Protein Alignment 5-HT1A and Htr1a

DIOPT Version :10

• Sequence 1: NP_001356890.1
• Gene: 5-HT1A / 37196
• FlyBaseID: FBgn0004168
• Length: 846
• Species: Drosophila melanogaster
• Sequence 2: NP_036717.2
• Gene: Htr1a / 24473
• RGDID: 2845
• Length: 422
• Species: Rattus norvegicus
• Alignment Length: 623
• Identity: 171/623 - (27%)
• Similarity: 260/623 - (41%)
• Gaps: 233/623 - (37%)

  Fly   204 GTGGLNVTTSKVAEDDFTQLLRMAVTSVLLGLMILVTIIGNVFVIAAIILERNLQNVANYLVASL 268
            |||| |||:  :::..|:.   ..:||:|||.:|...::||..|:|||.|||:||||||||:.||
  Rat    20 GTGG-NVTS--ISDVTFSY---QVITSLLLGTLIFCAVLGNACVVAAIALERSLQNVANYLIGSL 78

  Fly   269 AVADLFVACLVMPLGAVYEISQGWILGPELCDIWTSCDVLCCTASILHLVAIAVDRYWAVTN-ID 332
            ||.||.|:.||:|:.|:|::...|.||...||::.:.||||||:|||||.|||:|||||:|: ||
  Rat    79 AVTDLMVSVLVLPMAALYQVLNKWTLGQVTCDLFIALDVLCCTSSILHLCAIALDRYWAITDPID 143

  Fly   333 YIHSRTSNRVFMMIFCVWTAAVIVSLAPQFGWKDPDYLQRIEQQKCMVSQDVSYQVFATCCTFYV 397
            |::.||..|...:|...|....::|:.|..||:.|:  .|.:...|.:|:|..|.:::|...||:
  Rat   144 YVNKRTPRRAAALISLTWLIGFLISIPPMLGWRTPE--DRSDPDACTISKDHGYTIYSTFGAFYI 206

  Fly   398 PLLVILALYWKIYQTARKRIHRRRPRPVDAAVNNNQPDGGAATDTKLHRLRLRLGRFSTAKSKTG 462
            |||::|.||.:|::.||.|| |:..|.|:                               |...|
  Rat   207 PLLLMLVLYGRIFRAARFRI-RKTVRKVE-------------------------------KKGAG 239

  Fly   463 SAVGVSGPASGGRALGLVDGNSTNTVNTVEDTEFSSSNVDSKSRAGVEAPSTSGNQIATVSHLVA 527
            :::|.|
  Rat   240 TSLGTS----------------------------------------------------------- 245

  Fly   528 LAKQQGKSTAKSSAAVNGMAPSGRQEDDGQRPEHGEQEDREELEDQDEQVGPQPTTATSAMTAAG 592
                   |......::||...||    |.:|..            ::..||   |..|:.....|
  Rat   246 -------SAPPPKKSLNGQPGSG----DWRRCA------------ENRAVG---TPCTNGAVRQG 284

  Fly   593 TNESEDQCKANGVEVLEDPQLQQQLEQVQQLQKSVKSGGGGGASTSNATTITSISALSPQTPTSQ 657
            .:|:.       :||:|..::....|.:
  Rat   285 DDEAT-------LEVIEVHRVGNSKEHL------------------------------------- 305

  Fly   658 GVGIAAAAAGPMTAKTSTLTSCNQSHPLCGTANESPSTPEPRSRQPTTPQQQPHQQAHQQQQQQQ 722
                      |:.:::.:    |...|.|
  Rat   306 ----------PLPSESGS----NSYAPAC------------------------------------ 320

  Fly   723 QLSSIANPMQKVNKR----KETLEAKRERKAAKTLAIITGAFVVCWLPFFVMALTMPLCA-ACQI 782
                    :::.|:|    |..:...||||..|||.||.|.|::||||||::||.:|.|. :|.:
  Rat   321 --------LERKNERNAEAKRKMALARERKTVKTLGIIMGTFILCWLPFFIVALVLPFCENSCHM 377

  Fly   783 SDSVASLFLWLGYFNSTLNPVIYTIFSPEFRQAFKRIL 820
            ...:.::..||||.||.||||||..|:.:|:.|||:|:
  Rat   378 PALLGAIINWLGYSNSLLNPVIYAYFNKDFQNAFKKII 415

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1A	NP_001356890.1	7tmA_5-HT1A_invertebrates	227..>413	CDD:320454	88/186 (47%)
		TM helix 1	228..254	CDD:320454	12/25 (48%)
		TM helix 2	261..287	CDD:320454	15/25 (60%)
		TM helix 3	299..329	CDD:320454	21/29 (72%)
		TM helix 4	340..362	CDD:320454	5/21 (24%)
		TM helix 5	383..412	CDD:320454	12/28 (43%)
		7tm_GPCRs	<743..816	CDD:475119	36/73 (49%)
		TM helix 6	750..772	CDD:410628	13/21 (62%)
		TM helix 7	784..809	CDD:410628	12/24 (50%)
Htr1a	NP_036717.2	7tmA_5-HT1A_vertebrates	37..411	CDD:320453	159/594 (27%)
		TM helix 1	38..64	CDD:320453	12/25 (48%)
		TM helix 2	71..97	CDD:320453	15/25 (60%)
		TM helix 3	109..139	CDD:320453	21/29 (72%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	133..135		1/1 (100%)
		TM helix 4	151..173	CDD:320453	5/21 (24%)
		TM helix 5	192..221	CDD:320453	12/28 (43%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	235..261		7/91 (8%)
		TM helix 6	338..368	CDD:320453	19/29 (66%)
		TM helix 7	379..404	CDD:320453	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	396..400		3/3 (100%)