DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment CG30116 and Apaf1

DIOPT Version :9

Sequence 1:	NP_725799.1	Gene:	CG30116 / 37126	FlyBaseID:	FBgn0028496	Length:	1922	Species:	Drosophila melanogaster
Sequence 2:	NP_001036023.1	Gene:	Apaf1 / 11783	MGIID:	1306796	Length:	1249	Species:	Mus musculus

Alignment Length:	1469	Identity:	282/1469 - (19%)
Similarity:	491/1469 - (33%)	Gaps:	478/1469 - (32%)

- Green bases have known domain annotations that are detailed below.

  Fly   212 LLRRTQIEREVSQAMA---LTSEKILAVFRERAAQCSKGDVEAAERLRKIKDELTMNLSTDN--- 270
            |..|..:|:::..:..   :.|..:|:|..|...:......:.|..|.|      |.|:.||
Mouse    10 LQHREALEKDIKTSYIMDHMISNGVLSVIEEEKVKSQATQYQRAAALIK------MILNKDNCAY 68

  Fly   271 ---HTTLVVPGSAASEAI---------DPDNEDHESYLSKFKNKVTDKLRLLIEAHITNDPDVIK 323
               :..|:..|.....|:         ....:|.:..::.|...|      |.|..:...|.:..
Mouse    69 ISFYNALLHEGYKDLAALLQSGLPLVSSSSGKDTDGGITSFVRTV------LCEGGVPQRPVIFV 127

  Fly   324 GRKKTVQEIFHEHATHLRI---------------------------LREHTDSDALVES------ 355
            .|||.|      ||...::                           :|:|    :|:|.
Mouse   128 TRKKLV------HAIQQKLWKLNGEPGWVTIYGMAGCGKSVLAAEAVRDH----SLLEGCFSGGV 182

  Fly   356 ---RVPQQLRQNLMANFRNGSRHAPYFLCGADGSGKSAILCHLYGQVGSWFGSTRVHRVIRFAK- 416
               .:.:|.:..|:...:|                    ||....|..|:  |.|:...|..||
Mouse   183 HWVSIGKQDKSGLLMKLQN--------------------LCMRLDQEESF--SQRLPLNIEEAKD 225

  Fly   417 --------ATPRSAYNLE------LLRVICQQISIIFNIPEGYLPKDASFDPLYINTWFQNLLRR 467
                    ..|||...|:      :|:....|..|:....:..: .|:...|.::......|.|
Mouse   226 RLRVLMLRKHPRSLLILDDVWDPWVLKAFDNQCQILLTTRDKSV-TDSVMGPKHVVPVESGLGR- 288

  Fly   468 VEDMGNDVLFLFI----DDL----HLL------NPLDCDIVTALSWLPTSLP--WNVQIICSSTT 516
              :.|.::|.||:    :||    |.:      :||   :|:.:..|....|  |...:
Mouse   289 --EKGLEILSLFVNMKKEDLPAEAHSIIKECKGSPL---VVSLIGALLRDFPNRWAYYL------ 342

  Fly   517 PVEQLKFTPMQRDRFKSIEYQFDLNMGDYATKLKIPPQCIPGDVSFALYVEQQFDQLERHYGRQA 581
              .||:....:|.| ||..|.::..                 |.:.::.||...:.::.:|
Mouse   343 --RQLQNKQFKRIR-KSSSYDYEAL-----------------DEAMSISVEMLREDIKDYY---- 383

  Fly   582 VGDLA---------SYITCSEYGLSETELLELLMPTDDPESLIETKNGHFSFATFKKIHREMDLL 637
             .||:         :.:.|..:.|...|:.::|....:...|...:||. ||..:
Mouse   384 -TDLSILQKDVKVPTKVLCVLWDLETEEVEDILQEFVNKSLLFCNRNGK-SFCYY---------- 436

  Fly   638 LLLHDKIMSGKVLIQWRHNYCASVAKRRYMDVQRTRSLHCELANLFFPQDEDESTLENESNRSES 702
              |||                                |..:..              .|.|||:
Mouse   437 --LHD--------------------------------LQVDFL--------------TEKNRSQ- 452

  Fly   703 KSVISLKDRDREKDSLSAVSAVSAGRKSSSTH----HNDDTSTFYNPIAADVSYSMRHVEESWHH 763
                 |:|..|:        .|:..::....|    ..:|...:||.:|..::.:..|.|
Mouse   453 -----LQDLHRK--------MVTQFQRYYQPHTLSPDQEDCMYWYNFLAYHMASANMHKE----- 499

  Fly   764 LMRSDDTTRFKQIAVC----NFDFLLAAVQTVSISYL-------RCLIEHVRCYILDRDIELIYY 817
                          :|    :.|::.|..:.|..::|       |.:::...|.:.:...|.
Mouse   500 --------------LCALMFSLDWIKAKTELVGPAHLIHEFVAYRHILDEKDCAVCENFQEF--- 547

  Fly   818 TIRKSSDVLTRDP----MQLG------SQLISWLRPISEHDDDDNSLLSMTVRSATAWCDGYAVP 872
             :..:..:|.|.|    :|||      |::....:..::.:.|       |.|....|.:...:.
Mouse   548 -LSLNGHLLGRQPFPNIVQLGLCEPETSEVYRQAKLQAKQEGD-------TGRLYLEWINKKTIK 604

  Fly   873 LLVPLTGWLPAPLPSQIRTMT--------------VSGTGCIRA--VWLAPSKQHLI-------- 913
            .|..|.          :|..|              ::..|..:.  |:.|.:.:.|:
Mouse   605 NLSRLV----------VRPHTDAVYHACFSQDGQRIASCGADKTLQVFKAETGEKLLDIKAHEDE 659

  Fly   914 ------------LATSSGD--VQQWHIMSNSLDHIFKGHTAAVTCLLVAPQSDSELLLTGSEDAT 964
                        :||.|.|  |:.|...:..|.|.:..|:..|.|.....:|:..||.|||.|..
Mouse   660 VLCCAFSSDDSYIATCSADKKVKIWDSATGKLVHTYDEHSEQVNCCHFTNKSNHLLLATGSNDFF 724

  Fly   965 VLVWHVGLRERRAHIKNAHTAPITGVAAGANNTLIISSSEDASIAITDLASGKLRHRITHHR--- 1026
            :.:|.:..:|.| :....||..:.......::.|:.|.|.|.::.:.|:.|...|..|...|
Mouse   725 LKLWDLNQKECR-NTMFGHTNSVNHCRFSPDDELLASCSADGTLRLWDVRSANERKSINVKRFFL 788

  Fly  1027 ---GPVSGIVVAGACDVLISGGLDRTICVWDLDNFTLLNTMQMTSAVLRIDISWNSVFLLALCED 1088
               .|...:.|...|            |.|..|...::  :...:.||..||..:.  |||
Mouse   789 SSEDPPEDVEVIVKC------------CSWSADGDKII--VAAKNKVLLFDIHTSG--LLA---- 833

  Fly  1089 NALYVRTLATGKELHTLKGHKSKIR--------SISIGKDSQRCV-VGCDDTRALIYDMHAGKLV 1144
                        |:||  ||.|.|:        .:::...||.|| :...|:|..:.|..
Mouse   834 ------------EIHT--GHHSTIQYCDFSPYDHLAVIALSQYCVELWNIDSRLKVADCR----- 879

  Fly  1145 RSLPPNPGPVTAVHAM----DNDDFLVTVGGNKITFYS----FRNEELYVNPYSRHPRRKRSLKR 1201
                   |.::.||.:    |...||.......|..:.    .:|..:.             ||:
Mouse   880 -------GHLSWVHGVMFSPDGSSFLTASDDQTIRVWETKKVCKNSAIV-------------LKQ 924

  Fly  1202 HAQAQRSPSTTLPPITCFDLSRDSQQMAIASGRHVHLMRINTPEYQCTLEGHTAGVSCLKFAPNG 1266
            ........:.|:  :...|..|..|.:|..:|:..:|     ||.|         |||...:|:.
Mouse   925 EIDVVFQENETM--VLAVDNIRGLQLIAGKTGQIDYL-----PEAQ---------VSCCCLSPHL 973

  Fly  1267 EFLATGSEDRLVHIWNLALGEICNSFKGHTAPVVKVVVLMDSLRVISTDRDSMLLVWMAHSGN-- 1329
            |::|.|.||..:.|..|....:.:|..||...|..:....|...:||:..||::.||...:|:
Mouse   974 EYVAFGDEDGAIKIIELPNNRVFSSGVGHKKAVRHIQFTADGKTLISSSEDSVIQVWNWQTGDYV 1038

  Fly  1330 LLQTIQGPYKSLSVTNNMRFAVSTNGDNTLKIWS-LTQEDEKYSVSHSDEITCFEISADSVHIIS 1393
            .||..|...|...:..:.|. :|.:.|.|:|:|: :|...|:....|...:....||:|:....|
Mouse  1039 FLQAHQETVKDFRLLQDSRL-LSWSFDGTVKVWNVITGRIERDFTCHQGTVLSCAISSDATKFSS 1102

  Fly  1394 GSRDMSLKVWQATGGKLSQVLVGHSDAVTCVAVSVTNKTQVLSGSKDMN--LILWDLLTGEEVHT 1456
            .|.|.:.|:|..........|.||:..|.|.|.|:..   :|..:.|.|  :.:|::..|:.:|:
Mouse  1103 TSADKTAKIWSFDLLSPLHELKGHNGCVRCSAFSLDG---ILLATGDDNGEIRIWNVSDGQLLHS 1164

  Fly  1457 LAGHLGPVIGVKVSADGSTAVSGS---------DDKTLI 1486
            .|         .:|.:..||..|.         |.|||:
Mouse  1165 CA---------PISVEEGTATHGGWVTDVCFSPDSKTLV 1194

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG30116	NP_725799.1	AAA_16	359..505	CDD:289934	34/174 (20%)
		WD40 repeat	901..937	CDD:293791	11/59 (19%)
		WD40	933..1282	CDD:238121	82/371 (22%)
		WD40 repeat	942..982	CDD:293791	12/39 (31%)
		WD40 repeat	988..1024	CDD:293791	8/35 (23%)
		WD40	1022..1484	CDD:225201	112/498 (22%)
		WD40 repeat	1029..1064	CDD:293791	5/34 (15%)
		WD40 repeat	1071..1106	CDD:293791	9/34 (26%)
		WD40 repeat	1112..1147	CDD:293791	8/43 (19%)
		WD40 repeat	1216..1252	CDD:293791	9/35 (26%)
		WD40	1248..1502	CDD:238121	66/253 (26%)
		WD40 repeat	1258..1294	CDD:293791	11/35 (31%)
		WD40 repeat	1299..1335	CDD:293791	11/37 (30%)
		WD40 repeat	1340..1374	CDD:293791	8/34 (24%)
		WD40 repeat	1379..1415	CDD:293791	8/35 (23%)
		WD40 repeat	1421..1458	CDD:293791	10/38 (26%)
		WD40 repeat	1464..1488	CDD:293791	8/32 (25%)
Apaf1	NP_001036023.1	CARD_APAF1	7..92	CDD:260034	17/87 (20%)
		NB-ARC	129..374	CDD:395745	55/309 (18%)
		APAF1_C	453..587	CDD:407760	26/164 (16%)
		WD40	607..910	CDD:238121	73/359 (20%)
		WD 1-1. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	613..652		5/38 (13%)
		WD40 repeat	623..655	CDD:293791	4/31 (13%)
		WD 1-2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	655..694		7/38 (18%)
		WD40 repeat	661..697	CDD:293791	8/35 (23%)
		WD 1-3. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	697..738		13/41 (32%)
		WD40 repeat	702..740	CDD:293791	12/38 (32%)
		WD 1-4. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	741..780		9/38 (24%)
		WD40 repeat	747..789	CDD:293791	9/41 (22%)
		WD 1-5. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	796..837		13/72 (18%)
		WD40 repeat	801..837	CDD:293791	12/67 (18%)
		WD 1-6. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	838..877		10/38 (26%)
		WD40 repeat	843..882	CDD:293791	9/50 (18%)
		WD 1-7. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	880..910		7/29 (24%)
		WD40 repeat	885..909	CDD:293791	6/23 (26%)
		Interpropeller linker	910..921		1/10 (10%)
		WD 2-1. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	922..958		8/37 (22%)
		WD 2-2. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	959..998		15/52 (29%)
		WD40	961..1235	CDD:238121	68/256 (27%)
		WD40 repeat	964..999	CDD:293791	11/34 (32%)
		WD 2-3. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	1001..1040		11/38 (29%)
		WD40 repeat	1006..1030	CDD:293791	6/23 (26%)
		WD 2-4. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	1042..1080		10/38 (26%)
		WD40 repeat	1048..1082	CDD:293791	9/34 (26%)
		WD 2-5. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	1083..1122		9/38 (24%)
		WD40 repeat	1089..1124	CDD:293791	8/34 (24%)
		WD 2-6. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	1125..1164		11/41 (27%)
		WD40 repeat	1130..1174	CDD:293791	12/55 (22%)
		WD 2-7. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	1176..1213		5/19 (26%)
		WD40 repeat	1181..1203	CDD:293791	4/14 (29%)
		WD 2-8. /evidence=ECO:0000255\|PROSITE-ProRule:PRU00221, ECO:0000269\|PubMed:21827944	1214..1249
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG4155
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.810