Protein Alignment Smurf and hecw1b

DIOPT Version :10

• Sequence 1: NP_523779.1
• Gene: Smurf / 36999
• FlyBaseID: FBgn0029006
• Length: 1061
• Species: Drosophila melanogaster
• Sequence 2: XP_021336549.1
• Gene: hecw1b / 563730
• ZFINID: ZDB-GENE-060503-2
• Length: 1555
• Species: Danio rerio
• Alignment Length: 1428
• Identity: 366/1428 - (25%)
• Similarity: 543/1428 - (38%)
• Gaps: 445/1428 - (31%)

  Fly     5 DYPRRNGTHKV---RITILCARNLARKDLFRLPDPFAKVQV-----------DGTGQVYSTEISK 55
            |.|:..|..::   .::...|..| :|.:|..|||:.|:.:           ...||...:.|..
Zfish   202 DVPQSQGRRRLISFSLSDFQATGL-KKGMFFNPDPYLKIAIHPGKHSIFPALPHHGQEKRSGIVC 265

  Fly    56 SSLDPKWNAHYDLFLGI-GDAITITVWNQ----RKIHKGSGFLGCVRIPAFNIQSLKGAGFQRLD 115
            ::::|.|......|:.: .|.:.|.|.::    |.|.|  .|||.:.:|...:......|.:.:.
Zfish   266 NTINPVWQRERFNFVSLPTDVLEIEVKDKFAKSRPIIK--RFLGKLSMPVQRLLEKHAIGDRVVS 328

  Fly   116 --LGKLSPDDDELVRGQI-----IISLLSKDGP----SSGNPLAIVGPSGDVRGPSEDDSSEDSL 169
              ||:..|  .|.|.||:     |.|.:..|..    |:..|:|.......   |..:|:.  ||
Zfish   329 YTLGRRLP--TEHVSGQLQFRFEITSSIHPDDEDMSLSTEQPVAAAEAQAQ---PQAEDTM--SL 386

  Fly   170 PEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSSHATSPQQRHNTHNGNSGDRQAPA---- 230
            ..|..|...|..        |.|.:.....:.|...::.|...::.........|:..|.|
Zfish   387 DLGAPELPLDGD--------TASVEMPMDVESGQSCAAEADVIEEECLEEEQQEGEAAAAAAAME 443

  Fly   231 -GPTRSTTCTNLMN--NGHRSRD------LSVTASDERRHSTEILSSVGKENTSPTTPVSAT--- 283
             ..|.:...|.:::  ||....:      .:..:||.|         .|:|:.:.:.|..|:
Zfish   444 QSETNAEETTQVLDPINGAEEEEAENNCAFAEESSDAR---------AGEEDEAISDPQPASEEP 499

  Fly   284 -------------TTPGKKTSSSNSSSAGGRTLEQRPTNEPATPTSSTTSASVRLHSNDNHVKTP 335
                         |||.:|....:...:...|:......||.||.|.    .:|:|...:.:...
Zfish   500 DADLEEDCSLRIRTTPRRKPRPCSLPVSELETVIASACGEPETPRSH----YIRIHHLLHSLPAT 560

  Fly   336 KHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDAATTTSPSTT 400
            :.||...|.||  |..|.|.:.                   ...:....|.|.||.|.|.:....
Zfish   561 QPQTGDSAGPE--PDRPMGVEE-------------------EDEEEEEEGVTLEDHALTPNGPRR 604

  Fly   401 TSPPRHSQ------------------------SPPTPNISPPA---SVTPSANGNVHSPNANSTP 438
            |.|...|.                        :..:|::||.|   .::|.|:|.:.:.:.:|
Zfish   605 TLPRSRSHERLSDLIQMLDGDGHPLGAEGQTGNQRSPDLSPVACCSHMSPRASGPIRAQSLDS-- 667

  Fly   439 AGSGGGSRSYTAATPGQRSQRRSSRQQG--------EESSTRRRSSRG-------------TRNG 482
                 ..||.:.....|..:....:..|        |:.:..|....|             :.:|
Zfish   668 -----ARRSESTVFSSQDDEDEMEQPNGILGSETETEQGAQCREPGDGSCQWEEAPDSHVQSPHG 727

  Fly   483 GTSGGGGGGGSGQRYASAAI--AAANQ--AAR------PFLD--LPPGYEMRTTQQGQVYFYHIP 535
            ....|...|.||:|......  .|.:|  |.|      |.:|  |||.:|.|....|:|::....
Zfish   728 IVGNGEVAGPSGRRECPLPCNHPAVSQLPALRPDPHHYPTIDEPLPPNWEARIDSHGRVFYVDHI 792

  Fly   536 TGVSTWHDP-------------------RIPRDFDT----------------------------- 552
            ...:||..|                   ::.|.:.|
Zfish   793 NRTTTWQRPTSAATPDGLRRSGSVQQMEQLNRRYQTIQRTMATERREEESGSPRNDRTANTETDS 857

  Fly   553 ----------------------------------------------------------QHLTL-- 557
                                                                      :|:.|
Zfish   858 PLQTNELRRDTSCSPGHCQKITLLLQSPAVKFITHPEFFTVLHANYGAYRMFTNSSCLKHMVLKI 922

  Fly   558 ------------------------DAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL---S 595
                                    ||...||.|||.:....|:.:|||||:|.|.|.|||:   :
Zfish   923 RRDARNFERYQHNRDLVTFLNRFADAQLELPRGWEIKTDPQGKSFFVDHNSRATTFIDPRIPLQN 987

  Fly   596 GSI---------LQMIRRGTVPPTSAANAGTPAPPSATP-----ATP--SAAAAV--PPQATPAS 642
            |.:         ||.:|        :.:||..:..|.|.     |.|  |..||:  ..|..|..
Zfish   988 GRLPNHLTHRQHLQRLR--------SYSAGEASEVSRTRGVSLLARPGNSLVAAIRNQSQQEPVP 1044

  Fly   643 NATPTTLTTTTNPP-----------------------HRI------------------------- 659
            .|....:......|                       |.|
Zfish  1045 LAYNDKIVAFLRQPNIFEVLQERQPSFARNHSLKDKVHHIRTEGTQRLEKLSCDADLVMLLSLFE 1109

  Fly   660 ------VP-----------------DLPQ---GLLEGADLLP-------------------KYRR 679
                  ||                 ..||   |.||...|:|                   .|||
Zfish  1110 EDIMSYVPLASFHPGFNFSPRCSPGSSPQNSPGFLERTPLVPACCLSGESSPGTQRARAPAPYRR 1174

  Fly   680 DLVGKLRALRTELQT----MQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDM-RKRLMVKFKGEE 739
            |...|||....:|:.    ..|  |..:|.|.|:.:.|.::..:|....|:: |.:|.:.|.|||
Zfish  1175 DFEAKLRNFYRKLEAKGYGQGP--GKIKLIVRRDHLLEGTFNQVMAYSRKELQRNKLYITFVGEE 1237

  Fly   740 GLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVF 804
            ||||.|.:||:..|||:|:.||.||||:||.:|.||:||:|.|....:||.:|.|.||.||:|:.
Zfish  1238 GLDYSGPSREFFFLLSQELFNPYYGLFEYSANDTYTVQISPMSAFVENHLEWFRFSGRILGLALI 1302

  Fly   805 HGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALV 869
            |.:.||..||.||||.||..|..|.|:|.:|.:.|:||.||.|::|:.:::.||:|....||.:.
Zfish  1303 HQYLLDAFFTRPFYKALLRLPTDLSDLEYLDEEFHQSLQWMKENDITDVLDLTFTVNEEVFGQVT 1367

  Fly   870 VHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELV 934
            ..|||.||.::.|||:||:||::..|.:|..||:.||..||.:||.|::.|.|:..||.||||||
Zfish  1368 ERELKSGGTNVQVTEKNKKEYIERMVKWRVERGVVQQTQALVRGFYEVVDSRLVSVFDARELELV 1432

  Fly   935 IGGISSIDVNDWRNNTRLKHCTNETTQVL-WFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRAL 998
            |.|.:.||:|||||||..:...::...|: |||..||.:::|.|.||||||||:|.||.:||.||
Zfish  1433 IAGTAEIDLNDWRNNTEYRGGYHDGHIVIRWFWGAVERFNNEQRLRLLQFVTGTSSVPYEGFTAL 1497

  Fly   999 QGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061
            :||.|.   |.|.|.....:  .:||:|||||||:|||||.:|.:|.:||..|||||..|.:|
Zfish  1498 RGSNGL---RRFCIEKWGKI--TSLPRAHTCFNRLDLPPYPSYTMLYEKLLIAVEETSTFGLE 1555

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_523779.1	C2_Smurf-like	14..135	CDD:176028	33/146 (23%)
		PRP40	155..>283	CDD:227435	24/140 (17%)
		WW	169..198	CDD:459800	6/28 (21%)
		WW	514..546	CDD:197736	11/52 (21%)
		WW	562..594	CDD:197736	16/31 (52%)
		HECTc	702..1058	CDD:238033	171/357 (48%)
hecw1b	XP_021336549.1	HECW_N	73..190	CDD:465177
		C2_NEDL1-like	213..349	CDD:176073	32/140 (23%)
		WW	772..801	CDD:459800	9/28 (32%)
		HECW1_helix	880..946	CDD:465766	2/65 (3%)
		WW	952..983	CDD:197736	16/30 (53%)
		HECTc	1223..1552	CDD:214523	165/333 (50%)