Protein Alignment GEFmeso and Tiam2

DIOPT Version :10

• Sequence 1: NP_788403.2
• Gene: GEFmeso / 37134
• FlyBaseID: FBgn0050115
• Length: 1549
• Species: Drosophila melanogaster
• Sequence 2: NP_036008.2
• Gene: Tiam2 / 24001
• MGIID: 1344338
• Length: 1715
• Species: Mus musculus
• Alignment Length: 1519
• Identity: 316/1519 - (20%)
• Similarity: 506/1519 - (33%)
• Gaps: 553/1519 - (36%)

  Fly    12 RWEDFFGSSTELAPSLLGIYDTL-TNSFAAGGVAAAPTATSHESKASSSGDSTPKRSNSNCSSSS 75
            :|:.::   ..|....|..|:|. .||....  :|...|...|.....|....||:.:..|.|:
Mouse   532 KWKQYW---VTLKGCTLLFYETYGKNSTEQN--SAPRCALFAEDSIVQSVPEHPKKEHVFCLSN- 590

  Fly    76 STGSSTGSCSSVPTSDDQSASYTL-ASSSTHLGHQEPPAPLVVAGLRDEPDG---------AQLS 130
                   ||..|         |.. |:|.|.|.:.........|.|..:..|         :|..
Mouse   591 -------SCGDV---------YLFQATSQTDLENWVTAIHSACASLFAKKHGKEDTVRLLKSQTR 639

  Fly   131 RLVQRRSQESQIHGSSS---SIASSGSSRRG--SNIRILSPNVHRIITHSDIQPVEAVSAEALKQ 190
            .|:|:...:|::...:.   |:.|...:|:.  :.||....|:.:.  |.|:..:..
Mouse   640 SLLQKIDMDSKMKKMAELQLSVVSDPKNRKAIENQIRQWEQNLEKF--HMDLFRMRC-------- 694

  Fly   191 HQQQSQRSPTQGRYIKTGHSGTPP------AAGSAP-KLKLSHIPLSKITGKLSTQSGSELVSTF 248
                         |:.:...|..|      ||.|.| ||.|..:      |.||..|...||.:.
Mouse   695 -------------YLASLQGGELPNPKSLLAATSRPSKLALGRL------GVLSVSSFHALVCSR 740

  Fly   249 DSSNEYLNSVAFAAFEKSK-----------------------------------------PQDED 272
            |.|.....:::.....|||                                         ||..
Mouse   741 DDSTLRKRTLSLTQRGKSKKGIFSSLKGLDTLARKGREKRASITQMFDSSHSHGFLGTQLPQKS- 804

  Fly   273 RTPTNKS----VPG---------------TPFHFDGHPFQGRKLTL-PRYDSQQSIKLI-EMEQL 316
             |.:||:    :.|               |..||..:  :|..:|: |.:..:..:.|: :|.||
Mouse   805 -TNSNKAHDLHLYGSAVDSALRDSMWEVQTYVHFQDN--EGVTVTIKPEHRVEDVLALVCKMRQL 866

  Fly   317 AATSAQLLHAKPATPGTPATPTPSATKAKEFVCSE----PLSPVDIVDTINFDLV---------- 367
            ..|...|...|.................:|.|..|    |||..|:..|...|:.
Mouse   867 EPTHYGLQLRKVVDKSVEWCVPALYEYMQEQVYDEIEVFPLSVYDVQLTKTGDMTDFGFAVTVQV 931

  Fly   368 --AQHMERLTLAD--PGDL-----------------DPRAEL----IEA------VNKTLVNKPL 401
              .||:.|:.::|  |..|                 :|.::|    :||      |..|||.:|
Mouse   932 DEHQHLNRIFISDVLPDSLAYGGGLRKGNEITSLNGEPVSDLDIQQMEALFSEKSVGLTLVARP- 995

  Fly   402 VRSSSAACASTICSSPLLTYAR-TKSLGAKASTLGGGVPIKLPTAEQLAELEEANKLSAESLDRL 465
            |.:....|||...|.   .::| .|||...            |...||.|         |.||..
Mouse   996 VTTRRTLCASWSDSD---LFSRDQKSLPPS------------PNQSQLLE---------EFLDNF 1036

  Fly   466 TDIKPKFAARLSELARLNNRPLSSSSICSTSSSSSSGS-DQLLNGKLMATSYLASVESLAESENE 529
                     |.:..:..:|.|..::.:   ..|.:.|: ||:.:.:.|..::| |.:.:||...:
Mouse  1037 ---------RKTATSDFSNVPEITTGL---KRSQTEGTLDQVPHREKMEQTFL-SADQIAELCRD 1088

  Fly   530 LG-----------DQHHPPAMSV---------LEKTCLEIVDSERSFVEDLGQVIKGYLQDWKER 574
            |.           :.|.||...:         |.|...|:||:|:|:|:||..:.:.||:..:..
Mouse  1089 LNNTHTNSMEAPTESHDPPPRPLARHLSDADRLRKVIQELVDTEKSYVKDLSCLFELYLEPLQNE 1153

  Fly   575 ACLRVDELQILFANIEEIYEFNSMLLQRL---INTGRD------PGRIAR-------CFIDLRDG 623
            ..|..||::.||.::.|:.||..:.|:.|   |:...|      |.:..:       .|:...|.
Mouse  1154 TFLTQDEMESLFGSLPEMLEFQKVFLETLEDAISASSDFSVLETPSQFRKLLFSLGGSFLYYADH 1218

  Fly   624 FDVYTTYCTSYPEAISLLTKLLQATHTYSLLASTQKLLQHRLPLGSYLLKPVQRILKYHLLLDSL 688
            |.:|:.:|.::.:...:|.:........:.|.:.....||...|.|||:|||||:|||.|||..|
Mouse  1219 FKLYSGFCANHIKVQRVLERAKTDKAFKAFLDARNPTKQHSSTLESYLIKPVQRVLKYPLLLKEL 1283

  Fly   689 --------RKHCDVKEVVEAHVIMRQVAHNIDQVKR------------KQEQQSRVKELSGILDG 733
                    .:|..:.|.::|   |.:||.:|:::::            ..||....||::.:..|
Mouse  1284 VSLTDHESEEHYHLTEALKA---MEKVASHINEMQKIYEDYGMVFDQLVAEQSGTEKEVTELSMG 1345

  Fly   734 ---------WLGPELTVLGELRQEGLLMEQHNKQRLVLLFATMLIITKQKEDGRLQFKTYISQNN 789
                     ||.|.|: ||:.|::        .:..|.:|...:|:              :.:.|
Mouse  1346 ELLMHSTVSWLNPFLS-LGKARKD--------IELTVFVFKRAVIL--------------VYKEN 1387

  Fly   790 LMLSEHLPGEPTSFYVIPFDEPRHQIKLTARNRDQKRIWTQHIKGVMLEKLDIPMRAKELVYQLG 854
            ..|.:.||..         ..|.|.   :|.....|..|.            ||:.|  |..:||
Mouse  1388 CKLKKKLPSN---------SRPAHN---SADLDPFKFRWL------------IPISA--LQVRLG 1426

  Fly   855 NEEDRTPDRSTWKWSLHSGSNSTPTYLERRNACRRSEIRQRNSKFKRKTVANSSSFDSFNESLEQ 919
            |... |.:.|||: .:|:                :|||..|     .:|:......||.|::
Mouse  1427 NTAG-TENNSTWE-LIHT----------------KSEIEGR-----PETIFQLCCSDSENKT--- 1465

  Fly   920 EEESISQAKPSKPLARNNSLDDTALQKLAAAMRYRKRDAAVKEETKTPVKEPCKCDQDAKQDERI 984
                 |..|..:.:.|.|               :|:.   :|.|  .|:::.|| |:......|:
Mouse  1466 -----SIVKVIRSILREN---------------FRRH---IKCE--LPLEKTCK-DRLVPLKNRV 1504

  Fly   985 EPCSCILRDQRS-----RSAKSQSPVFSYKALKERSKSVPRIGGFSLDEEAEREREQDEDSAASL 1044
             |.|..|...||     .|:.|:.|       .|.||      |.|||.:........:.|...:
Mouse  1505 -PVSAKLASSRSLKGLRTSSSSEWP-------SEPSK------GNSLDSDECSLSSGTQSSGCPV 1555

  Fly  1045 RGSKPDLSERKTKGKGFFEVKQYNHKTMPKKIANLKKQRSSTTKSCSRFYMDLSEFDSSSSTVLK 1109
            ..|:.|                       .|...|:|....          .|:||
Mouse  1556 AESRRD-----------------------SKSTELEKDAQE----------GLAEF--------- 1578

  Fly  1110 ITESTEELRPDGEIELAQEAATLDESTQDQYYSPDHETELLSPAEKSKRDAQIVLDLLKNSKEFE 1174
                     |||   |.:|:..|.:..:|.:    |..:..||.    :|.:|....||.|:
Mouse  1579 ---------PDG---LIKESDILSDEDEDFH----HPLKQGSPT----KDIEIQFQRLKISE--- 1620

  Fly  1175 RIYNKQQKRRESPVSPVSPDISRGPILPPPRPPSRSPPPLELEEQEKKPVEEVDSVSEEPIYETL 1239
                      ||.|.||.                 ..|..|..||.|                 |
Mouse  1621 ----------ESDVHPVG-----------------QQPLTESGEQPK-----------------L 1641

  Fly  1240 LRNVHVPYKFSPVLGRAKSSQ-----ILKKRSKTAPAASRPESDYVTLVYSPEGVLQRVGEEVVQ 1299
            :|.     .|.|:..:|.|::     :||.:::.....|.||:..:.|            ..|::
Mouse  1642 VRG-----HFCPIKRKANSTKRGRGTLLKAQTRHQSLDSHPETASIDL------------NLVLE 1689

  Fly  1300 RDSCSSSTTS---------TSSNG 1314
            |:....|.||         |.|:|
Mouse  1690 REFSVQSLTSVVNEEGFYETQSHG 1713

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
GEFmeso	NP_788403.2	RhoGEF	548..712	CDD:459876	54/187 (29%)
		PH_PLEKHG1_G2_G3	694..839	CDD:270063	30/165 (18%)
Tiam2	NP_036008.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..27
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	174..249
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	263..294
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	385..418
		PH1_Tiam1_2	503..629	CDD:269937	26/118 (22%)
		Tiam_CC_Ex	643..740	CDD:408184	26/125 (21%)
		RBD	832..902	CDD:128731	15/71 (21%)
		PDZ_canonical	911..994	CDD:467153	17/82 (21%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1092..1113		3/20 (15%)
		RhoGEF	1124..1313	CDD:214619	55/191 (29%)
		PH2_Tiam1_2	1315..1487	CDD:269957	51/271 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1515..1582		23/133 (17%)