DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment GEFmeso and sif

DIOPT Version :9

Sequence 1:	NP_788403.2	Gene:	GEFmeso / 37134	FlyBaseID:	FBgn0050115	Length:	1549	Species:	Drosophila melanogaster
Sequence 2:	NP_001261446.1	Gene:	sif / 43892	FlyBaseID:	FBgn0085447	Length:	2734	Species:	Drosophila melanogaster

Alignment Length:	1539	Identity:	307/1539 - (19%)
Similarity:	484/1539 - (31%)	Gaps:	555/1539 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly   350 SEPLSPVDIVDTINFDLVAQHMERLTLADPGDLDPRAELIEAVNKTLVNKPLVRSSSAACASTIC 414
            |....|.|:|..:.   |...|....:..|...||.....|.:...:|..|....:|....|...
  Fly  1309 SSRTEPPDLVGIMR---VTDDMIDSLVCPPPPTDPPVMSEEMITGLIVPAPGWNGTSKDLYSPEA 1370

  Fly   415 -SSPLLTYARTKSLGAKASTLG------GGVPIKLPTAEQLA-ELEEANKLSAESLDRLTDIKPK 471
             |||..::....::.|:.:..|      ||:.:..||:...: |:|...| :||...|
  Fly  1371 ESSPATSFVDPAAMAAQLAVGGVGGVVVGGLGVAKPTSRTSSFEIENLLK-TAEQETR------- 1427

  Fly   472 FAARLSELARLNNRPLSSSSICSTSSSSSSGSDQLLNGKLMATSYLASVESLAESENELGDQHHP 536
                            .||...|.:||             ::|:.|.....|.::|.
  Fly  1428 ----------------KSSPTGSVTSS-------------VSTTALTPSRQLTDAEK-------- 1455

  Fly   537 PAMSVLEKTCLEIVDSERSFVEDLGQVIKGYLQDWKERACLRVDELQILFANIEEIYEFNSMLLQ 601
                 |.|..:|:||:||::|:.|..:::.||:..|....|...|:..||.||.||..|....||
  Fly  1456 -----LRKVVMELVDTERTYVKHLNNLLEHYLEPMKRETFLSNAEINALFGNIHEIVTFQRQFLQ 1515

  Fly   602 RL---INTGRDPGRIARC-------------FIDLRDGFDVYTTYCTSYPEAISLLTKLL---QA 647
            .|   ::...|..:...|             |:...:.|.:|:::|.|:.:|    .|:|   :.
  Fly  1516 NLEESLDLEPDFNKFEHCGQFRNVLFAIGSAFLYYVNHFKLYSSFCASHSKA----QKVLHPNEG 1576

  Fly   648 THT-YSLLASTQKLLQHRLPLGSYLLKPVQRILKYHLLLDSLRKHCDVKEVVEAHVI-----MRQ 706
            .|. ...||:.....||...|.|||:||:||||||.|||..:|...|.:.....|:.     |.:
  Fly  1577 NHALQEFLAARNPKQQHSSTLESYLIKPIQRILKYPLLLQQMRNLTDTRADEHVHLCEALKGMEK 1641

  Fly   707 VAHNIDQVKRKQEQ------------QSRVKELSGILDG---------WLGPELTVLGELRQEGL 750
            ||.:|::::|..|:            |...|:...:..|         ||... ..||::: :||
  Fly  1642 VAEHINEMQRIHEEYGAIFDHLFRQHQKSCKQPIDLSPGDLLYYGGVEWLNIS-DFLGKIK-KGL 1704

  Fly   751 LMEQHNKQRLVLLFATMLIITKQKEDGRLQFKTYISQNNLMLSEHLPGEPTSFYVIPFDEPRHQI 815
              |.|   .:..:|.:.::              ::.:..|...:.|.|..:.......:..|:|:
  Fly  1705 --ELH---AMCFVFKSAVV--------------FLCKERLRQKKKLMGVSSKNATNEVEIIRYQV 1750

  Fly   816 KL----------TARNRDQKRIWTQ-HIKGVMLEKLDIPMRAKELVYQLGNEEDRTPDRSTWKWS 869
            .:          :|::.|...:|.. |::..:       .|..|.||.|
  Fly  1751 LIPVTEVQVRASSAKDMDSHFLWELIHLRSQL-------QRRSEKVYVL---------------- 1792

  Fly   870 LHSGSNSTPTYLERRNACRRSEIRQ------RNSKFKRKTVANSS--------------SFDSFN 914
                ||||..:   |||..:: |||      ||.....|....||              .:...:
  Fly  1793 ----SNSTADF---RNAFLKT-IRQIIRESVRNMSIPMKNFGGSSGSVSGHSSQGMGSMGYPGNS 1849

  Fly   915 ESLEQEEESI-------SQAKPSKP---------------LARNNSLDD--------------TA 943
            ::||:.::.|       :..||.|.               |:.:...||              |.
  Fly  1850 QTLERPKQQITIVHGSHTLGKPKKKSGSQRHSAGNIDYDNLSGSQEADDLPPSVGVVHYASGHTH 1914

  Fly   944 LQKLAAAMRYRKRDAAVKEETKTPVKEPCKCDQDAKQDERIEPCSCILRDQRSRSA--------- 999
            .|::..| .:|.|...|.:.|:.....|....|..:|.::.:.    |..|....|
  Fly  1915 GQQMQPA-GFRGRSKTVGDVTEITCSSPEPHQQQQQQQQQQQQ----LMQQGHAHAHPHPHPHPR 1974

  Fly  1000 KSQSPVFSYKALKERSKSVPRIGGFSLDEEAEREREQDEDSAASLRGSKPDLSERKTKGKGFFEV 1064
            :...|......|...|..:.||     |...:.|.|:|     |.:|:   :..:.|.|
  Fly  1975 EPPPPPIRQPHLHHHSSDIERI-----DPGTKSEGEED-----SQQGT---IRPKATLG------ 2020

  Fly  1065 KQYNHKTMPKKIANLKKQRSSTTKSCS-------RFYMDLSEFDSSSSTVL-----KITESTEEL 1117
            :..||.|:            |||.:.|       ...:..|...:|...||     |.:....||
  Fly  2021 RTPNHLTL------------STTSTLSVGSTGSQARLIQSSHPPASYQPVLMKDLGKPSPDESEL 2073

  Fly  1118 RPDGEIELAQEAATL----------------DESTQDQYYSPDH-------ETELLSPAEKSKRD 1159
            ..:.|:..||.||:.                |..||.:..|..|       ::...||.:..|:|
  Fly  2074 ESESEVHAAQVAASFNLSLGSSGSLFASDARDNQTQSRTQSQIHSLHPSPRQSPRQSPRQSPKQD 2138

  Fly  1160 AQIVLDLLKNSKEFERIYNKQQ------------------KRRESPVSPVSPDIS---------- 1196
            .: |:::.::  |.||:...||                  :||:...:|...|.:
  Fly  2139 IE-VIEIFES--EAERLAASQQVAVVHQHPSDQARESGHKQRRDRDRNPTETDATARFMDKHLSD 2200

  Fly  1197 --------------------RGPILPP-----------PRPPSRSPPPLELEEQEK--------- 1221
                                |.....|           ....|.||.|..:.|..:
  Fly  2201 LEQENLTDGTCDIVAYMESLREKAFDPQDLAEDTGDHQQNDSSLSPEPQTIVENTQQTVVVDIES 2265

  Fly  1222 --KPVEEVDS--------------VSEEPIYETLLRNVHVPYKFSPVLGRAKSSQILKKRSKTAP 1270
              :|..|.||              ..||....:|..::.|..|        |..:|.|.:|...|
  Fly  2266 PSRPATESDSTGNTTGPSERSGDETEEEATTSSLASHIVVIDK--------KVDKIFKAKSPERP 2322

  Fly  1271 AASRPESDYVTLVYSPEGVLQRVGEEVVQRDSCSSSTTSTSSNGSGSTLKRDSQSTVINLLMEAA 1335
            |:::.:.....:..||:....: |...|:.....|..||.|..|     ||.|..:..:...:..
  Fly  2323 ASTKVKGGRRQIYISPDRCKSQ-GSSPVRIIKIKSPRTSLSDQG-----KRLSVCSSRDNSQDRL 2381

  Fly  1336 QEHESRPLPKNSVSG-----------------------------------------SESSLLPRA 1359
            ......|.|:.|..|                                         |..:|.||.
  Fly  2382 SAGRRTPSPRRSSEGGGILKHTTGPDPGILKPPSSPAKSKSPDRSCLKKGGPSHVCSVETLSPRV 2446

  Fly  1360 LK--SIDNLSMAFGRSNRPPERRVS-----------------------DVSEMCRQSVLHRQGSE 1399
            ..  |:|:||         |:|.::                       ||....|:..:...||.
  Fly  2447 SPRGSMDHLS---------PDRGMTSSSCLRHSPRSSFDSHGESDHNLDVPHGSRKRSMSAHGSF 2502

  Fly  1400 AVGER------------------MAHVDYADPKALFGLGVLNHS----------ERDSVFSLTSS 1436
            ..|.|                  ..|...|.|...:|.|..:.|          .|||.   |:|
  Fly  2503 ETGTRPRAQETYQYYPVYDNQTCSDHYVAAAPTGRYGGGTSSRSRLSKSLERSTSRDST---TTS 2564

  Fly  1437 SSDSMCEQQRKRSGVG-----ATFEYEQQVEDSLENDFRDSAIYSDDNEKRSGDYDVHLRRPSSP 1496
            ||     ...:..||.     ..|........|.||.|....||.                 ..|
  Fly  2565 SS-----YGHRAYGVSPDRNYVVFSSGPLRSQSAENTFSSQPIYD-----------------PLP 2607

  Fly  1497 PPPPPPLGPRPHQPPQIAPKPSKDQLIQQ-------RSGVIVCQ 1533
            ..||.||    ||||....:..:.|..||       ..|...||
  Fly  2608 RHPPQPL----HQPPMHHQQQQQQQQQQQYMSYAPEMGGYTGCQ 2647

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
GEFmeso	NP_788403.2	RhoGEF	548..713	CDD:279015	59/189 (31%)
GEFmeso	NP_788403.2	PH_PLEKHG1_G2_G3	694..839	CDD:270063	28/181 (15%)
sif	NP_001261446.1	WH1	35..144	CDD:214674
		PH1_Tiam1_2	860..985	CDD:269937
		PH	873..974	CDD:278594
		TIAM1_RBD	1141..1219	CDD:176413
		PDZ_signaling	1221..1308	CDD:238492
		RhoGEF	1456..1647	CDD:238091	60/194 (31%)
		PH2_Tiam1_2	1650..1823	CDD:269957	40/224 (18%)
		PH	1693..1812	CDD:278594	31/170 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45462288
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
	3	2.870