Protein Alignment 5-HT1A and HTR4

DIOPT Version :10

• Sequence 1: NP_001356890.1
• Gene: 5-HT1A / 37196
• FlyBaseID: FBgn0004168
• Length: 846
• Species: Drosophila melanogaster
• Sequence 2: NP_001035263.1
• Gene: HTR4 / 3360
• HGNCID: 5299
• Length: 428
• Species: Homo sapiens
• Alignment Length: 618
• Identity: 122/618 - (19%)
• Similarity: 180/618 - (29%)
• Gaps: 313/618 - (50%)

  Fly   216 AEDDFTQLLRMAVTSVLLGLMILVTIIGNVFVIAAIILERNLQNV-ANYLVASLAVADLFVACLV 279
            :|:.|..:.:: |....|..:||:.|:||:.|:.|:..:|.|:.: .||.:.|||.|||.|:.||
Human    10 SEEGFGSVEKV-VLLTFLSTVILMAILGNLLVMVAVCWDRQLRKIKTNYFIVSLAFADLLVSVLV 73

  Fly   280 MPLGAVYEISQGWILGPELCDIWTSCDVLCCTASILHLVAIAVDRYWAV--TNIDYIHSRTSNRV 342
            ||.||:..:...||.|...|.:.||.|||..||||.||..|::|||:|:  ..:.|.:..|..|:
Human    74 MPFGAIELVQDIWIYGEVFCLVRTSLDVLLTTASIFHLCCISLDRYYAICCQPLVYRNKMTPLRI 138

  Fly   343 FMMIFCVWTAAVIVSLAP-QFGWKDPDYLQRIEQQKCMVSQDVSYQVF-------ATC--CTFYV 397
            .:|:...|.....:|..| ..||.:...:..||::|...:.:.:|.||       .||  ..||:
Human   139 ALMLGGCWVIPTFISFLPIMQGWNNIGIIDLIEKRKFNQNSNSTYCVFMVNKPYAITCSVVAFYI 203

  Fly   398 PLLVILALYWKIYQTARKRIHRRRPRPVDAAVNNNQPDGGAATDTKLHRLRLRLGRFSTAKSKTG 462
            |.|:::..|::||.||::..|
Human   204 PFLLMVLAYYRIYVTAKEHAH-------------------------------------------- 224

  Fly   463 SAVGVSGPASGGRALGLVDGNSTNTVNTVEDTEFSSSNVDSKSRAGVEAPSTSGNQIATVSHLVA 527
                                                                             
Human   225 ----------------------------------------------------------------- 224

  Fly   528 LAKQQGKSTAKSSAAVNGMAPSGRQEDDGQRPEHGEQEDREELEDQDEQVGPQPTTATSAMTAAG 592
                                                                             
Human   225 ----------------------------------------------------------------- 224

  Fly   593 TNESEDQCKANGVEVLEDPQLQQQLEQVQQLQKSVKSGGGGGASTSNATTITSISALSPQTPTSQ 657
                                      |:|.||::
Human   225 --------------------------QIQMLQRA------------------------------- 232

  Fly   658 GVGIAAAAAGPMTAKTSTLTSCNQSHPLCGTANESPSTPEPRSRQPTTPQQQPHQQAHQQQQQQQ 722
                                         |.::||            .||.......|:.
Human   233 -----------------------------GASSES------------RPQSADQHSTHRM----- 251

  Fly   723 QLSSIANPMQKVNKRKETLEAKRERKAAKTLAIITGAFVVCWLPFFVMALTMPLCAACQISDSVA 787
                                 :.|.||||||.||.|.|.:||.||||..:..|. ....:...|.
Human   252 ---------------------RTETKAAKTLCIIMGCFCLCWAPFFVTNIVDPF-IDYTVPGQVW 294

  Fly   788 SLFLWLGYFNSTLNPVIYTIFSPEFRQAFKRIL 820
            :.||||||.||.|||.:|...:..||:||..||
Human   295 TAFLWLGYINSGLNPFLYAFLNKSFRRAFLIIL 327

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1A	NP_001356890.1	7tmA_5-HT1A_invertebrates	227..>413	CDD:320454	70/198 (35%)
		TM helix 1	228..254	CDD:320454	9/25 (36%)
		TM helix 2	261..287	CDD:320454	15/25 (60%)
		TM helix 3	299..329	CDD:320454	17/31 (55%)
		TM helix 4	340..362	CDD:320454	5/22 (23%)
		TM helix 5	383..412	CDD:320454	12/37 (32%)
		7tm_GPCRs	<743..816	CDD:475119	33/72 (46%)
		TM helix 6	750..772	CDD:410628	14/21 (67%)
		TM helix 7	784..809	CDD:410628	13/24 (54%)
HTR4	NP_001035263.1	7tmA_5-HT4	20..323	CDD:320184	116/602 (19%)
		TM helix 1	21..47	CDD:320184	9/25 (36%)
		TM helix 2	55..81	CDD:320184	15/25 (60%)
		TM helix 3	93..123	CDD:320184	17/29 (59%)
		TM helix 4	136..159	CDD:320184	5/22 (23%)
		TM helix 5	189..218	CDD:320184	9/28 (32%)
		TM helix 6	252..282	CDD:320184	17/29 (59%)
		TM helix 7	291..316	CDD:320184	13/24 (54%)