DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment 5-HT1A and Htr1b

DIOPT Version :9

Sequence 1:	NP_001356890.1	Gene:	5-HT1A / 37196	FlyBaseID:	FBgn0004168	Length:	846	Species:	Drosophila melanogaster
Sequence 2:	NP_071561.1	Gene:	Htr1b / 25075	RGDID:	2846	Length:	386	Species:	Rattus norvegicus

Alignment Length:	595	Identity:	152/595 - (25%)
Similarity:	215/595 - (36%)	Gaps:	271/595 - (45%)

- Green bases have known domain annotations that are detailed below.

  Fly   232 LLGLMILVTIIGNVFVIAAIILERNLQNVANYLVASLAVADLFVACLVMPLGAVYEISQGWILGP 296
            ||.|:.|.|.:.|.||||.:...|.|...||||:|||||.||.|:.||||:..:|.::..|.||.
  Rat    51 LLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQ 115

  Fly   297 ELCDIWTSCDVLCCTASILHLVAIAVDRYWAVTN-IDYIHSRTSNRVFMMIFCVWTAAVIVSLAP 360
            .:||.|.|.|:.||||||:||..||:|||||:|: :||...||..|..:||..||..::.:|| |
  Rat   116 VVCDFWLSSDITCCTASIMHLCVIALDRYWAITDAVDYSAKRTPKRAAIMIVLVWVFSISISL-P 179

  Fly   361 QFGWKDPDYLQRIEQQ--KCMVSQD-VSYQVFATCCTFYVPLLVILALYWKIYQTARKRIHRRRP 422
            .|.|:.    .:.|::  .|.|:.| |.|.|::|...||:|.|:::|||.:||..||.||.::.|
  Rat   180 PFFWRQ----AKAEEEVLDCFVNTDHVLYTVYSTVGAFYLPTLLLIALYGRIYVEARSRILKQTP 240

  Fly   423 RPVDAAVNNNQPDGGAATDTKLHRLRLRLGRFSTAKSKTGSAVGVSGPASGGRALGLVDGNSTNT 487
                                                :|||..:                    ..
  Rat   241 ------------------------------------NKTGKRL--------------------TR 249

  Fly   488 VNTVEDTEFSSSNVDSKSRAGVEAPSTSGNQIATVSHLVALAKQQGKSTAKSSAAVNGMAPSGRQ 552
            ...:.|:..|:|:|.|.:....|.||.||:.:
  Rat   250 AQLITDSPGSTSSVTSINSRVPEVPSESGSPV--------------------------------- 281

  Fly   553 EDDGQRPEHGEQEDREELEDQDEQVGPQPTTATSAMTAAGTNESEDQCKANGVEV-LEDPQLQQQ 616
                                                            ..|.|:| :.|..|:
  Rat   282 ------------------------------------------------YVNQVKVRVSDALLE-- 296

  Fly   617 LEQVQQLQKSVKSGGGGGASTSNATTITSISALSPQTPTSQGVGIAAAAAGPMTAKTSTLTSCNQ 681

  Rat   297 ----------------------------------------------------------------- 296

  Fly   682 SHPLCGTANESPSTPEPRSRQPTTPQQQPHQQAHQQQQQQQQLSSIANPMQKVNKRKETLEAKRE 746
                                                                    |:.|.|.||
  Rat   297 --------------------------------------------------------KKKLMAARE 305

  Fly   747 RKAAKTLAIITGAFVVCWLPFFVMALTMPLCA-ACQISDSVASLFLWLGYFNSTLNPVIYTIFSP 810
            |||.|||.||.|||:|||||||:::|.||:|. ||....::...|.||||.||.:||:|||:.:.
  Rat   306 RKATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHMAIFDFFNWLGYLNSLINPIIYTMSNE 370

  Fly   811 EFRQAFKRIL 820
            :|:|||.:::
  Rat   371 DFKQAFHKLI 380

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1A	NP_001356890.1	7tmA_5-HT1A_invertebrates	227..>413	CDD:320454	84/184 (46%)
		TM helix 1	228..254	CDD:320454	10/21 (48%)
		TM helix 2	261..287	CDD:320454	16/25 (64%)
		TM helix 3	299..329	CDD:320454	20/29 (69%)
		TM helix 4	340..362	CDD:320454	8/21 (38%)
		TM helix 5	383..412	CDD:320454	14/29 (48%)
		7tm_GPCRs	<743..816	CDD:355774	41/73 (56%)
		TM helix 6	747..772	CDD:341315	18/24 (75%)
		TM helix 7	784..809	CDD:341315	12/24 (50%)
Htr1b	NP_071561.1	7tmA_5-HT1B_1D	42..376	CDD:320455	150/589 (25%)
		TM helix 1	48..72	CDD:320455	10/20 (50%)
		TM helix 2	81..103	CDD:320455	15/21 (71%)
		TM helix 3	119..141	CDD:320455	13/21 (62%)
		Agonist binding. /evidence=ECO:0000250	121..130		4/8 (50%)
		DRY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	142..144		1/1 (100%)
		TM helix 4	164..180	CDD:320455	6/16 (38%)
		TM helix 5	202..225	CDD:320455	10/22 (45%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	255..276		7/20 (35%)
		TM helix 6	309..331	CDD:320455	15/21 (71%)
		Agonist binding. /evidence=ECO:0000250	323..327		3/3 (100%)
		TM helix 7	344..369	CDD:320455	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	361..365		2/3 (67%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C166353598
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D310620at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X366
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	4	3.850