DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Smurf and Hecw1

DIOPT Version :9

Sequence 1:	NP_001261061.1	Gene:	Smurf / 36999	FlyBaseID:	FBgn0029006	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	XP_006516872.1	Gene:	Hecw1 / 94253	MGIID:	2444115	Length:	1605	Species:	Mus musculus

Alignment Length:	1417	Identity:	374/1417 - (26%)
Similarity:	551/1417 - (38%)	Gaps:	412/1417 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 RKDLFRLPDPFAKVQVD-----------GTGQVYSTEISKSSLDPKWNAHYDLFLGI-GDAITIT 79
            :|.:|..|||:.|:.:.           ..||...:.|..::::|.|.|.:..|:.: .|.:.|.
Mouse   219 KKGMFFNPDPYLKISIQPGKHSIFPALPHHGQERRSTIIGNTVNPIWQAEHFSFVSLPTDVLEIE 283

  Fly    80 VWNQ----RKIHKGSGFLGCVRIPAFNIQSLKGAGFQRLD--LGKLSPDDDELVRGQI------- 131
            |.::    |.|.|  .|||.:.:|...:......|.:.:.  ||:..|.|.  |.||:
Mouse   284 VKDKFAKSRPIIK--RFLGKLSMPVQRLLERHAIGDRVVSYTLGRRLPTDH--VSGQLQFRFEIT 344

  Fly   132 --------IISLLSKDGPSSGNPLAIVGPS-GDVRGPSEDDSSE---DSLPEGWEERRTDNGRVY 184
                    .|||.::...|:....:|:... |:..|....|::|   |:.||...|   .||
Mouse   345 SSIHADDEEISLSAEPESSAETQDSIMNSMVGNSNGEPSGDATEFCKDAKPESPSE---GNG--- 403

  Fly   185 YVNHATKSTQ---------------------WDRPRQPGVVGSSHATSPQQRHNTHNGNSGDRQA 228
             ||.:....|                     .:.|.:||     ....|:| |:|....|.:..|
Mouse   404 -VNSSENQNQEHAGPVEEAAGAMEARDGSNVSEAPEEPG-----ELQDPEQ-HDTQPTLSAEEVA 461

  Fly   229 PAGPTRSTTCTNLM-----------------NNGHRSRDL-----------SVTASDERRHSTEI 265
            ...|....:.::|:                 .||.|..::           .|:..::.....|:
Mouse   462 EGLPLDEDSPSSLLPEENTALGSKVEEETVPENGAREEEMQKGKDEEEEEEDVSTLEQGEPGLEL 526

  Fly   266 LSSVGKENTSPTTPVS------ATTTPGKKT------------SSSNSSSAGGRTLEQRPTNEPA 312
            ..||.|::...:.|||      |:.....:|            .|..|:..|..|.|:....|.:
Mouse   527 RVSVRKKSRPCSLPVSELETVIASACGDAETPRTHYIRIHTLLHSMPSAQRGSTTEEEDGLEEES 591

  Fly   313 TPTSST---------TSASVRLHSNDNH------VKTPKHQTNGH---------------APPES 347
            |...|:         |.|:......|..      ...|...:.||               |.|.:
Mouse   592 TLKESSEKDGLSEVDTIAADPQSMEDGESDGATLCMAPSDCSGGHFSSLSKGIGAGQDGEAHPST 656

  Fly   348 TPTSPTGQQ-------------------------------------NYVNGNAQNGSTSGNGSGQ 375
            ...|.:..|                                     :..|||.:..|.:...|..
Mouse   657 GSESDSSPQQGADHSCEGCDASCCSPSCYSTSCYSSSCYSSSCYSSSCYNGNNRFASHTRFSSVD 721

  Fly   376 AAQ-----------------------PQS---------ASNG---WTQE------DAATTT---- 395
            :|:                       |.|         ::||   |..|      :||.:|
Mouse   722 SAKISESTVFSSQEDEEEENSAFESVPDSVQSPELDPESTNGAGPWQDELAAPGGNAARSTEGLE 786

  Fly   396 SPSTTTSPPRHSQSPPTPNISPPASVTPS------------------------ANGNV------- 429
            ||....|..|..:.|...| |.|.|..||                        ::|.|
Mouse   787 SPMAGPSNRREGECPILHN-SQPISQLPSLRPEHHHYPTIDEPLPPNWEARIDSHGRVFYVDHIN 850

  Fly   430 -----HSPNANSTPAG---SGGGSRSYTAATPGQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSG 486
                 ..|:...||.|   ||...:........|..||..:.::.||.|..:.|.:....||  |
Mouse   851 RTTTWQRPSMAPTPDGMIRSGSVHQMEQLNRRYQNIQRTMATERAEEDSGNQNSEQIPDGGG--G 913

  Fly   487 GGGGGGSGQRYASAAIAAANQAARPFLDLPPGYEMRTT---QQGQVYFYHIPTGVSTWHD----- 543
            ||||..|....:.:::....:.:     |.|....:.|   |...|.|...|...:..|.
Mouse   914 GGGGSDSEAESSQSSLDLRREGS-----LSPVNSQKVTLLLQSPAVKFITNPEFFTVLHANYSAY 973

  Fly   544 ----------------PRIPRDFDTQHLTLDAIG----------PLPSGWEQRKTASGRVYFVDH 582
                            .|..|:|:......|.:.          .||.|||.:....|:.:||||
Mouse   974 RVFTSSTCLKHMILKVRRDARNFERYQHNRDLVNFINMFADTRLELPRGWEIKTDHQGKSFFVDH 1038

  Fly   583 NNRTTQFTDPRL---SGSI---------LQMIRRGTVPPTSAANAGTPAPPSATPATPSAAA--- 632
            |:|.|.|.|||:   :|.:         ||.:|..:....|..:....|...|.|.....||
Mouse  1039 NSRATTFIDPRIPLQNGRLPNHLTHRQHLQRLRSYSAGEASEVSRNRGASLLARPGHSLIAAIRS 1103

  Fly   633 -----AVP----------------------PQATPASNAT------------------------- 645
                 ::|                      .|.:.|.|.|
Mouse  1104 QHQHESLPLAYNDKIVAFLRQPNIFEMLQERQPSLARNHTLREKIHYIRTEGNHGLDKLSCDADL 1168

  Fly   646 -------------PTTLTTTTNPPHRIVP-----DLPQ---GLLEGADLLPK-YRRDLVGKLRAL 688
                         ...|.:..:|.:...|     ..||   ||...:...|. ||||...|||..
Mouse  1169 VILLSLFEEEIMSYVPLQSAFHPGYSFSPRCSPCSSPQNSPGLQRASARAPSPYRRDFEAKLRNF 1233

  Fly   689 --RTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDM-RKRLMVKFKGEEGLDYGGVAREW 750
              :.|.:......|..:|.:.|:.:.|.::..:|....|:: |.:|.:.|.|||||||.|.:||:
Mouse  1234 YRKLEAKGFGQGPGKIKLIIRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEEGLDYSGPSREF 1298

  Fly   751 LHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTT 815
            ..|||:|:.||.||||:||.:|.||:||:|.|....::|.:|.|.||.||:|:.|.:.||..||.
Mouse  1299 FFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENYLEWFRFSGRILGLALIHQYLLDAFFTR 1363

  Fly   816 PFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASI 880
            ||||.||..|..|.|:|.:|.:.|:||.||.::||:.|::.||:|....||.:...|||.|||:.
Mouse  1364 PFYKGLLKLPCDLSDLEYLDEEFHQSLQWMKDNNITDILDLTFTVNEEVFGQVTERELKSGGANT 1428

  Fly   881 PVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVND 945
            .|||:||:||::..|.:|..||:.||..||.:||.|::.|.|:..||.|||||||.|.:.||:||
Mouse  1429 QVTEKNKKEYIERMVKWRVERGVVQQTEALLRGFYEVVDSRLVSVFDARELELVIAGTAEIDLND 1493

  Fly   946 WRNNTRLKHCTNETTQVL-WFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRL 1009
            |||||..:...::...|: |||..||.:::|.|.||||||||:|.||.:||.||:||.|.   |.
Mouse  1494 WRNNTEYRGGYHDGHLVIRWFWAAVERFNNEQRLRLLQFVTGTSSVPYEGFAALRGSNGL---RR 1555

  Fly  1010 FTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061
            |.|.....:  .:||:|||||||:|||||.:|.:|.:||..|||||..|.:|
Mouse  1556 FCIEKWGKI--TSLPRAHTCFNRLDLPPYPSYSMLYEKLLTAVEETSTFGLE 1605

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_001261061.1	C2_Smurf-like	14..135	CDD:176028	32/140 (23%)
		WW	169..198	CDD:278809	8/49 (16%)
		WW	514..546	CDD:197736	8/55 (15%)
		WW	562..594	CDD:197736	16/31 (52%)
		HECTc	702..1058	CDD:238033	171/357 (48%)
		HECTc	726..1058	CDD:214523	166/333 (50%)
Hecw1	XP_006516872.1	HECW_N	66..185	CDD:374632
		C2_NEDL1-like	206..342	CDD:176073	31/126 (25%)
		Neuromodulin	378..500	CDD:369001	26/134 (19%)
		WW	829..858	CDD:366073	2/28 (7%)
		HECW1_helix	947..1013	CDD:375862	9/65 (14%)
		WW	1019..1050	CDD:197736	16/30 (53%)
		HECTc	1249..1603	CDD:238033	171/358 (48%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.810