DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT1A and Htr1f

DIOPT Version :9

Sequence 1:	NP_001356890.1	Gene:	5-HT1A / 37196	FlyBaseID:	FBgn0004168	Length:	846	Species:	Drosophila melanogaster
Sequence 2:	NP_068629.2	Gene:	Htr1f / 60448	RGDID:	71083	Length:	366	Species:	Rattus norvegicus

Alignment Length:	643	Identity:	156/643 - (24%)
Similarity:	232/643 - (36%)	Gaps:	286/643 - (44%)

- Green bases have known domain annotations that are detailed below.

  Fly   180 LEGNDSSLFGEMLANRSGQLDLINGTGGLNVTTSKVAEDDFTQLLRMAVTSVLLGLMILVTIIGN 244
            |..:|.:|..|.|.||               ..||:           .|:..|.||.::.|.| |
  Rat     4 LNSSDQNLTSEELLNR---------------MPSKI-----------LVSLTLSGLALMTTTI-N 41

  Fly   245 VFVIAAIILERNLQNVANYLVASLAVADLFVACLVMPLGAVYEISQGWILGPELCDIWTSCDVLC 309
            ..||.|||:.|.|.:.||||:.||||.|..||.||||...||.:|:.||:|..|||:|.|.|::|
  Rat    42 SLVITAIIVTRKLHHPANYLICSLAVTDFLVAVLVMPFSIVYIVSESWIMGQGLCDLWLSVDIIC 106

  Fly   310 CTASILHLVAIAVDRYWAVTN-IDYIHSRTSNRVFMMIFCVWTAAVIVSLAPQFGWKDPDYLQRI 373
            |:.|||||.|||:|||.|:|: ::|...||.....:.|..||..:|.:|:.|.| |:   :....
  Rat   107 CSCSILHLSAIALDRYRAITDAVEYARKRTPRHAGITITTVWVISVFISMPPLF-WR---HQGNS 167

  Fly   374 EQQKCMVSQD-VSYQVFATCCTFYVPLLVILALYWKIYQTARKRIHRRRPRPVDAAVNNNQPDGG 437
            ...:|::..| :...:::|...||:||::||.||:|||:.||...|:|:
  Rat   168 RDDQCIIKHDHIVSTIYSTFGAFYIPLVLILILYYKIYRAARTLYHKRQ---------------- 216

  Fly   438 AATDTKLHRLRLRLGRFSTAKSKTGSAVGVSGPASGGRALGLVDGNSTNTVNTVEDTEFSSSNVD 502

  Rat   217 ----------------------------------------------------------------- 216

  Fly   503 SKSRAGVEAPSTSGNQIATVSHLVALAKQQGKSTAKSSAAVNGMAPSGRQEDDGQRPEHGEQEDR 567
                               .|.::                                        :
  Rat   217 -------------------ASRMI----------------------------------------K 222

  Fly   568 EELEDQDEQVGPQPTTATSAMTAAGTNESEDQCKANGVEVLEDPQLQQQLEQVQQLQKSVKSGGG 632
            |||                                ||..:||..            :||:|
  Rat   223 EEL--------------------------------NGQVLLESG------------EKSIK---- 239

  Fly   633 GGASTSNATTITSISALSPQTPTSQGVGIAAAAAGPMTAKTSTLTSCNQSHPLCGTANESPSTPE 697
                                                 ...||.:...:.|.|.........:...
  Rat   240 -------------------------------------LVSTSYMLEKSLSDPSTDFDRIHSTVKS 267

  Fly   698 PRSRQPTTPQQQPHQQAHQQQQQQQQLSSIANPMQKVNKRKETLEAKRERKAAKTLAIITGAFVV 762
            |||           :..|::..::|::|                 ..||||||.||.:|.||||:
  Rat   268 PRS-----------ELKHEKSWRRQKIS-----------------GTRERKAATTLGLILGAFVI 304

  Fly   763 CWLPFFVMALTMPLCAACQISDSVASLFLWLGYFNSTLNPVIYTIFSPEFRQAFKRIL 820
            |||||||..|.:.:|..|:||:.:::...||||.||.:||:|||||:.:|::||::::
  Rat   305 CWLPFFVKELVVNICEKCKISEEMSNFLAWLGYLNSLINPLIYTIFNEDFKKAFQKLV 362

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1A	NP_001356890.1	7tmA_5-HT1A_invertebrates	227..>413	CDD:320454	81/187 (43%)
		TM helix 1	228..254	CDD:320454	12/25 (48%)
		TM helix 2	261..287	CDD:320454	16/25 (64%)
		TM helix 3	299..329	CDD:320454	19/29 (66%)
		TM helix 4	340..362	CDD:320454	6/21 (29%)
		TM helix 5	383..412	CDD:320454	13/29 (45%)
		7tm_GPCRs	<743..816	CDD:355774	39/72 (54%)
		TM helix 6	747..772	CDD:341315	18/24 (75%)
		TM helix 7	784..809	CDD:341315	12/24 (50%)
Htr1f	NP_068629.2	7tm_1	41..347	CDD:278431	133/562 (24%)
		7tm_4	41..>222	CDD:304433	80/324 (25%)
		Agonist binding. /evidence=ECO:0000250	99..108		4/8 (50%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250	120..122		1/1 (100%)
		Agonist binding. /evidence=ECO:0000250	306..310		3/3 (100%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	343..347		2/3 (67%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353597
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D310620at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X366
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	5	4.850