DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment GEFmeso and Pura

DIOPT Version :9

Sequence 1:	NP_788403.2	Gene:	GEFmeso / 37134	FlyBaseID:	FBgn0050115	Length:	1549	Species:	Drosophila melanogaster
Sequence 2:	NP_996016.2	Gene:	Pura / 2768948	FlyBaseID:	FBgn0035802	Length:	1904	Species:	Drosophila melanogaster

Alignment Length:	1514	Identity:	285/1514 - (18%)
Similarity:	482/1514 - (31%)	Gaps:	580/1514 - (38%)

- Green bases have known domain annotations that are detailed below.

  Fly    32 DTLTNSFAAGGVAAAPTATSHESKASSSGDS-----TPKRSNSNCSSSSSTGSSTGSC------- 84
            |::.::.:.....:..:::|..|.:||:|:.     :..:..||...:.::|:||...
  Fly   574 DSMNSTSSGNSNVSDRSSSSSSSSSSSAGEELLTSHSEAKMRSNSVGNEASGASTSPALPLYCLG 638

  Fly    85 SSVPTSD-DQSAS--------YTLASSSTHL----GHQEPPAPLVVAG--------LRDEP---- 124
            :|.|..| |:.||        ::|.:..|.|    ...|.|..|:.:|        |:.:|
  Fly   639 NSFPHIDSDEEASDSIGPGKHHSLETCETELLPSSSIAELPEKLLTSGVYLPGTRTLQGDPLVTV 703

  Fly   125 DGA-----------------QLSRLVQRRS----------QESQIHGSSSSIASSGSSRRG---- 158
            |.|                 ..|.:.:|.|          ..:.|:|..|:.......::.
  Fly   704 DAACVASAGINCYEMATLLLYYSTIPERSSCPFTTPATPGTTATINGHKSTEQQQQQQQQQQQQK 768

  Fly   159 ---------------------------SNIRILSPNVH--RIIT----HSDIQPVEAVSAEALKQ 190
                                       .::|:|:..:.  .|:.    .|.:|.......:.|:|
  Fly   769 QQPAQQTFTILIAIEKSQHLCVIDLICQSLRLLAKQIGNCEILAVCPDSSLLQLCNGQQQQKLQQ 833

  Fly   191 HQQQSQRS----PTQG------RYIKTGHSGTPPAAGSAPKLKLSHIP-LSKITG---------- 234
            .|||:..|    |:..      ::|......|..|....|...|..:| ||.:.|
  Fly   834 QQQQNNNSDHPEPSSPSPVDLVKFISVDQVTTSVAPSQLPAGLLPSVPALSTLPGISQRHHDATK 898

  Fly   235 ------------KLSTQSGSELVSTFDSSNEYLNSVAFAAFEKSKPQDEDRTPTNKSVPGTPFHF 287
                        :..:.:|..|||               |..:.:..|....||.:.:.|
  Fly   899 WREFFAQLEAFQRQCSTAGGRLVS---------------ALSEIRAADLQGLPTRRQLYG----- 943

  Fly   288 DGHPFQGRKLTLPRYDSQ-QSIKLIEMEQLAATS--AQLLHAKPATPGTPATPTPSATKAK---- 345
                 |.|.|:....||| .|::.:...|||...  |:.:.:.|.:|...:.|..|::.:.
  Fly   944 -----QHRALSRALMDSQLHSLRKMGAGQLARLQDLARAITSAPGSPRMTSQPQSSSSSSSGAFL 1003

  Fly   346 --EFVCSEPLSPVDIVDTINFDLVAQHMERLT--------------------------------- 375
              :..|.  |..|.::.. ..|..||.:|:||
  Fly  1004 NVDAACK--LRKVTLLFN-EVDRAAQRLEQLTEQRRERLRQLTRQRALEDEISEVTSWMGSDGAE 1065

  Fly   376 ---------------------------------LADPGD---------------------LDPRA 386
                                             ||...|                     ||..|
  Fly  1066 NLQKFSQLHWDNESQLRTQEQEFEKYYFIAMKHLAKGRDLHAAALKVSVLSESANNLKSALDQFA 1130

  Fly   387 ELIEAVNK-----------------------------------TLVNK-----------PLVRSS 405
            :.:|..::                                   :|:.|           |:..|:
  Fly  1131 QKLELAHERYDAAGRLLHLLTQHQRENAAREELQRLAEQLGATSLLEKHWPVMRKTHTLPVASST 1195

  Fly   406 SAACA----STICSSPLLTYARTKSLGAKASTLGGGVPIKLPTAEQLA------ELEEANKLSAE 460
            .||.|    |..|||            |..|:..||     |::...:      .||:.:::..|
  Fly  1196 PAAAAGKRVSYRCSS------------ASRSSFEGG-----PSSSNCSCWRESRNLEDMDQMEEE 1243

  Fly   461 SLDRLTDI--------KPKFA----------ARLSELARLNNRPLSSSSICSTSSSSSSGSDQLL 507
            ..::..|.        :.|.|          .|..:|||          |||..|.:....|:||
  Fly  1244 EEEQDHDCDGGDGEEQQSKIADSGLGVCDNCERNPKLAR----------ICSCQSLNEKSHDELL 1298

  Fly   508 NGKLM---ATSYLASVESLAESENELGDQHHPPAMSV------------LEKTCL----EIVDSE 553
            ..:..   :..|:..:.|..|:...|  |.|..::.:            ::||.|    |::.:|
  Fly  1299 EDECFDRPSKRYMDIMHSPMEANAHL--QCHGSSLELPKLEELSCLDPKIQKTLLLIMREMIGTE 1361

  Fly   554 RSFVEDLGQVIKGYLQDWKERACLRVD-------ELQILFANIEEIYEF-NSMLLQRLINTGRDP 610
            |.:|..|..||:.|:.:     .||.|       :..::|.|||:|:|| ||..|..|....|:|
  Fly  1362 RDYVRSLYYVIENYIDE-----LLREDIPQPLRGQRNVIFGNIEKIFEFHNSHFLGELERYERNP 1421

  Fly   611 GRIARCFIDLRDGFDVYTTYCTSYPEAISLLTKLLQATHTYSLLASTQKLLQHRLPLGSYLLKPV 675
            .::...|:::...|.:|..|..:.|::.:||::     :..|.....|..||.:|.|.|||||||
  Fly  1422 LKVGAAFLEMESKFYLYALYNKNKPKSDTLLSE-----YGSSFFKPKQMQLQDKLDLASYLLKPV 1481

  Fly   676 QRILKYHLLLDSLRKHCDVKEVVEAHVIMRQVAHNIDQVKRKQEQ---QSRVKELSGILDGWLGP 737
            ||:.||.|||..|.|.|   :.||. ..::::|.::::::|.:|.   |.|           .|.
  Fly  1482 QRMGKYALLLQQLVKAC---KGVEG-AALQEIAADVEELQRAEEMVKFQLR-----------HGN 1531

  Fly   738 ELTVLGELR-------QEGLLMEQH-----------NKQRLVLLFATMLIITKQK---EDGRLQ- 780
            :|..:..||       ::|.|:.|:           ...|.|.||..:::.:|.:   :...|.
  Fly  1532 DLLAMDSLRDCDVNVKEQGRLLRQNEFLVWQGRGGKKTLRQVFLFEELVLFSKARRFPDHKNLDI 1596

  Fly   781 --FKTYISQNNLMLSEHLPGEPTSFYV-IPFDEPRHQIKLTARNRDQKRIWTQHIKGVMLEKLDI 842
              :|..|..:::.|:.|.....|.|.: ....:|.....|...:.|.|..||:.|..::.:
  Fly  1597 YIYKNSIKTSDIGLTAHTGDSATKFEIWFRKRKPDDTWMLQCMSEDIKNAWTEEISKLLWK---- 1657

  Fly   843 PMRAKELVYQLGNEEDRTPDRSTWKWSLHSGSNSTPTYLERRNACRRSEIRQRNSKFKRKTVANS 907
              :||.      |.|.|..:.|:      .|..|.|..          :||..|::...:::
  Fly  1658 --QAKR------NREVRLAEMSS------MGIGSKPCL----------DIRPSNNQISDRSI--- 1695

  Fly   908 SSFDSFNESLEQEEESISQAKPSKPLARNNSLDDTALQKLAAAMRYRKRDAAVKEETKTPV---K 969
                                    |||:.|                           |||.   .
  Fly  1696 ------------------------PLAQLN---------------------------KTPKLRHS 1709

  Fly   970 EPCKCDQDAKQDERIEPCSCILRDQRSRSAKSQSPVFSYKALKERSKSVPRIGG----------- 1023
            ||.|       .....|.|.|    ...|..|.:...|..::...|.|....||
  Fly  1710 EPGK-------GSMRRPNSLI----SESSLSSGTSTTSGSSISGGSSSGHNDGGGRHSLHFGKLP 1763

  Fly  1024 -------------FSLDEEAEREREQDEDSAASLRGSKPDLSERKTKGKG-----FFEVKQYNHK 1070
                         ..|..|.:..:.....|...::|:........|.|.|     .|...:.|||
  Fly  1764 GAHSASSTNCSATLELINETQALKLAKSPSGGGIKGADTAADGGNTSGNGNGNGNAFRSSKRNHK 1828

  Fly  1071 TMPKKIANLKKQRSSTTKSCSRFYMDLSEFDSSSSTVLKITESTEELRPDGEIELAQEAATLDES 1135
            .....::.|..:....:..|.....:.|..:||.|:....|..            |..|:|...|
  Fly  1829 RSTTIVSQLSMESGIVSDMCVTPDQEQSAAESSRSSWSTSTTG------------ASGASTTSAS 1881

  Fly  1136 T--QDQYYSPDHETELLSP 1152
            |  ..:|.|..|..|..||
  Fly  1882 TVILRRYRSYAHAAESASP 1900

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
GEFmeso	NP_788403.2	RhoGEF	548..713	CDD:279015	57/172 (33%)
GEFmeso	NP_788403.2	PH_PLEKHG1_G2_G3	694..839	CDD:270063	33/172 (19%)
Pura	NP_996016.2	PX_domain	15..>52	CDD:295365
		RhoGEF	1353..1532	CDD:279015	62/203 (31%)
		PH_puratrophin-1	1524..1663	CDD:270062	30/161 (19%)
		PH	1562..1655	CDD:278594	19/92 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45462285
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.840