DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Hsf and HSF4

DIOPT Version :9

Sequence 1:	NP_001036555.1	Gene:	Hsf / 37068	FlyBaseID:	FBgn0001222	Length:	733	Species:	Drosophila melanogaster
Sequence 2:	NP_001035757.1	Gene:	HSF4 / 3299	HGNCID:	5227	Length:	492	Species:	Homo sapiens

Alignment Length:	564	Identity:	169/564 - (29%)
Similarity:	254/564 - (45%)	Gaps:	135/564 - (23%)

- Green bases have known domain annotations that are detailed below.

  Fly    44 SGVPAFLAKLWRLVDDADTNRLICWTKDGQSFVIQNQAQFAKELLPLNYKHNNMASFIRQLNMYG 108
            |.|||||.|||.||.|..|:.||.|:..|.||::.:|::||||:||..:||:|||||:|||||||
Human    15 SPVPAFLGKLWALVGDPGTDHLIRWSPSGTSFLVSDQSRFAKEVLPQYFKHSNMASFVRQLNMYG 79

  Fly   109 FHKITSIDNGG-LRFDRDEIEFSHPFFKRNSPFLLDQIKRKISNNKNGDDKGVLKPEAMSKILTD 172
            |.|:.||:.|| ||.:||.:||.||.|.|....||::::||:...: ||| |..:||.:.::|.:
Human    80 FRKVVSIEQGGLLRPERDHVEFQHPSFVRGREQLLERVRRKVPALR-GDD-GRWRPEDLGRLLGE 142

  Fly   173 VKVMRGRQDNLDSRFSAMKQENEVLWREIASLRQKHAKQQQIVNKLIQFLITIVQPSRNMSGVKR 237
            |:.:||.|::.::|...::|:||:||||:.:|||.|.:|.:::.||||.|...:|...:.:|.||
Human   143 VQALRGVQESTEARLRELRQQNEILWREVVTLRQSHGQQHRVIGKLIQCLFGPLQAGPSNAGGKR 207

  Fly   238 HVQLMIN-----------NT------------------PEID------RARTTSETESESGGGPV 267
            .:.||::           ||                  ||.:      |||           ||:
Human   208 KLSLMLDEGSSCPTPAKFNTCPLPGALLQDPYFIQSPLPETNLGLSPHRAR-----------GPI 261

  Fly   268 IHELREELLDEVMNPSPAGYTAASHYDQESVSPPAVERPRSNMSISSHNVDYSNQSVEDLLLQGN 332
            |.::.|:      :|||.|         ..:||.:..|....:::...         |.....|:
Human   262 ISDIPED------SPSPEG---------TRLSPSSDGRREKGLALLKE---------EPASPGGD 302

  Fly   333 GTAGGNILVGGAASPMAQSVSQSPAQHDVYTVTEAPDSHVQEVPNSPPYYEEQNVLTTPMVREQE 397
            |.||               ::.:|.:.| :.||..|...|..|         |.:|........|
Human   303 GEAG---------------LALAPNECD-FCVTAPPPLPVAVV---------QAILEGKGSFSPE 342

  Fly   398 QQKRQQLKENNKLRR--QAGDVILDAGDILVDSSSPKAQRTSIQHSTQPDVMVQPMIIKSEPENS 460
            ..:..|..|....|.  ..|.:.|::||     .||:        |..|.:::||.....||
Human   343 GPRNAQQPEPGDPREIPDRGPLGLESGD-----RSPE--------SLLPPMLLQPPQESVEP--- 391

  Fly   461 SGLMDLMTPANDLYSVNFISEDMPTDIFEDALLPDGVEEAAKLDQQQKFGQSTVSSGKFASNFDV 525
            :|.:|::.|:........:..||...:.:..:...|..|.|..      |.::.|.||      .
Human   392 AGPLDVLGPSLQGREWTLMDLDMELSLMQPLVPERGEPELAVK------GLNSPSPGK------D 444

  Fly   526 PTNST--LLDANQASTSKAAAKAQA----SEEEGMAVAKYSGAE 563
            ||...  |||...|....|.....|    |..|. ..|.|.|.|
Human   445 PTLGAPLLLDVQAALGGPALGLPGALTIYSTPES-RTASYLGPE 487

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Hsf	NP_001036555.1	HSF	45..148	CDD:214654	57/103 (55%)
Hsf	NP_001036555.1	Vert_HS_TF	<607..711	CDD:284062
HSF4	NP_001035757.1	HSF	16..120	CDD:214654	57/103 (55%)
		Rootletin	<110..175	CDD:373494	23/66 (35%)
		Hydrophobic repeat HR-A/B	129..203		27/73 (37%)
		Interactions with DUSP26, MAPK1 and MAPK2	245..322		25/127 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	246..285		15/64 (23%)
		Vert_HS_TF	259..>443	CDD:368967	51/254 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	337..400		18/78 (23%)
		Hydrophobic repeat HR-C	364..389		9/37 (24%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165155319
Domainoid	1	1.000	126	1.000	Domainoid score	I5413
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1154048at2759
OrthoFinder	1	1.000	-	-		FOG0000255
OrthoInspector	1	1.000	-	-		otm41758
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR10015
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2153
SonicParanoid	1	1.000	-	-		X160
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
User_Submission	0	0.000	Not matched by this tool.
	9	8.980