Protein Alignment GEFmeso and Tiam1

DIOPT Version :10

• Sequence 1: NP_788403.2
• Gene: GEFmeso / 37134
• FlyBaseID: FBgn0050115
• Length: 1549
• Species: Drosophila melanogaster
• Sequence 2: XP_038944357.1
• Gene: Tiam1 / 304109
• RGDID: 1307455
• Length: 1609
• Species: Rattus norvegicus
• Alignment Length: 1340
• Identity: 274/1340 - (20%)
• Similarity: 452/1340 - (33%)
• Gaps: 419/1340 - (31%)

  Fly    36 NSFAAGGVAAAPTAT---SHESKASSSGDSTP--KRSNSNCSSSSSTGS---------------- 79
            :.||..|:..|.|.|   |..|.|::|..|.|  :....:.|.|||||.
  Rat   347 SEFADSGIEGATTDTDLLSRRSNATNSSYSPPTGRAFVGSDSGSSSTGDAARQGVYENFRRELEM 411

  Fly    80 STGSCSSVPT-----SDDQSASYTLAS------------------------------------SS 103
            ||.:..|:..     ||:|| |.||:|                                    |:
  Rat   412 STTNSESLEEAGSAHSDEQS-SGTLSSPGQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESA 475

  Fly   104 TH-------------------------LGHQEPPAPLV------VAGLRDEP---------DGAQ 128
            |.                         :.|...|...|      |..:.:.|         :...
  Rat   476 TRRKWKHYWVSLKGCTLFFYESDGRSGIDHNSVPKHAVWVENSIVQAVPEHPKKDFVFCLSNSLG 540

  Fly   129 LSRLVQRRSQE------SQIHGSSSSIASSGSSRRGSNIRILSPNVHRIITHSDIQPVEAVSAEA 187
            .:.|.|..||.      :.|| |:.:.|.:....:...:|:|...:.::....|:.       |.
  Rat   541 DAFLFQTTSQTELENWITAIH-SACAAAVARHHHKEDTLRLLKSEIKKLEQKIDMD-------EK 597

  Fly   188 LKQHQQQSQRSPTQGRYIKT-----------------------------------------GHSG 211
            :|:..:....|.|..:..||                                         |.:.
  Rat   598 MKKMGEMQLSSVTDAKKKKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQGGELPNPKRLLGFAS 662

  Fly   212 TPP--AAGSAPKLKLS--HIPLSKITGKLSTQSGSELVSTFDSSNEYLNSVAFAAFEKSKPQD-- 270
            .|.  |.|......:|  |..::..||::..:..::.:|...|......|..:.....||.:.
  Rat   663 RPTKVAMGRLGIFSVSSFHALVAARTGEIGVRRRTQAMSRSASKRRSRFSSLWGLDTTSKKKQGR 727

  Fly   271 --------EDRTPTNKSVPGTPFHFDGHPFQGRK---LTLPR-YDSQQSIKLIEMEQLAATSAQL 323
                    |......:|:.|.   ||.....|::   :.||. :.......:...|....:...|
  Rat   728 PTINQVFGEGTDAVKRSLEGI---FDDSVPDGKREKEVVLPSVHQHNPDCDIWVHEYFTPSWFCL 789

  Fly   324 LHAKPATPGTPATPTPSATKAKEFVCSE------------------------PLSPVDIVDTINF 364
            .:.:||.  |...|..:|....|.:|..                        |....||.:.:..
  Rat   790 PNNQPAL--TVVRPGDTARDTLELICKAHQLDHSAHYLRLKFLMENKMQFYVPQPEEDIYELLYK 852

  Fly   365 DL-----VAQ--HMERLTLADPGDLDPRAELIEAVNKTLVNKPLVRSSSAA-------------- 408
            ::     |.|  |:|:...|    .|....|:.:|::..|.:..|.|....
  Rat   853 EIEICPKVTQNIHIEKSDAA----ADNYGFLLSSVDEDGVRRLYVNSVKETGLASKKGLKAGDEI 913

  Fly   409 ------CASTICSSPLLTYARTKSLGAKASTL---GGGV------------PIKLP--------- 443
                  .|.|:.||.|..:....|||....|.   .|||            |:.|.
  Rat   914 LQINNRAAGTLNSSMLKDFLSQPSLGLLVRTYPEPEGGVELLENPPHRVDGPVDLGESPLTFLTS 978

  Fly   444 ------TAEQ--LAEL--EEANKLSAESLDRLTDIKPKFAARLSELARLNNRPLSSSSICSTSSS 498
                  ::||  .||.  ||......||.|. ||...|...:::...|         |:...|.|
  Rat   979 NPGHSLSSEQGSSAETAPEEGEGPDLESSDE-TDHSSKSTEQVTAFCR---------SLHEMSPS 1033

  Fly   499 SSSGSDQLLNGKLMATS-YLASVESLAESENELGDQHHPPAMSVLEKTCLEIVDSERSFVEDLGQ 562
            .||.|.|      .||| .||:...|::::.             |.|...|::::||::|:||..
  Rat  1034 DSSPSPQ------DATSPQLATTRQLSDADK-------------LRKVICELLETERTYVKDLNC 1079

  Fly   563 VIKGYLQDWKERACLRVDELQILFANIEEIYEFNSMLLQRLINTGR---DPGRIAR--------- 615
            :::.||:..::...|..|||.:||.|:.|:.||....|:.|.:..|   |..::.:
  Rat  1080 LMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQFKKVLF 1144

  Fly   616 ----CFIDLRDGFDVYTTYCTSYPEAISLLTKLLQATHTYSLLASTQKLLQHRLPLGSYLLKPVQ 676
                .|:...|.|.:|:.:|.|:.:...:|.|....|...:.|.:.....||...|.|||:||:|
  Rat  1145 SLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDTAFKAFLDAQNPRQQHSSTLESYLIKPIQ 1209

  Fly   677 RILKYHLLLDSLRKHCDVKEVVEAHV-----IMRQVAHNIDQVKRKQ------------EQQSRV 724
            |:|||.|||..|....|.:.....|:     .|.:||.:|:::::..            ||....
  Rat  1210 RVLKYPLLLRELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEK 1274

  Fly   725 KELSGILDG---------WLGPELTVLGELRQEGLLMEQHNKQRLVLLFATMLIITKQKEDGRLQ 780
            ||::.:..|         ||.|..: ||:.::|..|.....|..:||::          :||..|
  Rat  1275 KEVADLSMGDLLLHTSVIWLNPPAS-LGKWKKEPELAAFVFKTAVVLVY----------KDGSKQ 1328

  Fly   781 FKTYISQNNLMLSEHLPGEPTSFYVIPFDEPRHQIKLTARNRDQKRIWTQHIKGVMLEKLDIPMR 845
            .|..:..:.|.:.|.  .:|..|        ||.|.            |:.::...|...|....
  Rat  1329 KKKLVGSHRLSIYEE--WDPFRF--------RHMIP------------TEALQVRALPSADAEAN 1371

  Fly   846 AKELVYQLGNEEDRTPDRSTWKWSLH---SGSNSTPTYLERRNACRRSEIRQRNSKFKRKTVANS 907
            |...:..:.:|.:..|:|     ..|   |...|...:|:..::..|.  :.|....|.:::.::
  Rat  1372 AVCEIVHVKSESEGRPER-----VFHLCCSSPESRKDFLKSVHSILRD--KHRRQLLKTESLPSA 1429

  Fly   908 SSFDSFNE----SLEQEEESISQA--KPSKP-------LARNN-SLDDTALQKLAAAMRYRKRDA 958
            ..:..|..    :|:....::|:|  .|||.       ||||. ::|..|:          ...:
  Rat  1430 QQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAI----------SASS 1484

  Fly   959 AVKEETKTP--------VKEPCKCDQDAKQDERIEPCSCILRDQRSRSAKSQS-PVFSYKALKER 1014
            ..||..:.|        |:|.....|..:||:..|.......|:...|||..| .:...:.|:..
  Rat  1485 PEKEPQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESAKGASVDIDLQEQLQAA 1549

  Fly  1015 SKSVPRIGGFSLDEEAEREREQDEDSAAS-LRGSKPDLSE 1053
            |.|....|..:||..|.|..:..:.:|.| :.|.....||
  Rat  1550 SISQRARGRKTLDSHASRMTQLKKQAALSGINGGLESASE 1589

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
GEFmeso	NP_788403.2	RhoGEF	548..712	CDD:459876	54/184 (29%)
		PH_PLEKHG1_G2_G3	694..839	CDD:270063	33/170 (19%)
Tiam1	XP_038944357.1	PH1_Tiam1_2	450..576	CDD:269937	15/126 (12%)
		Tiam_CC_Ex	590..687	CDD:408184	13/103 (13%)
		Ubl1_cv_Nsp3_N-like	784..850	CDD:475130	11/67 (16%)
		PDZ	860..>919	CDD:214570	11/62 (18%)
		RhoGEF	1062..1251	CDD:214619	55/188 (29%)
		PH2_Tiam1_2	1253..1424	CDD:269957	40/210 (19%)