Protein Alignment 5-HT1B and 5-HT2A

DIOPT Version :10

• Sequence 1: NP_001137708.2
• Gene: 5-HT1B / 37191
• FlyBaseID: FBgn0263116
• Length: 629
• Species: Drosophila melanogaster
• Sequence 2: XP_061500179.1
• Gene: 5-HT2A / 1269329
• VectorBaseID: AGAMI1_010455
• Length: 825
• Species: Anopheles gambiae
• Alignment Length: 703
• Identity: 168/703 - (23%)
• Similarity: 260/703 - (36%)
• Gaps: 216/703 - (30%)

  Fly    17 PASILEIELPAIL--LNESLFIELN----GNLTQLVDTTSNLSQIVWN-------------RSIN 62
            |.|.|.::||.:|  |||..|..|:    ..||  ||...|.||.:.:             |::.
Mosquito   156 PVSELIVKLPKLLLQLNEVSFGSLDTIDTDRLT--VDGCWNASQALLDAERPTPAQPSQTARAVP 218

  Fly    63 GN--------GN--------------SNTFDLVDDEQERAAVEFWLLVKM--------------- 90
            |.        ||              |:.|:|| .......::.|:.|::
Mosquito   219 GPVQSVRLVCGNVSTESPLAAGSLFESDAFELV-SVSLLEPIQCWMNVELRNALVSPTWLNGAEH 282

  Fly    91 IAMAVVLG-------------------------LMILVTI----IGNVFVIAAIILERNLQNVAN 126
            .|:|.:.|                         |.:::.|    :||:.|..|:.|:|.||||.|
Mosquito   283 HAVAALAGWNQTQLLGELPAGGDTAPGRYEWSFLFVILFIFAGGLGNILVCLAVALDRKLQNVTN 347

  Fly   127 YLVASLAVADLFVACLVMPLGAVYEISNGWILGPELCDIWTSCDVLCCTASILHLVAIAADRYWT 191
            |.:.|||:|||.|:..||||||:......|..|...|:|:.:||||.|:|||||:..|:..||..
Mosquito   348 YFLLSLAIADLLVSLFVMPLGAIPGFLGYWPFGVTWCNIYVTCDVLACSASILHMCFISLGRYLG 412

  Fly   192 VTN-IDYNNLRTPRRVFLMIFCVWFAALIVSLAPQFGWKDPDYMKRIEEQHCM------VSQDVG 249
            :.| :...:..|.|...:.|..||..|::||       .....:..|.:.:.|      |..:..
Mosquito   413 IRNPLGSRHHSTKRLTGIKIALVWLLAMLVS-------SSITVLGMINQNNIMPGPNECVINNRA 470

  Fly   250 YQIFATCCTFYVPLLVILFLYWKIYIIARKRIQRRAQKSFNVTLTETDCDSAVRELKKERSKRRA 314
            :.:|.:...||:|:::::..|.....:.||:.:...|....             ||.:....|.|
Mosquito   471 FFVFGSLVAFYIPMVMMVVTYALTVQLLRKKARFLEQHPEG-------------ELFRRLGGRLA 522

  Fly   315 ERKRLEAGERTPVDGDGTGGQLQRRTRKRMRICFGRNTNTANVVAGSEGAVARSMAAIAVDFASL 379
            ..|.   ...:..||..|..:.....:      |..|.|........:.:...|:.::|...::|
Mosquito   523 SSKH---SNNSQSDGSSTSNKAVEMRK------FTVNNNKQPAYGQHDSSPPSSLPSVAAGHSAL 578

  Fly   380 AITREETEFST---------------------SNYDNKSHAGTELTTVSSDADDYRTSNANEIIT 423
            ......|...|                     ||..|::.||....||.    |..|...:.|
Mosquito   579 PWRWHGTTAGTGKMDSRMSSIKGSMSHPHLGYSNGGNRTMAGKRSRTVC----DQGTQTPDSI-- 637

  Fly   424 LSQQVAHATQHHLIAS---HLNAITPLAQSIAMGGVGCLTTTTPSEKALSGAGTVAGAVAGGSGS 485
                 ...|:.....|   |||::...|.:..:..:|....|..|          |.|||
Mosquito   638 -----ERETRRQKFCSFRIHLNSVPTPAINFNLKFLGSKKRTNLS----------ANAVA----- 682

  Fly   486 GSGEEGAGTEGKNAGVGLGGVLASIANPHQKLAKRRQLLEAKRERKAAQTLAIITGAFVICWLPF 550
                                                      .|:||.:.|.::...||.||.||
Mosquito   683 ------------------------------------------TEQKATKVLGLVFFTFVFCWAPF 705

  Fly   551 FVMALTMSLCKECEIHTAVASLFLWLGYFNSTLNPVIYTIFNPEFRRAFKRIL 603
            |::.:..:...|.::...:.|:.|||||.:||:||:||||||..||.||.|:|
Mosquito   706 FILNIIFAAWPEAKVPERIVSICLWLGYVSSTINPIIYTIFNKTFRAAFIRLL 758

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
5-HT1B	NP_001137708.2	TM helix 1	92..118	CDD:320454	9/54 (17%)
		TM helix 2	125..151	CDD:320454	15/25 (60%)
		TM helix 3	163..193	CDD:320454	15/29 (52%)
		TM helix 4	204..226	CDD:320454	7/21 (33%)
		TM helix 5	247..276	CDD:320454	5/28 (18%)
		7tm_GPCRs	<525..599	CDD:475119	30/73 (41%)
		TM helix 6	533..555	CDD:410628	8/21 (38%)
		TM helix 7	567..592	CDD:410628	14/24 (58%)
		7tmA_5-HT1A_invertebrates	91..>287	CDD:320454	66/231 (29%)
5-HT2A	XP_061500179.1	None