Protein Alignment GEFmeso and Mcf2l

DIOPT Version :10

• Sequence 1: NP_788403.2
• Gene: GEFmeso / 37134
• FlyBaseID: FBgn0050115
• Length: 1549
• Species: Drosophila melanogaster
• Sequence 2: NP_001357978.1
• Gene: Mcf2l / 17207
• MGIID: 103263
• Length: 1178
• Species: Mus musculus
• Alignment Length: 1215
• Identity: 243/1215 - (20%)
• Similarity: 418/1215 - (34%)
• Gaps: 404/1215 - (33%)

  Fly    90 SDDQSASYTLASSSTHLGHQEPPAPLVVAGLRDEPDGAQLSRLVQRRSQESQIHGSSSSIASSGS 154
            :|.||.|..|::      |.|..|.:        .:..||:........||.....:.|.|.|.:
Mouse   232 NDVQSTSLVLSA------HTEKKAKV--------KEDLQLALKEGNSILESLREPLAESAAHSVN 282

  Fly   155 SRRGSNIRILSPNVHRIITHSDIQPVEAVSAEALKQHQQQSQRSPTQGRYIKTGHSGTPPAAGSA 219
            ..:..|    ...|.|::  :.:...||...|...:|||:.::.
Mouse   283 QDQLDN----QATVQRLL--AQLNETEAAFDEFWAKHQQKLEQC--------------------- 320

  Fly   220 PKLKLSHIPLSKITGKLSTQSGSELVSTFDSSNEYL-------NSVA--------FAAFEKSKPQ 269
              |:|.|.          .|...|:.:|.||.::.:       ||:|        ..|||:....
Mouse   321 --LQLRHF----------EQGFREVKTTLDSMSQKIAAFTDVGNSLAHVQHLLKDLTAFEEKSSV 373

  Fly   270 DEDRTPTNKSVPGTPFHFDGHPFQGRKLTLPRYDSQQSIKLIEMEQLAATSAQLLHAKPATPGTP 334
            ..|:                    .|.|:|   :.||   |||....|..|   :|.|
Mouse   374 AVDK--------------------ARALSL---EGQQ---LIENRHYAVDS---IHPK------- 402

  Fly   335 ATPTPSATKAKEFVCSEPLSPVDIVDTINFDLVAQHMERLTL-------ADPGDLDPRAELIEAV 392
                   .:..:.:|....|.|    |....|:::.:|..:|       :|.|..
Mouse   403 -------CEELQHLCDHFASEV----TRRRGLLSKSLELHSLLETSMKWSDEGIF---------- 446

  Fly   393 NKTLVNKPLVRSSSAACASTICSSPLLTYARTKSLGAKASTLGGGVPIKLPTAEQLAE------L 451
              .|.::|:.:..|..                   ||:|:         |...|:..|      :
Mouse   447 --LLASQPVDKCQSQD-------------------GAEAA---------LQEIEKFLETGAENKI 481

  Fly   452 EEANKLSAE-----SLDRLTDIKPKF-------------AARLSELARLNNRPL----------- 487
            :|.|::..|     :.|.|..::..|             .|.|.:||....||:
Mouse   482 QELNEIYKEYECILNQDLLEHVQKVFQKQESTEEMFHRRQASLKKLAAKQTRPVQPVAPRPEALT 546

  Fly   488 -----SSSSICSTSSSSSSGSDQLLNGKLMATSYLASVESLAESE----NELGDQHHPPAMSVLE 543
                 |..|..|:.:|||.|:      .|....|..:...::|..    :..|::..  ::::|.
Mouse   547 KSPSPSPGSWRSSENSSSEGN------ALRRGPYRRAKSEMSEPRQGRTSSTGEEEE--SLAILR 603

  Fly   544 KTCL-EIVDSERSFVEDLGQVIKGYLQDWKERACLRV------DELQILFANIEEIYEFNSMLLQ 601
            :..: |::|:||::||:|..|::||..:........:      ::..|||.|:||||.|::.:..
Mouse   604 RHVMNELLDTERAYVEELLCVLEGYAAEMDNPLMAHLISTGLQNKKNILFGNMEEIYHFHNRIFL 668

  Fly   602 RLINTGRD-PGRIARCFIDLRDGFDVYTTYCTSYPEAISLLTKLLQATHTYSLLASTQKLLQHRL 665
            |.:.:..| |..:.|||::..:.|.:|..||.:.|.:.||..:....    ......||.|.|:|
Mouse   669 RELESCIDCPELVGRCFLERMEEFQIYEKYCQNKPRSESLWRQCSDC----PFFQECQKKLDHKL 729

  Fly   666 PLGSYLLKPVQRILKYHLLLDSL---RKHCDVKEVVEAHVIMRQVAHNIDQVKRKQEQQSRVKEL 727
            .|.||||||||||.||.|||..:   .|||:..|                      :.|..:..:
Mouse   730 SLDSYLLKPVQRITKYQLLLKEMLKYSKHCEGAE----------------------DLQEALSSI 772

  Fly   728 SGIL---------------DGWLGPELTVLGELRQEG--LLMEQHNK--------------QRLV 761
            .|||               ||.||.    ||:|..:|  .:...|.|              ||.:
Mouse   773 LGILKAVNDSMHLIAITGYDGNLGD----LGKLLMQGSFSVWTDHKKGHTKVKELARFKPMQRHL 833

  Fly   762 LLFATMLIITKQKEDG--------RLQFKTYISQNNLMLSEHLPGEPTSFYVIPFDEPRHQIK-L 817
            .|....::..|::|:.        ...:|..::...:.::|::.|:...|.:  :...|.::. :
Mouse   834 FLHEKAVLFCKKREENGEGYEKAPSYSYKQSLNMTAVGITENVKGDTKKFEI--WYNAREEVYII 896

  Fly   818 TARNRDQKRIWTQHIKGVMLEKLDIPMRAKELVYQLGNEEDRTPDRSTWKWSLHSGSNSTPTYLE 882
            .|...:.|..|...|:.|:..:|.....|.:  ::...:....|        |.:.|:::||...
Mouse   897 QAPTPEIKAAWVNEIRKVLTSQLQACREASQ--HRALEQSHSLP--------LPTPSSTSPTKGN 951

  Fly   883 RRNACRRSEIRQRNSKFKRKTVANSSSFDSFNESLEQEEESISQAKPSKPLARNNSLDDTALQKL 947
            .||..:..:         |||  :..|.:.:          :|.:.|..|....:....::..:.
Mouse   952 TRNVKKLED---------RKT--DPLSLEGY----------VSSSLPKPPEKGKDDAVPSSTSES 995

  Fly   948 AAAMRYR---KRDAAVKEETKTPVKEPCKCDQDAKQDERIEPCSCILRDQRSRSAKSQSPVFSYK 1009
            :|..|.|   :..|.:|....:|       |:.||:.|                .||....|..:
Mouse   996 SALSRKRFTLQGLANLKASPTSP-------DKKAKRHE----------------VKSDPTPFGLR 1037

  Fly  1010 ALKERSKSV---PRIGGFSLDEEAEREREQDEDSAASLRGSKPDLSERKTKGKGFFEVKQYNHKT 1071
            ...:.|.|:   ...||:|..||.....:.:||.....:...|          |.:.|...:.|.
Mouse  1038 GWSKTSHSLEAPEEDGGWSSAEELINSSDAEEDGGVGPKKLVP----------GKYTVVMDDEKG 1092

  Fly  1072 MPKKIANLKKQRSSTTKSCSRFYMDLSEFDSSSST----VLKITESTEELRPDGEIELAQEAATL 1132
            .|..:|    .||.          |:.|.....:.    |..:|.|.|     |.:..:..:..|
Mouse  1093 GPDTLA----MRSG----------DMVEVVEEGAEGLWYVRDLTSSKE-----GWVPASSLSTLL 1138

  Fly  1133 DESTQDQYYSPDHETELLSPAEKSKRDAQIVLDLLKNSKEFERIY 1177
            .:|:..|         .||.::.|...|     :|.:|..|...|
Mouse  1139 GKSSSAQ---------CLSSSDSSPGSA-----VLSSSSSFSEGY 1169

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
GEFmeso	NP_788403.2	RhoGEF	548..712	CDD:459876	57/173 (33%)
		PH_PLEKHG1_G2_G3	694..839	CDD:270063	30/184 (16%)
Mcf2l	NP_001357978.1	SEC14	46..191	CDD:214706
		SPEC	322..524	CDD:238103	51/298 (17%)
		RhoGEF	603..780	CDD:238091	61/202 (30%)
		PH_Dbs	787..918	CDD:269934	25/136 (18%)
		SH3_DBS	1082..1136	CDD:212791	13/72 (18%)