Protein Alignment 5-HT1A and Htr1b

DIOPT Version :9

• Sequence 1: NP_001356890.1
• Gene: 5-HT1A / 37196
• FlyBaseID: FBgn0004168
• Length: 846
• Species: Drosophila melanogaster
• Sequence 2: NP_034612.1
• Gene: Htr1b / 15551
• MGIID: 96274
• Length: 386
• Species: Mus musculus
• Alignment Length: 593
• Identity: 150/593 - (25%)
• Similarity: 213/593 - (35%)
• Gaps: 267/593 - (45%)

  Fly   232 LLGLMILVTIIGNVFVIAAIILERNLQNVANYLVASLAVADLFVACLVMPLGAVYEISQGWILGP 296
            ||.|:.|.|.:.|.||||.:...|.|...||||:|||||.||.|:.||||:..:|.::..|.||.
Mouse    51 LLALITLATTLSNAFVIATVYRTRKLHTPANYLIASLAVTDLLVSILVMPISTMYTVTGRWTLGQ 115

  Fly   297 ELCDIWTSCDVLCCTASILHLVAIAVDRYWAVTN-IDYIHSRTSNRVFMMIFCVWTAAVIVSLAP 360
            .:||.|.|.|:.||||||:||..||:|||||:|: ::|...||..|..:||..||..::.:|| |
Mouse   116 VVCDFWLSSDITCCTASIMHLCVIALDRYWAITDAVEYSAKRTPKRAAIMIVLVWVFSISISL-P 179

  Fly   361 QFGWKDPDYLQRIEQQKCMVSQD-VSYQVFATCCTFYVPLLVILALYWKIYQTARKRIHRRRPRP 424
            .|.|:.....:  |...|.|:.| |.|.|::|...||:|.|:::|||.:||..||.||.::.|
Mouse   180 PFFWRQAKAEE--EMLDCFVNTDHVLYTVYSTVGAFYLPTLLLIALYGRIYVEARSRILKQTP-- 240

  Fly   425 VDAAVNNNQPDGGAATDTKLHRLRLRLGRFSTAKSKTGSAVGVSGPASGGRALGLVDGNSTNTVN 489
                                              :|||..:                    ....
Mouse   241 ----------------------------------NKTGKRL--------------------TRAQ 251

  Fly   490 TVEDTEFSSSNVDSKSRAGVEAPSTSGNQIATVSHLVALAKQQGKSTAKSSAAVNGMAPSGRQED 554
            .:.|:..|:|:|.|.:....:.||.||:.:
Mouse   252 LITDSPGSTSSVTSINSRAPDVPSESGSPV----------------------------------- 281

  Fly   555 DGQRPEHGEQEDREELEDQDEQVGPQPTTATSAMTAAGTNESEDQCKANGVEV-LEDPQLQQQLE 618
                                                          ..|.|:| :.|..|:
Mouse   282 ----------------------------------------------YVNQVKVRVSDALLE---- 296

  Fly   619 QVQQLQKSVKSGGGGGASTSNATTITSISALSPQTPTSQGVGIAAAAAGPMTAKTSTLTSCNQSH 683
                                                                             
Mouse   297 ----------------------------------------------------------------- 296

  Fly   684 PLCGTANESPSTPEPRSRQPTTPQQQPHQQAHQQQQQQQQLSSIANPMQKVNKRKETLEAKRERK 748
                                                                  |:.|.|.||||
Mouse   297 ------------------------------------------------------KKKLMAARERK 307

  Fly   749 AAKTLAIITGAFVVCWLPFFVMALTMPLCA-ACQISDSVASLFLWLGYFNSTLNPVIYTIFSPEF 812
            |.|||.||.|||:|||||||:::|.||:|. ||....::...|.||||.||.:||:|||:.:.:|
Mouse   308 ATKTLGIILGAFIVCWLPFFIISLVMPICKDACWFHMAIFDFFNWLGYLNSLINPIIYTMSNEDF 372

  Fly   813 RQAFKRIL 820
            :|||.:::
Mouse   373 KQAFHKLI 380

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1A	NP_001356890.1	7tmA_5-HT1A_invertebrates	227..>413	CDD:320454	83/182 (46%)
		TM helix 1	228..254	CDD:320454	10/21 (48%)
		TM helix 2	261..287	CDD:320454	16/25 (64%)
		TM helix 3	299..329	CDD:320454	20/29 (69%)
		TM helix 4	340..362	CDD:320454	8/21 (38%)
		TM helix 5	383..412	CDD:320454	14/29 (48%)
		7tm_GPCRs	<743..816	CDD:355774	41/73 (56%)
		TM helix 6	747..772	CDD:341315	18/24 (75%)
		TM helix 7	784..809	CDD:341315	12/24 (50%)
Htr1b	NP_034612.1	7tmA_5-HT1B_1D	42..376	CDD:320455	148/587 (25%)
		TM helix 1	47..73	CDD:320455	10/21 (48%)
		TM helix 2	80..106	CDD:320455	16/25 (64%)
		TM helix 3	118..148	CDD:320455	20/29 (69%)
		Agonist binding. /evidence=ECO:0000250	121..130		4/8 (50%)
		DRY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	142..144		1/1 (100%)
		TM helix 4	160..183	CDD:320455	9/23 (39%)
		TM helix 5	201..230	CDD:320455	13/28 (46%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	255..278		7/22 (32%)
		TM helix 6	303..333	CDD:320455	21/29 (72%)
		Agonist binding. /evidence=ECO:0000250	323..327		3/3 (100%)
		TM helix 7	344..369	CDD:320455	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250	361..365		2/3 (67%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167849921
Domainoid	1	1.000	250	1.000	Domainoid score	I2104
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D310620at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X366
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.850