Protein Alignment 5-HT1A and HTR1B

DIOPT Version :9

• Sequence 1: NP_001356890.1
• Gene: 5-HT1A / 37196
• FlyBaseID: FBgn0004168
• Length: 846
• Species: Drosophila melanogaster
• Sequence 2: NP_000854.1
• Gene: HTR1B / 3351
• HGNCID: 5287
• Length: 390
• Species: Homo sapiens
• Alignment Length: 594
• Identity: 150/594 - (25%)
• Similarity: 216/594 - (36%)
• Gaps: 267/594 - (44%)

  Fly   231 VLLGLMILVTIIGNVFVIAAIILERNLQNVANYLVASLAVADLFVACLVMPLGAVYEISQGWILG 295
            :||.|:.|.|.:.|.||||.:...|.|...||||:|||||.||.|:.||||:..:|.::..|.||
Human    54 MLLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLG 118

  Fly   296 PELCDIWTSCDVLCCTASILHLVAIAVDRYWAVTN-IDYIHSRTSNRVFMMIFCVWTAAVIVSLA 359
            ..:||.|.|.|:.|||||||||..||:|||||:|: ::|...||..|..:||..||..::.:||
Human   119 QVVCDFWLSSDITCCTASILHLCVIALDRYWAITDAVEYSAKRTPKRAAVMIALVWVFSISISL- 182

  Fly   360 PQFGWKDPDYLQRIEQQKCMVSQD-VSYQVFATCCTFYVPLLVILALYWKIYQTARKRIHRRRPR 423
            |.|.|:.....:.:.:  |:|:.| :.|.|::|...||.|.|:::|||.:||..||.||.::.|.
Human   183 PPFFWRQAKAEEEVSE--CVVNTDHILYTVYSTVGAFYFPTLLLIALYGRIYVEARSRILKQTPN 245

  Fly   424 PVDAAVNNNQPDGGAATDTKLHRLRLRLGRFSTAKSKTGSAVGVSGPASGGRALGLVDGNSTNTV 488
                           .|..:|.|.:|
Human   246 ---------------RTGKRLTRAQL--------------------------------------- 256

  Fly   489 NTVEDTEFSSSNVDSKSRAGVEAPSTSGNQIATVSHLVALAKQQGKSTAKSSAAVNGMAPSGRQE 553
              :.|:..|:|:|.|.:....:.||.||:.:
Human   257 --ITDSPGSTSSVTSINSRVPDVPSESGSPV---------------------------------- 285

  Fly   554 DDGQRPEHGEQEDREELEDQDEQVGPQPTTATSAMTAAGTNESEDQCKANGVEV-LEDPQLQQQL 617
                                                           ..|.|:| :.|..|:
Human   286 -----------------------------------------------YVNQVKVRVSDALLE--- 300

  Fly   618 EQVQQLQKSVKSGGGGGASTSNATTITSISALSPQTPTSQGVGIAAAAAGPMTAKTSTLTSCNQS 682
                                                                             
Human   301 ----------------------------------------------------------------- 300

  Fly   683 HPLCGTANESPSTPEPRSRQPTTPQQQPHQQAHQQQQQQQQLSSIANPMQKVNKRKETLEAKRER 747
                                                                   |:.|.|.|||
Human   301 -------------------------------------------------------KKKLMAARER 310

  Fly   748 KAAKTLAIITGAFVVCWLPFFVMALTMPLCA-ACQISDSVASLFLWLGYFNSTLNPVIYTIFSPE 811
            ||.|||.||.|||:|||||||:::|.||:|. ||....::...|.||||.||.:||:|||:.:.:
Human   311 KATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHLAIFDFFTWLGYLNSLINPIIYTMSNED 375

  Fly   812 FRQAFKRIL 820
            |:|||.:::
Human   376 FKQAFHKLI 384

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1A	NP_001356890.1	7tmA_5-HT1A_invertebrates	227..>413	CDD:320454	82/183 (45%)
		TM helix 1	228..254	CDD:320454	10/22 (45%)
		TM helix 2	261..287	CDD:320454	16/25 (64%)
		TM helix 3	299..329	CDD:320454	21/29 (72%)
		TM helix 4	340..362	CDD:320454	8/21 (38%)
		TM helix 5	383..412	CDD:320454	13/29 (45%)
		7tm_GPCRs	<743..816	CDD:355774	41/73 (56%)
		TM helix 6	747..772	CDD:341315	18/24 (75%)
		TM helix 7	784..809	CDD:341315	12/24 (50%)
HTR1B	NP_000854.1	7tmA_5-HT1B_1D	46..380	CDD:320455	148/588 (25%)
		TM helix 1	51..77	CDD:320455	10/22 (45%)
		TM helix 2	84..110	CDD:320455	16/25 (64%)
		TM helix 3	122..152	CDD:320455	21/29 (72%)
		Agonist binding	125..134		4/8 (50%)
		DRY motif, important for ligand-induced conformation changes and signaling	146..148		1/1 (100%)
		TM helix 4	164..187	CDD:320455	9/23 (39%)
		TM helix 5	205..234	CDD:320455	12/28 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	259..281		7/21 (33%)
		TM helix 6	307..337	CDD:320455	21/29 (72%)
		Agonist binding	327..331		3/3 (100%)
		TM helix 7	348..373	CDD:320455	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling	365..369		2/3 (67%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165159549
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D310620at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X366
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	4	3.850