Protein Alignment CalpA and Capn7

DIOPT Version :10

• Sequence 1: NP_001286613.1
• Gene: CalpA / 37232
• FlyBaseID: FBgn0012051
• Length: 843
• Species: Drosophila melanogaster
• Sequence 2: NP_033926.1
• Gene: Capn7 / 12339
• MGIID: 1338030
• Length: 813
• Species: Mus musculus
• Alignment Length: 752
• Identity: 157/752 - (20%)
• Similarity: 258/752 - (34%)
• Gaps: 282/752 - (37%)

  Fly     4 LRGFLRQAGQEFLNAAGEAAMGAAKDVVGSVINEIFIK-KEADTKRVLPSIK-------NMRVQP 60
            |:..|:|..::.|:.|         :.:...:.:.|.| |.|:.|...|.::       |..|:.
Mouse   132 LQNKLKQLARQALDRA---------EALSEPLTKPFCKLKSANMKTKTPPVRTHFPLGPNPFVEK 187

  Fly    61 LQS----QSNSTLYYGNLGEKSSSLGPYSEVQDYETI--------------LNSCLASGSLFEDP 107
            .|:    ||..........|:...|...|::...|.:              :..|...|.|   |
Mouse   188 PQAFISPQSCDAQGQKYTAEEIEVLRTTSKINGVEYVPFMSVDLRERFAYPMPFCDRLGKL---P 249

  Fly   108 LFPASNESLQFSRRPDRHIEWLRPHEIAENPQFFVEGYSRFDVQQGELGDCWLLAATANLTQESN 172
            |.|  .:...||:       |:||.::..||. .:...|.|.::|..:.||..:|:.|.......
Mouse   250 LSP--KQKTTFSK-------WVRPEDLTNNPT-MIYTVSSFSIKQTIVSDCSFVASLAISAAYER 304

  Fly   173 LFFR------VIPAEQSFEENY--AGIFHFRFWQYGKWVDVIIDDRLPT-YNGELMYMHSTEKNE 228
            .|.:      :.|..:..|..|  .|.:..:....|....|||||:||. :.|||:..:|..|:|
Mouse   305 RFNKKLITSIIYPQNKDGEPEYNPCGKYMVKLHLNGVPRKVIIDDQLPVDHKGELLCSYSNNKSE 369

  Fly   229 FWSALLEKAYAKLHGSYEALKGGSTCEAMEDFTGGVSEWYDLKE-----APGNLFTILQKAAERN 288
            .|.:|:||||.|:.|.|: ..|.::...:...||.:.|...:..     :..|.|.:|.:...:.
Mouse   370 LWVSLIEKAYMKVMGGYD-FPGSNSNIDLHALTGWIPERIAMHSDSQTFSKDNSFRMLYQRFHKG 433

  Fly   289 SMMGCSIEPDPNV-TEAETPQ-GLIRGHAYSITKVCLIDIVTPNRQGK-IPMIRMRNPWGNEAEW 350
            .::   |.....| ||||..: ||:..|||::     :||    |:.| :..|:::||| :...|
Mouse   434 DVL---ITASTGVMTEAEGEKWGLVPTHAYAV-----LDI----REFKGLRFIQLKNPW-SHLRW 485

  Fly   351 NGPWSDSSPEWRYIPEEQKAEIGLTFD-------RDGEFWMSFQDFLNHFDRV------------ 396
            .|.:|::..: .:.||.||.   |.||       .:|.||:|:.|...::|.|
Mouse   486 KGRYSENDVK-NWTPELQKY---LNFDPRTAQKIDNGIFWISWDDLCQYYDVVYLSWNPALFKES 546

  Fly   397 ------------------EICNLSPDSLTEDQ--------------------------------- 410
                              .:.| :|....|.|
Mouse   547 TCIHSTWDAKQGPVKDAYSLAN-NPQYKLEVQCPQGGAAVWVLLSRHITDKDDFANNREFITMVV 610

  Fly   411 -QNSGKRKW-----------------------------------EMSMYE--------------- 424
             :..||:.:                                   .:|.||
Mouse   611 YKTDGKKVYYPADPPPYIDGIRINSPHYLTKIKLTTPGTHTFTLVVSQYEKQNTIHYTVRVYSAC 675

  Fly   425 ------------------GEWTPGVTAGGCRNFLDTFWHNPQYIITLVDPDEEDEEGQCTVIVAL 471
                              |:|: |.:||||.||.:|..:||.|...:      |:.|.     .|
Mouse   676 SFTFSKIPSPYTLSKRINGKWS-GQSAGGCGNFQETHKNNPIYQFHI------DKTGP-----LL 728

  Fly   472 MQKNRRSKRNMGMECLTI---------GFAIYSLND-------RELENRPQGLNFFRYKSSVGRS 520
            ::.....:.::|.|.:.:         ||...|..|       .||||
Mouse   729 IELRGPRQYSVGFEVVAVSIMGDPGPHGFQRKSSGDYRCGFCYLELEN----------------- 776

  Fly   521 PHFINTREVCARFKLPPGHYLIVPSTFDPNEEGEFII 557
                          :|.|.:.|:||||.|.:||.|.:
Mouse   777 --------------IPAGIFNIIPSTFLPKQEGPFFL 799

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
CalpA	NP_001286613.1	Peptidase_C2	104..400	CDD:459889	88/349 (25%)
		Calpain_III	416..569	CDD:238132	40/226 (18%)
		EFh_PEF_CalpA_B	600..843	CDD:320071
		EF-hand motif	600..627	CDD:320071
		EF-hand motif	717..747	CDD:320071
		EF-hand motif	748..778	CDD:320071
		EF-hand motif	784..812	CDD:320071
		EF-hand motif	813..843	CDD:320071
Capn7	NP_033926.1	MIT_calpain7_1	5..76	CDD:239144
		MIT	85..157	CDD:469712	5/33 (15%)
		CysPc	214..538	CDD:238004	93/354 (26%)
		Domain III	541..701		12/161 (7%)
		calpain_III	687..810	CDD:214786	37/156 (24%)
		Domain N	702..813		33/140 (24%)