DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment GEFmeso and RhoGEF3

DIOPT Version :9

Sequence 1:	NP_788403.2	Gene:	GEFmeso / 37134	FlyBaseID:	FBgn0050115	Length:	1549	Species:	Drosophila melanogaster
Sequence 2:	NP_612024.4	Gene:	RhoGEF3 / 38050	FlyBaseID:	FBgn0264707	Length:	3519	Species:	Drosophila melanogaster

Alignment Length:	1475	Identity:	277/1475 - (18%)
Similarity:	466/1475 - (31%)	Gaps:	519/1475 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    19 SSTELAPSLLGIYDTLTNSFAAGGVAAAPTATSHESKASSSGDSTPKR----------------- 66
            :.|:.|.|    :|...|:|:.||.|...:.|:|.|  .|.|||...:
  Fly  2195 NETDKARS----FDFDYNNFSRGGGAKGSSRTAHTS--GSGGDSGSNQNLRVERESRSFDDEICE 2253

  Fly    67 ---SNSNCSSSSSTG-------SSTGSCSSVPTSDDQ--------SASYTLAS-------SSTHL 106
               :|:|.|:.|.:|       :.:|..|...||..:        |||...||       |||:.
  Fly  2254 AVLNNNNGSNGSVSGLRFLQPATDSGQVSMASTSSSRLRKSNSPVSASGMEASRSPQSSGSSTNN 2318

  Fly   107 GHQE------PPA--------PLVVAGLRDEP-------DGAQLSRLVQRRSQESQIHGSSS--- 147
            .||:      |.:        .|....||..|       |.......|..|::|:...|.:|
  Fly  2319 LHQQARQSGSPQSYGTRLCDHELTYEMLRKSPIMNFRRGDSGDYELPVMLRNRETINSGGNSELN 2383

  Fly   148 --------------------SIASSGSSRRGSNIRIL----SPNVHRIITHSDIQPV-----EAV 183
                                |:..|..||...|:.:.    ...::|       ||.     .:|
  Fly  2384 FMSNETRIYEHPTTVLKSQRSLRQSPGSRDDLNLEVAVGVGGDYIYR-------QPANQPRSSSV 2441

  Fly   184 SAEALKQHQQQS-----------------QRSPTQGRYIKTGHSGTPPAAGSAPKLKLSHIP--- 228
            .||.|.....:|                 |.....|. :|.|     |:|...|...|.::.
  Fly  2442 KAEFLTTATTKSCTKTLDTCDYWPRCAACQHKSVSGE-LKKG-----PSASGKPLTVLDNVQPHV 2500

  Fly   229 LSKITGKLSTQSGSELVSTFDSSN--------------------EYLNSVAFAAFE------KSK 267
            .|.|..:.:...|.:|.| .:.||                    |..:|.|..|.|      |.|
  Fly  2501 TSVIVNEDNAVPGPQLRS-LEKSNPSWTFLCSGIASICQPVQQTETDHSTAQEAAEEDIVIRKVK 2564

  Fly   268 PQDEDRTPTNKSVPGT---PFHFDGHPFQGRKLTLPRYDSQQSIKLIEMEQLAATSAQLLHAKPA 329
            |....||..::|:|..   ..:..|.|..|........|...|:.    :|:.|.|:..| ::|.
  Fly  2565 PNKSRRTGRSRSLPSVMLRRLYLAGMPKPGSNEMGTTSDCAASLS----QQIKAVSSPTL-SEPC 2624

  Fly   330 TPGTPATPTPSATKA--------------------------KEFVCSEPLSPVDIVD-------- 360
            ....|...|.|...|                          |..:...||||..:.:
  Fly  2625 LEEQPQKGTESTDVANTGSSAGSGGLGVLQKFKRTLNNFNNKNQLHISPLSPAAVGNNAPKTKTS 2689

  Fly   361 -TINFDLVAQHMERLTLADP--------------------------------------GDLDPRA 386
             |.....:|..:...|.:||                                      ||...:.
  Fly  2690 PTTTTASIAPMITTTTASDPGSEAGDTSSGKYRFGPLIWRSSKERRKTKYNRRDKCNSGDSGIQI 2754

  Fly   387 ELIEAVNKTLVNKPLVRS--------SSAACASTICSSPLLTYARTKSLGAKASTLGGGVPIKLP 443
            ||.:....:.....:.|.        ::.:|.  :..|.:....||.|  ||||::.|...:|..
  Fly  2755 ELEQDEQYSRATMAVSRQDEPRAFALTNGSCG--VALSKMRAIRRTNS--AKASSILGPFIVKTK 2815

  Fly   444 TAEQL--AELEEANKLSAESLDRLTDIKPKFAARLSELARLNNRPLSSSSICSTSSSSS------ 500
            .|:.|  .|.::|.:.:.|||...:..:|. ......:.|.:.....|.|:.|...:.|
  Fly  2816 IAKHLNIGESDKAERKAPESLPTRSLSQPN-GLESYGMGRPDLEDSDSDSVASNEEAISFYPTTY 2879

  Fly   501 --------SGSDQ---LLNGKLM------------------------------------------ 512
                    :|..|   |..|.|:
  Fly  2880 AEVLYNFTAGGPQELGLERGMLIEILRKEVGPWWFGRIKKEETNLVEDILGPELGWFPKEFVRII 2944

  Fly   513 --------------ATSYLASVESLAESE-----------------NELGDQHH--------PPA 538
                          |....|:.|:.|.:|                 ....||.:        ||.
  Fly  2945 HCPETDNFFIAHRAAADEAAAEEATARAEEGSVPVPVAAFAEDTDVTVTTDQSNVTLIVIESPPT 3009

  Fly   539 MS--------------VLEKTCL-EIVDSERSFVEDLGQVIKGYLQDWKERA-CLRVDELQILFA 587
            .|              ::.::.: |::|:|.::|:.|..:..|||....:|. ....:.::::|:
  Fly  3010 PSAPSVDFVAQPDHSTIIRRSAVRELLDTEVNYVKLLAAICDGYLPAMSKRIDIFSPNSIRLIFS 3074

  Fly   588 NIEEIYEFNSMLLQRLINTGRDPGRIARCFIDLRDGFDVYTTYCTSYPEAISLLTKLLQATHTYS 652
            ||..||:|....|:.| ..|.:..::|:.|:.:..||..|:|||.:||.|:..|       .||.
  Fly  3075 NITAIYKFQRKFLEAL-RRGIEQNQVAKVFLKMHKGFLCYSTYCNAYPRALIEL-------ETYD 3131

  Fly   653 LLASTQKLLQH--------RLPLGSYLLKPVQRILKYHLLLDSLRKHCDVKEVVEAHVIMRQVAH 709
            .:...:.:|::        .|||.::||.|||||.:|.|.|:.:.|....|...|        .:
  Fly  3132 RVKDARTILENCRESQNLAELPLSAHLLAPVQRICRYPLHLNEIIKSALEKGAKE--------GN 3188

  Fly   710 NIDQVKRKQE----QQSRVKELSGILDGWLGPELTVLGELRQEGLLMEQHNKQRLVLLFATMLII 770
            :.|..|...:    :|..|.||. |.|                       ::..:.|....|..|
  Fly  3189 DTDGAKSAGKTTDYEQLDVTELD-IPD-----------------------SQDTVNLALEAMRGI 3229

  Fly   771 TKQKEDGRLQFKTYISQNNLMLSEHLPGEPTSFYVIPFDEPRHQIKLTARNRDQKRIWTQHIKGV 835
            |:...:|:...:| |:::.... ::..|.|...:     ..|..:::.| .|.::.:|.......
  Fly  3230 TEAVNEGKRHSET-IARHQASF-QNFKGPPLHLH-----SARFFLQVDA-TRQKQNLWNSSYTLF 3286

  Fly   836 MLE------KLDIPMRAKELVY--QLGNEEDRTPDRSTWKWSLHSGSNSTPTYLERRNACRRSEI 892
            :.:      |.|| ::....:|  ::..:..|..:....|...|:..||...|.|.|:.......
  Fly  3287 LFDNQLVYCKRDI-IKRSHFIYKGRIFLDRCRVVNVRDGKMFGHTIKNSLRIYSESRDKWYDFSF 3350

  Fly   893 RQRNSKFKRKTVANSSSFDSFNESLEQEEESISQAKPSKPLARNNSLDDTALQKLAAAMRYRKRD 957
            |..|.|.:            |..:|..|.:...:|.....:        |..:     ..|.:|.
  Fly  3351 RSANRKHR------------FLSTLALERQFGGKALYVSEM--------TGFE-----YNYEERP 3390

  Fly   958 AAVKEETKTPVKEPCKCDQDAKQDERIEPCSCILRDQRSRSAKSQSPVFSYKALKERSKSVPRIG 1022
            ....:::          |.||:..|.....:         |.:|..|....|:.....:::|:
  Fly  3391 GDYSDQS----------DYDAQDFELATGVT---------SGESSLPESPAKSTSRLCETLPK-- 3434

  Fly  1023 GFSLDEEAEREREQDEDSAASLRGSKPDLSERKTKGKGFFEVKQYNHKTMPKKIANLKKQRSSTT 1087
                       :.|..|..:|...|:    ...|...|....:.:.:.....|.||....:|.|.
  Fly  3435 -----------KSQSRDGISSAENSQ----IVTTTSTGSLGRRHFGNWFRKPKSANCTPSQSPTH 3484

  Fly  1088 KSCSRFYMDLSEFDSSSSTVLKITESTEELRPDGEIELAQEAATL 1132
            |         ..||:.::    :||:..     ..:|||:.||.|
  Fly  3485 K---------PSFDADAT----LTEARV-----AAMELAEAAAAL 3511

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
GEFmeso	NP_788403.2	RhoGEF	548..713	CDD:279015	49/173 (28%)
GEFmeso	NP_788403.2	PH_PLEKHG1_G2_G3	694..839	CDD:270063	23/154 (15%)
RhoGEF3	NP_612024.4	DUF4802	<754..>790	CDD:292678
		SH3	2876..2942	CDD:214620	5/65 (8%)
		RhoGEF	3031..3235	CDD:279015	61/243 (25%)
		PH_Collybistin_ASEF	3240..3373	CDD:269931	26/153 (17%)
		PH	<3285..3363	CDD:278594	16/90 (18%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1291
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.940