DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CalpA and Capn8

DIOPT Version :9

Sequence 1:	NP_001286613.1	Gene:	CalpA / 37232	FlyBaseID:	FBgn0012051	Length:	843	Species:	Drosophila melanogaster
Sequence 2:	NP_570960.2	Gene:	Capn8 / 170725	MGIID:	2181366	Length:	703	Species:	Mus musculus

Alignment Length:	800	Identity:	337/800 - (42%)
Similarity:	459/800 - (57%)	Gaps:	138/800 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    59 QPLQSQSNSTLYYGNLGEKSSSLGPYSEVQDYETILNSCLASGSLFEDPLFPASNESLQF----- 118
            |.|.|..|:..|.|               ||:||:...||.||.||:||.|||...:|.:
Mouse    16 QGLGSNQNAVKYLG---------------QDFETLRKQCLNSGVLFKDPEFPACPSALGYRDLGP 65

  Fly   119 SRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESNLFFRVIPAEQS 183
            .....:.|.|.||.|:..||||.|.|.:|.|::||.|||||||||.|:||....|.:||:|.:||
Mouse    66 GSAETQGIIWKRPTELCSNPQFIVGGATRTDIRQGGLGDCWLLAAIASLTLNEKLLYRVVPRDQS 130

  Fly   184 FEENYAGIFHFRFWQYGKWVDVIIDDRLPTYNGELMYMHSTEKNEFWSALLEKAYAKLHGSYEAL 248
            |::||||||||:|||||:||:|:|||||||.||:|:::||.|.|||||||||||||||:||||||
Mouse   131 FQKNYAGIFHFQFWQYGEWVEVVIDDRLPTKNGQLLFLHSEEGNEFWSALLEKAYAKLNGSYEAL 195

  Fly   249 KGGSTCEAMEDFTGGVSEWYDLKEAPGNLFTILQKAAERNSMMGCSIEPDPNVTEAE--TPQGLI 311
            .||||.|..||||||:||:|||::.||||:..:|||..:.|::||||:.. |..|||  |.|.|:
Mouse   196 AGGSTIEGFEDFTGGISEFYDLRKPPGNLYYTIQKALRKGSLLGCSIDVS-NAAEAEATTRQKLV 259

  Fly   312 RGHAYSITKVCLIDIVTPNRQGKIP--MIRMRNPWGNEAEWNGPWSDSSPEWRYIPEEQKAEIGL 374
            :|||||:|.|..:|.     :| :|  :||:||||| |.||.|.||||:|||.||..::|.|:..
Mouse   260 KGHAYSVTGVEEVDF-----RG-LPEKLIRLRNPWG-EVEWTGAWSDSAPEWNYIDPQKKGELDK 317

  Fly   375 TFDRDGEFWMSFQDFLNHFDRVEICNLSPDSLTEDQQNSGKRKWEMSMYEGEWTPGVTAGGCRNF 439
            ..: ||||||||.|||..|.|:||||||||||:.::.:    ||.:.::.|.||.|.|||||:|:
Mouse   318 RAE-DGEFWMSFSDFLKQFSRLEICNLSPDSLSSEEIH----KWNLVLFNGRWTRGSTAGGCQNY 377

  Fly   440 LDTFWHNPQYIITLVDPDEEDEEGQ----CTVIVALMQKNRRSKRNMGMECLTIGFAIYSLNDRE 500
            ..|:|.|||:.|.|.:.||:.|||.    |||::.|||||||.:|.:|...|:||:|:|.: .:|
Mouse   378 PATYWTNPQFKIHLDEVDEDQEEGTSEPCCTVLLGLMQKNRRRQRRIGQGMLSIGYAVYQI-PKE 441

  Fly   501 LENRPQ---GLNFFRYKSSVGRSPH-----FINTREVCARFKLPPGHYLIVPSTFDPNEEGEFII 557
            |||...   |.:||:     ||.|.     ::|.|||.:|.:||||.||:|||||:|.::|:|.:
Mouse   442 LENHTDEHLGRDFFQ-----GRQPSTCSSTYMNLREVSSRVQLPPGQYLVVPSTFEPFKDGDFCL 501

  Fly   558 RVFSETQNNMEENDDHVGYGGKADTITPGFPTPKPIDPQKEGLRRLFDSIAGKDMEVDWMELKRI 622
            |||||.:....|..|.|.    .|   |..|.|:.:|.:.|....|.:..|.||.|:...:|||:
Mouse   502 RVFSEKKAQALEIGDAVA----GD---PHEPHPRDMDGEDEHFWSLSEEFADKDSEISAHQLKRV 559

  Fly   623 LDHSMRDDLPKPVVFNRFSNNMAFETQAAGPGDDGAGACGLLSLICGPFLKGTPFEEQLGMNDQS 687
            |:                                     ||||
Mouse   560 LN-------------------------------------GLLS---------------------- 565

  Fly   688 NKRLIGDNPADGGPVTANAIVDETHGFSKDVCRSMVAMLDADKSGKLGFEEFETLLSEIAKWKAI 752
             ||.  |...|              ||:.:.||.|:::||.|.:|.|...||:||..:|.|:..|
Mouse   566 -KRT--DMKFD--------------GFNINTCREMISLLDGDGTGSLRPVEFKTLWLKICKYLEI 613

  Fly   753 FKVYDVENTGRVSGFQLREALNSAGYHLNNRVLNVLGHRYGSRDGKIAFDDFIMCAVKIKTYIDI 817
            ::..|....|.:...::|.||..||:.|||:|...:..||......:.||.|:.|.::::....:
Mouse   614 YQEMDHSRAGTIDAHEMRTALKKAGFTLNNQVQQTIATRYACSKLGVDFDGFVACMIRLEILFKL 678

  Fly   818 FKERDTEKNETATFTLEEWI 837
            |:..|.::|.....:|.||:
Mouse   679 FRLLDKDQNGIVQLSLAEWL 698

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:279042	173/304 (57%)
		Calpain_III	418..565	CDD:279416	74/158 (47%)
		EFh	719..765	CDD:238008	17/45 (38%)
Capn8	NP_570960.2	Peptidase_C2	46..342	CDD:279042	173/304 (57%)
		Domain III	355..512		75/162 (46%)
		Calpain_III	356..509	CDD:279416	74/158 (47%)
		Linker. /evidence=ECO:0000250	513..531		7/24 (29%)
		Domain IV. /evidence=ECO:0000250	532..703		58/242 (24%)
		EFh	580..635	CDD:298682	19/54 (35%)
		PTZ00183	611..>692	CDD:185503	21/80 (26%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	357	1.000	Domainoid score	I968
eggNOG	1	0.900	-	-		E1_KOG0045
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	640	1.000	Inparanoid score	I814
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG48339
OrthoDB	1	1.010	-	-		D704215at2759
OrthoFinder	1	1.000	-	-		FOG0000071
OrthoInspector	1	1.000	-	-		mtm8819
orthoMCL	1	0.900	-	-		OOG6_100404
Panther	1	1.100	-	-	O	PTHR10183
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2435
SonicParanoid	1	1.000	-	-		X74
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	13	12.910