPI 593
            |.:||:||.|.|:||||||:||:|.:|::|||||...|.||.||.|         ....|..:.:
Zfish   473 VSSRFRLPAGEYIIVPSTFEPNKEADFVLRVFSEKPANSEEMDDKV---------MAEIPEEQRL 528

  Fly   594 DPQK--EGLRRLFDSIAGKDMEVDWMELKRILDH--SMRDDLPKPVVFNRFSNNMAFETQAAGPG 654
            |..:  .|.:.||..:||.|||:...||:.||:.  :...||
Zfish   529 DESQIDAGFKSLFRQLAGADMEISVTELQTILNRIIAKHKDL----------------------- 570

  Fly   655 DDGAGACGLLSLICGPFLKGTPFEEQLGMNDQSNKRLIGDNPADGGPVTANAIVDETHGFSKDVC 719
                                                                   :|.||.|:.|
Zfish   571 -------------------------------------------------------KTDGFGKESC 580

  Fly   720 RSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVYDVENTGRVSGFQLREALNSAGYHLNNRV 784
            |||:.::|.|.|||||..||..|..:|.::..||:.:||:.:|.:|.:::|:||.:||:.|||.:
Zfish   581 RSMINLVDTDGSGKLGLVEFHVLWEKIKRYLQIFRDHDVDKSGTMSSYEMRKALETAGFKLNNHL 645

  Fly   785 LNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKERDTEKNETATFTLEEWIERTIYS 843
            ..::..||...|..:.||:|:.|.|:::|....||..||:.:...:.|..:||..|:::
Zfish   646 FQLIILRYTEEDLSVDFDNFVSCLVRLETMFKTFKSLDTDADGVISLTFFQWITLTMFA 704

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	163/305 (53%)
		Calpain_III	418..565	CDD:279416	76/150 (51%)
		EFh	719..765	CDD:238008	20/45 (44%)
capn1	NP_998624.1	Peptidase_C2	51..347	CDD:279042	163/305 (53%)
		Calpain_III	361..509	CDD:279416	76/150 (51%)
		EF-hand_8	547..606	CDD:290545	27/136 (20%)
		EFh	580..634	CDD:238008	22/53 (42%)
		EF-hand_7	612..698	CDD:290234	28/85 (33%)
		EFh	613..692	CDD:298682	27/78 (35%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	358	1.000	Domainoid score	I921
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	656	1.000	Inparanoid score	I755
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm6388
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.870