Protein Alignment Smurf and nedd4a

DIOPT Version :9

• Sequence 1: NP_001261061.1
• Gene: Smurf / 36999
• FlyBaseID: FBgn0029006
• Length: 1061
• Species: Drosophila melanogaster
• Sequence 2: NP_001029358.1
• Gene: nedd4a / 619412
• ZFINID: ZDB-GENE-051005-2
• Length: 910
• Species: Danio rerio
• Alignment Length: 1131
• Identity: 341/1131 - (30%)
• Similarity: 480/1131 - (42%)
• Gaps: 334/1131 - (29%)

  Fly    15 VRITILCARNLARKDLFRLPDPFAKVQ----VDGTGQVYSTEISKSSLDPKWNAHYDLFLGI--- 72
            :|:.|:....||:||:....||:.::.    |:|......|:..|.:||||||..:  |..:
Zfish    23 LRVKIIAGIGLAKKDILGASDPYTRISLYDPVNGEITSLQTKTIKKTLDPKWNEEF--FFRVHPR 85

  Fly    73 GDAITITVWNQRKIHKGSGFLGCVRIPAFNIQSLKGAGFQRLDLGK---LSP-DDDELVRGQIII 133
            ...:.:.|:::.::.: ..|||.|.:|...|.: :....:|....|   |.| .....|:|.:.:
Zfish    86 RHRLLLEVFDENRLTR-DDFLGQVDVPLHQIPT-EHPNNERPYTFKDFLLHPRSHKSRVKGHLRL 148

  Fly   134 SLL-------SKDGPS-SGNPLAIVGPSGDVRGPSEDDSSE-----DSLPEGWEERRTDNGRVYY 185
            .:.       .||.|: .|..|   .|..:...|.|..|..     .:||.|||||:.:.||.||
Zfish   149 KMTYLPKNRRCKDDPAEQGENL---DPGWEFLEPHELCSPHHPHQLPALPPGWEERQDNLGRTYY 210

  Fly   186 VNHATKSTQWDRPRQPGVVGSSHATSPQQRHNTHNGNSGDRQAPAGPTRSTTCTNLMNNGHRSRD 250
            |||.:::|||.||
Zfish   211 VNHESRTTQWHRP---------------------------------------------------- 223

  Fly   251 LSVTASDERRHSTEILSSVGKENTSPTTPVSATTTPGKKTSSSNSSSAGGRTLEQRPTNEPATPT 315
               |..|.:|.:                                         |||......:..
Zfish   224 ---TIQDSQRDN-----------------------------------------EQRQETHSESQR 244

  Fly   316 SSTTSASVRLHSNDNHVKTPKHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQ 380
            :..|...:..| .:||.:        ...|||
Zfish   245 AFLTRRQISEH-EENHER--------RESPES--------------------------------- 267

  Fly   381 SASNGW--TQEDAATTTSPSTTTSPPRHSQSPPTPNISPPASVTPSANGNVHSPNANSTPAGSGG 443
                 |  ...|.:|..|..:..|||            ||           |||...||..
Zfish   268 -----WEIITADESTLYSSLSERSPP------------PP-----------HSPMEISTFC---- 300

  Fly   444 GSRSYTAATPGQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSGGGGGGGSGQRYASAAIAAANQA 508
                                    |...|.:::...|...|              ....|||.:.
Zfish   301 ------------------------EEFNRLQATNDRRTSLT--------------PQVTAAAEEV 327

  Fly   509 ARP---------------FLDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRI------------ 546
            .||               ...||||:|.:...:|::||.:..:..:||..|.|
Zfish   328 DRPSRWRSILFTHSLLPTSAGLPPGWEEKQDSKGRIYFVNHNSRTTTWTRPLIQLIQPPSESPAS 392

  Fly   547 ------------------PRDFDTQHL-------TLDAIGPLPSGWEQRKTASGRVYFVDHNNRT 586
                              |.|...||.       |.:..|.:|:|||.|...|||.:|:|||.:|
Zfish   393 PMASGSTSLSQNAAGYALPPDVSPQHTHTHTHTHTPEHPGLMPTGWEVRSAPSGRPFFIDHNTKT 457

  Fly   587 TQFTDPRLSGSILQMIRRGTVPPTSAANAGTPAPPSATPATPSAAAAVPPQATPASNATPTTLTT 651
            |.:.||||...: ||.||.::.||...    |.||..             ::.....|..:||.|
Zfish   458 TTWDDPRLKVPV-QMRRRVSLDPTDLG----PLPPGW-------------ESECIVMAESSTLIT 504

  Fly   652 TTNP------PHRIVPDLPQGLLEGADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNE 710
            |..|      .:||.|.|...:        .|.||...|....|.:|:..........|.|.||.
Zfish   505 TQKPHNGKTHDYRIQPSLGPAV--------PYSRDYKQKYEYFRKKLKKPAEIPNRFELSVRRNA 561

  Fly   711 IFEESYRLIMKMRAKDMRK-RLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHY 774
            :.|:|||.|:.::..::.| ||.|:|:||:|||||||||||..|:|:||.||.||||:||..|:|
Zfish   562 VLEDSYRRILSVKRSELLKARLWVEFEGEKGLDYGGVAREWFFLISKEMFNPYYGLFEYSATDNY 626

  Fly   775 TLQINPDSGV-NPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDL 838
            ||||||:||: |.||||||.|:||..|:||:||..||..|..||||.:|.|||||.|:|.||.:.
Zfish   627 TLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDAFFIRPFYKMMLQKPITLQDMESVDSEY 691

  Fly   839 HRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGI 903
            ..||.|:||::.:. ::..|:::...||....||||||||.|.|.:.||:||:.|.:.:||:..|
Zfish   692 FNSLRWILENDPTD-LDLRFTIDEELFGQTHQHELKPGGADIVVNDTNKKEYIHLVMQWRFVDRI 755

  Fly   904 EQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNET-TQVLWFWQ 967
            ::|..|.::||.||||..|::.|||.||||::.|:..:||||||.||:.|:..|.. ..::|||:
Zfish   756 QRQMTAFKEGFYELIPQDLIKIFDENELELLMCGLGDVDVNDWRENTKYKNGYNPNHPAIIWFWK 820

  Fly   968 VVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNR 1032
            .|....:|.|.||||||||:||||:.||..|.||.   ||:||||....  ..:.||:|||||||
Zfish   821 TVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSN---GPQLFTIEQWG--TREKLPRAHTCFNR 880

  Fly  1033 IDLPPYETYQLLCDKLTQAVEETCGF 1058
            :||||.|:::.|.:||..|:|...||
Zfish   881 LDLPPXESFEELREKLHMAIENAQGF 906

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_001261061.1	C2_Smurf-like	14..135	CDD:176028	32/130 (25%)
		WW	169..198	CDD:278809	17/28 (61%)
		WW	514..546	CDD:197736	11/31 (35%)
		WW	562..594	CDD:197736	16/31 (52%)
		HECTc	702..1058	CDD:238033	182/358 (51%)
		HECTc	726..1058	CDD:214523	173/334 (52%)
nedd4a	NP_001029358.1	C2_NEDD4_NEDD4L	22..153	CDD:175999	32/133 (24%)
		WW	194..223	CDD:366073	17/28 (61%)
		WW	349..378	CDD:366073	10/28 (36%)
		WW	435..465	CDD:238122	16/29 (55%)
		HECTc	577..906	CDD:214523	173/334 (52%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100256
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.810