Protein Alignment Smurf and ITCH

DIOPT Version :10

• Sequence 1: NP_523779.1
• Gene: Smurf / 36999
• FlyBaseID: FBgn0029006
• Length: 1061
• Species: Drosophila melanogaster
• Sequence 2: NP_001244066.1
• Gene: ITCH / 83737
• HGNCID: 13890
• Length: 903
• Species: Homo sapiens
• Alignment Length: 1132
• Identity: 337/1132 - (29%)
• Similarity: 483/1132 - (42%)
• Gaps: 331/1132 - (29%)

  Fly    14 KVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAITI 78
            :::||::.|:....|..:..|.|:.:|.||  ||...||...::..|||.....:.:.....:..
Human    19 QLQITVISAKLKENKKNWFGPSPYVEVTVD--GQSKKTEKCNNTNSPKWKQPLTVIVTPVSKLHF 81

  Fly    79 TVWNQRKIHKGSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDELVRGQIIISLLSKDGPSS 143
            .||:.:.: |....||...:..:  ::||....      ||.         :::::|
Human    82 RVWSHQTL-KSDVLLGTAALDIY--ETLKSNNM------KLE---------EVVVTL-------- 120

  Fly   144 GNPLAIVGPSGDVRGPSEDDSSEDSLPEG--WEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGS 206
                    ..|..:.|:|.........:|  .|.....||
Human   121 --------QLGGDKEPTETIGDLSICLDGLQLESEVVTNG------------------------- 152

  Fly   207 SHATSPQQRHNTHNGNSGDRQAPAGPTRSTTCTNLMNNG------HRSRDLSVTASDERRHSTEI 265
                                        .|||:   .||      ....:.:::|     |....
Human   153 ----------------------------ETTCS---ENGVSLCLPRLECNSAISA-----HCNLC 181

  Fly   266 LSSVGKENTSPTTPVSATTTPGKKTSSSNSSSAGGRTLEQRPTNEPATPTSSTTSASVRLHSNDN 330
            |..:.      .:|:||:...|...:|.|..  |.|:.::             |..|
Human   182 LPGLS------DSPISASRVAGFTGASQNDD--GSRSKDE-------------TRVS-------- 217

  Fly   331 HVKTPKHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDAATTT 395
                    |||...||..            |..:|...|||.|      .|.|||..:
Human   218 --------TNGSDDPEDA------------GAGENRRVSGNNS------PSLSNGGFK------- 249

  Fly   396 SPSTTTSPPRHSQSPPTPNISPPASVTPSANGNVHSPNANSTPAGSGGGSRSYTAATPGQRSQRR 460
             ||   .|||.|: ||.|....||||    ||   ||:|.|...||..||...|
Human   250 -PS---RPPRPSR-PPPPTPRRPASV----NG---SPSATSESDGSSTGSLPPT----------- 291

  Fly   461 SSRQQGEESSTRRRSSRGTRNGGTSGGGGGGGSGQRYASAAIAAANQAARP-------------- 511
                     :|...:|.|..:|........||||.|    .:....||..|
Human   292 ---------NTNTNTSEGATSGLIIPLTISGGSGPR----PLNPVTQAPLPPGWEQRVDQHGRVY 343

  Fly   512 FLD-------------LPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIP--RDFD---------- 551
            ::|             ||||:|.|....|::|:....|..:||..|.:.  |:::
Human   344 YVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQ 408

  Fly   552 --------------------TQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRLSG 596
                                :|....|.:||||.|||:|..::||||||:||.|.||:.|||..|
Human   409 GAMQQFNQRFIYGNQDLFATSQSKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQG 473

  Fly   597 SILQ------MIRRGTVPPTSAANAGTP--------APPSATPATPSAAAAVPPQATPASNATPT 647
            .:.:      ...|.||.       |.|        ......|.|..:|....||..
Human   474 QLNEKPLPEGWEMRFTVD-------GIPYFVDHNRRTTTYIDPRTGKSALDNGPQIA-------- 523

  Fly   648 TLTTTTNPPHRIVPDLPQGLLEGADLLPKYRRDLVGKLRALRTELQTM-QPQSGHCRLEVSRNEI 711
                                         |.||...|::..|...|.: .||  |.::.|:|..:
Human   524 -----------------------------YVRDFKAKVQYFRFWCQQLAMPQ--HIKITVTRKTL 557

  Fly   712 FEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTL 776
            ||:|::.||....:|:|:||.|.|.||||||||||||||..|||.|:|||.|.||:|:..|:|.|
Human   558 FEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCL 622

  Fly   777 QINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRS 841
            ||||.|.:|||||.||.|:||.:.:|:|||..:|.||:.||||::||||:.|.|:|.:||:.:.|
Human   623 QINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNS 687

  Fly   842 LTWMLESNISGI-IESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQ 905
            |.|:.|:||... :|..|||:....|.:..|:|||.|.:|.||||||.||:::...:|..||:|:
Human   688 LIWVKENNIEECDLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEE 752

  Fly   906 QFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVE 970
            |..|..:||.|::|...|:.||.:|||:::.|:..||:|||:.:...:|....:.|::||||.|:
Human   753 QTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYARTSKQIMWFWQFVK 817

  Fly   971 SYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQN-LPKAHTCFNRID 1034
            ...:|.|.||||||||:.|:|:.||..|.||.   ||:.|.|.   .|..:| ||::||||||:|
Human   818 EIDNEKRMRLLQFVTGTCRLPVGGFADLMGSN---GPQKFCIE---KVGKENWLPRSHTCFNRLD 876

  Fly  1035 LPPYETYQLLCDKLTQAVEETCGFAVE 1061
            ||||::|:.|.:||..|:|||.||..|
Human   877 LPPYKSYEQLKEKLLFAIEETEGFGQE 903

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_523779.1	C2_Smurf-like	14..135	CDD:176028	25/120 (21%)
		PRP40	155..>283	CDD:227435	16/135 (12%)
		WW	169..198	CDD:459800	4/30 (13%)
		WW	514..546	CDD:197736	13/44 (30%)
		WW	562..594	CDD:197736	20/31 (65%)
		HECTc	702..1058	CDD:238033	174/357 (49%)
ITCH	NP_001244066.1	C2_E3_ubiquitin_ligase	17..140	CDD:175988	29/156 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	197..301		48/191 (25%)
		WW	328..357	CDD:459800	2/28 (7%)
		HUL4	<359..903	CDD:227354	233/595 (39%)
		Required for interaction with FYN. /evidence=ECO:0000269|PubMed:16387660	395..471		24/75 (32%)
		MAP kinase docking site. /evidence=ECO:0000250	574..583		5/8 (63%)