Protein Alignment CalpA and clp-2

DIOPT Version :10

• Sequence 1: NP_001286613.1
• Gene: CalpA / 37232
• FlyBaseID: FBgn0012051
• Length: 843
• Species: Drosophila melanogaster
• Sequence 2: NP_497964.1
• Gene: clp-2 / 175618
• WormBaseID: WBGene00000543
• Length: 805
• Species: Caenorhabditis elegans
• Alignment Length: 740
• Identity: 152/740 - (20%)
• Similarity: 252/740 - (34%)
• Gaps: 261/740 - (35%)

  Fly    16 LNAAGEAAMGAAKDVVGSVINEIFIKKEADTKRVLPSIK-------NMRVQPL------------ 61
            |.:..|.|:...:|:|.      ..||..:.:.:.|.:.       |...||.
 Worm   136 LRSTAENALKCIEDLVA------LKKKVTEVELLFPEVPVDDLSNLNFNNQPSPSTPSNQPFKSP 194

  Fly    62 -----QSQSNSTLY----------YGNLGEKSSSLGPYSEVQDYETILNSCLASGSLFEDPLFPA 111
                 .|.|||..:          ..|:..|.     |....:|:.: |........|.||
 Worm   195 TRNFPPSPSNSQKFTKEELAVLFTTSNINNKL-----YVPFHNYDKV-NEFKGQQGKFTDP---- 249

  Fly   112 SNESLQFSRRPDRHIE-WLRPHEIAENPQ--FFVEGYSRFDVQQGELGDCWLLAATANLTQESNL 173
             ...:..:::....:: |.|..|:.|||.  |.::.::   ::|..:.||..:::.:........
 Worm   250 -EGKISLTQKQRMKLKCWKRVSELFENPTIIFSIDCHT---IKQTVISDCSFISSLSIAALYEKR 310

  Fly   174 FFRVIPAEQSFEE--------NYAGIFHFRFWQYGKWVDVIIDDRLPT-YNGELMYMHSTEKNEF 229
            |.:.:.....|.:        |.||.:.|:|...|.|..|:|||..|. .|..:|...:..|.|.
 Worm   311 FKKQLVTSIIFPQDANGKPIYNPAGKYMFKFHLNGAWRKVLIDDYFPVDENNRMMCSQTENKGEL 375

  Fly   230 WSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDL----KEAPGNLFTILQKAAERNSM 290
            |.:||||||.|:.|.|: ..|.::...:...||.:.|..:|    |..|..:|   :|..:|...
 Worm   376 WVSLLEKAYMKVMGGYD-FPGSNSNIDLNALTGWIPERIELSDTSKADPDEVF---RKLFDRFHR 436

  Fly   291 MGCSIE-PDPNVTE-AETPQGLIRGHAYSITKVCLIDI--VTPNRQGKIPMIRMRNPWGNEAEWN 351
            ..|.|. ....:|| .:...||:..|||::     |||  |...|     :::::||| ..:.|.
 Worm   437 GDCLITLATGKMTEDMQKRSGLVETHAYAV-----IDIRCVETKR-----LLKVKNPW-THSRWK 490

  Fly   352 GPWSDSSP-EWRYIPEEQKAEIGLTFD-------RDGEFWMSFQDFLNHFDRVEICNLSPDSLTE 408
            |.:||... .|     ..|.:..|.||       .||.||:.::...:.||.:.:
 Worm   491 GNFSDKDKVNW-----TAKMKNALAFDPEVAAEKDDGIFWIDYESVRHFFDVIYV---------- 540

  Fly   409 DQQNSGKRKWE------MSMYEGEWTPGVTAGGCRNFLDTFWHNPQYI----------------- 450
                    .|.      .|:|...||.  ..|..|: :.|...||||.
 Worm   541 --------NWNADLFPFKSVYHATWTQ--DTGPIRD-VYTVGENPQYTLTVNLNQKAAAVWILLT 594

  Fly   451 --ITLVDPDEEDEE---------GQ-----------------------C------------TVIV 469
              ||.:|....::|         ||                       |            |::|
 Worm   595 RHITAIDDFAVNKEFITLIVYETGQKIYIPSNPQPISDGVRINSPLYLCQLSNKKPGITKYTLVV 659

  Fly   470 ALMQKNRRSKRNMGMECLTIGFA--IYSLNDRELENRPQGLNFFRYKSSVG-------------- 518
            |..:|..           ||.::  ::|..|.:||  |..|.:...|::.|
 Worm   660 AQYEKTN-----------TINYSLRVFSTTDVKLE--PVQLPYSISKTTRGNWDGSDKYPIMKLT 711

  Fly   519 --------------RSPH-----------------FINTRE---------VCARFKLPPGHYLIV 543
                          ::|.                 |:.|:.         |....|:|.|.|.:.
 Worm   712 LHSKSDEIALFMELKAPKQFCVALEMKQNSSDRTVFLETKSSGAYRPGYTVLTLEKVPAGKYYVK 776

  Fly   544 PSTFDPNEEGEFIIRVFSETQNNME 568
            .||:...::|.||:|:.|..:.::|
 Worm   777 ISTYTAGDKGPFILRIDSTCKFDLE 801

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:459889	83/323 (26%)
		Calpain_III	416..569	CDD:238132	49/278 (18%)
		EFh_PEF_CalpA_B	600..843	CDD:320071
		EF-hand motif	600..627	CDD:320071
		EF-hand motif	717..747	CDD:320071
		EF-hand motif	748..778	CDD:320071
		EF-hand motif	784..812	CDD:320071
		EF-hand motif	813..843	CDD:320071
clp-2	NP_497964.1	MIT	4..75	CDD:239121
		CysPc	227..551	CDD:128526	87/370 (24%)
		Calpain_III	<722..801	CDD:469636	15/78 (19%)