DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpA and capn1a

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_956739.1	Gene:	capn1a / 393417	ZFINID:	ZDB-GENE-040426-1263	Length:	704	Species:	Danio rerio

Alignment Length:	800	Identity:	335/800 - (41%)
Similarity:	459/800 - (57%)	Gaps:	130/800 - (16%)

- Green bases have known domain annotations that are detailed below.

  Fly    71 YGNLGE-------KSSSLGPYSEV-----QDYETILNSCLASGSLFEDPLFPASNESLQFSR--- 120
            ||.:..       |:...|.:.:.     ||||.:...||..|.|||||.|||...||.|..
Zfish     4 YGGISAHISANRLKADGAGSFEQALHFQNQDYEALRQECLEGGYLFEDPCFPAEPPSLGFKELAP 68

  Fly   121 --RPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQS 183
              ...|.:||:||.|:.::|||.|.|.:|.|:.||.|||||||||..:||....|..||:|..||
Zfish    69 HSSKTRDVEWMRPTELCDDPQFIVGGATRTDICQGALGDCWLLAAIGSLTLNERLLHRVVPHGQS 133

  Fly   184 FEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEAL 248
            |:::|||||||:|||:|:|||::||||||..:||||::||.|.|||||||:|||||||:||||||
Zfish   134 FQDDYAGIFHFQFWQFGEWVDIVIDDRLPVKDGELMFVHSAEGNEFWSALVEKAYAKLNGSYEAL 198

  Fly   249 KGGSTCEAMEDFTGGVSEWYDLKEAPGNLFTILQKAAERNSMMGCSIEPDPNVT-----EAETPQ 308
            .||||.|..|||||||||.|:|::||.:|:.|:.||.:|.|::||||:    :|     |:.|.:
Zfish   199 SGGSTTEGFEDFTGGVSEMYELRKAPRDLYRIISKALDRGSLLGCSID----ITSAFDMESVTFK 259

  Fly   309 GLIRGHAYSITKVCLIDIVTPNRQGKIP-MIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEI 372
            .|::|||||:|.:..::.     :|::. :||:||||| :.||.|.|||:||||..|...:|.::
Zfish   260 KLVKGHAYSVTALKQVEY-----RGRMERLIRIRNPWG-QVEWTGAWSDNSPEWDEIDPSEKDDL 318

  Fly   373 GLTFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCR 437
            .|..: ||||||||.:||..|.|:|||||:||:|::|..:    .|....:.|.|..|.||||||
Zfish   319 HLQME-DGEFWMSFGEFLRQFSRLEICNLTPDALSDDDMS----HWNTIKFHGAWRRGSTAGGCR 378

  Fly   438 NFLDTFWHNPQYIITLV--DPDEEDEEGQCTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDR- 499
            |..:|||.||||.|||:  |.|.||||..|:.:||||||:||..|..|.:..|||||||.:.:.
Zfish   379 NHPNTFWINPQYKITLLEEDDDPEDEEVACSFLVALMQKDRRRYRGQGQDMHTIGFAIYEVPEEY 443

  Fly   500 -ELENRPQGLNFFRYKSSVGRSPHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFIIRVFSET 563
             ..:|.....:||...||..||..|||.|||..|.:||||.|:||||||:|::|.:|::|||:|.
Zfish   444 TGCQNVHLKKDFFLSNSSAARSETFINLREVSTRLRLPPGEYIIVPSTFEPSKEADFVLRVFTEK 508

  Fly   564 QNNMEENDDHVGYGGKADTITPGFPTPKPIDPQKEGLRRLFDSIAGKDMEVDWMELKRILDH--S 626
            |:..||.||.:    .||.......|...||   :..:.||..:||:|||:...|||.||:.  |
Zfish   509 QSETEELDDEI----SADLEDEEEITEDDID---DSFKSLFAQLAGEDMEISVHELKTILNRVVS 566

  Fly   627 MRDDLPKPVVFNRFSNNMAFETQAAGPGDDGAGACGLLSLICGPFLKGTPFEEQLGMNDQSNKRL 691
            ...|:
Zfish   567 KHKDV------------------------------------------------------------ 571

  Fly   692 IGDNPADGGPVTANAIVDETHGFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAIFKVY 756
                              :|.|||.|.||:||.:||.|.|.:||..||:.|.::|.|...||:.:
Zfish   572 ------------------KTDGFSMDSCRTMVNLLDKDGSARLGLVEFQILWNKIRKLLGIFREF 618

  Fly   757 DVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDIFKER 821
            |::.:|.:|.:::|.|:.|||:.||||:..:|..||...:. |.||:|:.|.:|::.....|::.
Zfish   619 DIDKSGTMSSYEMRLAVESAGFKLNNRLNQILVARYAENEA-IDFDNFVCCLIKLEAMFRSFQQL 682

  Fly   822 DTEKNETATFTLEEWIERTI 841
            |.|:..||...|.||:..|:
Zfish   683 DREETGTAEMNLSEWLFMTM 702

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	163/306 (53%)
		Calpain_III	418..565	CDD:279416	77/150 (51%)
		EFh	719..765	CDD:238008	19/45 (42%)
capn1a	NP_956739.1	Peptidase_C2	49..345	CDD:279042	163/306 (53%)
		Calpain_III	359..510	CDD:279416	77/150 (51%)
		EFh	581..631	CDD:298682	20/49 (41%)
		EFh	610..>664	CDD:298682	20/54 (37%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	358	1.000	Domainoid score	I921
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	656	1.000	Inparanoid score	I755
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm6388
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	11	10.870