Protein Alignment 5-HT1B and Htr1f

DIOPT Version :10

• Sequence 1: NP_001137708.2
• Gene: 5-HT1B / 37191
• FlyBaseID: FBgn0263116
• Length: 629
• Species: Drosophila melanogaster
• Sequence 2: NP_068629.2
• Gene: Htr1f / 60448
• RGDID: 71083
• Length: 366
• Species: Rattus norvegicus
• Alignment Length: 547
• Identity: 151/547 - (27%)
• Similarity: 230/547 - (42%)
• Gaps: 198/547 - (36%)

  Fly    66 NSNTFDLVDDEQERAAVEFWLLVKM---IAMAVVLGLMILVTIIGNVFVIAAIILERNLQNVANY 127
            ||:..:|..:|         ||.:|   |.:::.|..:.|:|...|..||.|||:.|.|.:.|||
  Rat     5 NSSDQNLTSEE---------LLNRMPSKILVSLTLSGLALMTTTINSLVITAIIVTRKLHHPANY 60

  Fly   128 LVASLAVADLFVACLVMPLGAVYEISNGWILGPELCDIWTSCDVLCCTASILHLVAIAADRYWTV 192
            |:.||||.|..||.||||...||.:|..||:|..|||:|.|.|::||:.|||||.|||.|||..:
  Rat    61 LICSLAVTDFLVAVLVMPFSIVYIVSESWIMGQGLCDLWLSVDIICCSCSILHLSAIALDRYRAI 125

  Fly   193 TN-IDYNNLRTPRRVFLMIFCVWFAALIVSLAPQFGWKDPDYMKRIEEQHCMVSQD-VGYQIFAT 255
            |: ::|...||||...:.|..||..::.:|:.|.| |:   :.....:..|::..| :...|::|
  Rat   126 TDAVEYARKRTPRHAGITITTVWVISVFISMPPLF-WR---HQGNSRDDQCIIKHDHIVSTIYST 186

  Fly   256 CCTFYVPLLVILFLYWKIYIIARKRIQRRAQKSFNVTLTETDCDSAVRELKKERSKRRAERKRLE 320
            ...||:||::||.||:|||..||....:|               .|.|.:|:|.:    .:..||
  Rat   187 FGAFYIPLVLILILYYKIYRAARTLYHKR---------------QASRMIKEELN----GQVLLE 232

  Fly   321 AGERTPVDGDGTGGQLQRRTRKRMRICFGRNTNTANVVAGSEGAVARSMAAIAVDFASLAITREE 385
            :||::
  Rat   233 SGEKS------------------------------------------------------------ 237

  Fly   386 TEFSTSNYDNKSHAGTELTTVSSDADDYRTSNANEIITLSQQVAHATQHHLIASHLNAITPLAQS 450
                                                                             
  Rat   238 ----------------------------------------------------------------- 237

  Fly   451 IAMGGVGCLTTTTPSEKALSGAGTVAGAVAGGSGSGSGEEGAGTEGKNAGVGLGGVLASIANPHQ 515
                 :..::|:...||:||...|                           ....:.:::.:|..
  Rat   238 -----IKLVSTSYMLEKSLSDPST---------------------------DFDRIHSTVKSPRS 270

  Fly   516 KL----AKRRQLLEAKRERKAAQTLAIITGAFVICWLPFFVMALTMSLCKECEIHTAVASLFLWL 576
            :|    :.|||.:...||||||.||.:|.||||||||||||..|.:::|::|:|...:::...||
  Rat   271 ELKHEKSWRRQKISGTRERKAATTLGLILGAFVICWLPFFVKELVVNICEKCKISEEMSNFLAWL 335

  Fly   577 GYFNSTLNPVIYTIFNPEFRRAFKRIL 603
            ||.||.:||:||||||.:|::||::::
  Rat   336 GYLNSLINPLIYTIFNEDFKKAFQKLV 362

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1B	NP_001137708.2	TM helix 1	92..118	CDD:320454	9/25 (36%)
		TM helix 2	125..151	CDD:320454	16/25 (64%)
		TM helix 3	163..193	CDD:320454	18/29 (62%)
		TM helix 4	204..226	CDD:320454	6/21 (29%)
		TM helix 5	247..276	CDD:320454	14/29 (48%)
		7tm_GPCRs	<525..599	CDD:475119	40/73 (55%)
		TM helix 6	533..555	CDD:410628	16/21 (76%)
		TM helix 7	567..592	CDD:410628	12/24 (50%)
		7tmA_5-HT1A_invertebrates	91..>287	CDD:320454	83/197 (42%)
Htr1f	NP_068629.2	7tmA_5-HT1F	24..359	CDD:320456	143/514 (28%)
		TM helix 1	25..51	CDD:320456	9/25 (36%)
		TM helix 2	58..84	CDD:320456	16/25 (64%)
		TM helix 3	96..126	CDD:320456	18/29 (62%)
		DRY motif, important for ligand-induced conformation changes. /evidence=ECO:0000250|UniProtKB:P41595	120..122		1/1 (100%)
		TM helix 4	138..160	CDD:320456	6/21 (29%)
		TM helix 5	178..207	CDD:320456	13/28 (46%)
		TM helix 6	286..316	CDD:320456	22/29 (76%)
		TM helix 7	326..351	CDD:320456	12/24 (50%)
		NPxxY motif, important for ligand-induced conformation changes and signaling. /evidence=ECO:0000250|UniProtKB:P41595	343..347		2/3 (67%)