Protein Alignment Smurf and nedd4l

DIOPT Version :10

• Sequence 1: NP_523779.1
• Gene: Smurf / 36999
• FlyBaseID: FBgn0029006
• Length: 1061
• Species: Drosophila melanogaster
• Sequence 2: XP_021324693.1
• Gene: nedd4l / 559634
• ZFINID: ZDB-GENE-051118-2
• Length: 1274
• Species: Danio rerio
• Alignment Length: 1160
• Identity: 364/1160 - (31%)
• Similarity: 522/1160 - (45%)
• Gaps: 261/1160 - (22%)

  Fly     6 YPRRNGTHKVRITILCARNL----ARKDLFRLPD---PFAKVQVD-------GTGQVYSTEISKS 56
            |||      :|:....::.|    |...|..:|:   |.::::.:       ..|..:.....::
Zfish   265 YPR------IRLERSTSQPLQPRGAPSRLGSMPNLHAPISEMETESDFIENGAQGCTFKIRKEQN 323

  Fly    57 SLDPKWNAHY----------DLFLGIGDAIT-------ITVWNQRKIHKGSG-----FLGCVRIP 99
            |..||:..::          :..:.||.|.|       |.:...:..|..:|     |||.|.:|
Zfish   324 SQQPKFKIYFSQGGEHSPILERNISIGKAPTCSNPQAHIGLDRPKNGHFNNGQTRDDFLGQVDVP 388

  Fly   100 AFNIQSLKGAGFQRLDLGK---LSP-DDDELVRGQIIISL--LSKDGPSSGNPLAIVGPSGDVRG 158
            ..::.: :....:|....|   |.| .....|:|.:.:.:  |.|:|.....       ||::|.
Zfish   389 LTHLPT-EDPAMERPYTFKDFLLRPRSHKSRVKGYLRLKMAYLPKNGGHEEE-------SGEMRE 445

  Fly   159 PSEDDSSEDS--------------LPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSSHA 209
            .:|.....||              ||.||||:..:.||.|||||..::|||.||....||.
Zfish   446 EAEGWEVTDSTDPGSQRPQQLLPPLPPGWEEKVDNLGRTYYVNHNNRTTQWKRPSSVDVVS---- 506

  Fly   210 TSPQQRHNTHNGNSGDRQAPAGPTRSTTCTNLMNNGHRSRDLSVTASDERRHSTEILSSVG---K 271
                   .|.|.|.              ...:....||       ....|||.:|.|.:..   :
Zfish   507 -------ETENDNQ--------------LRQINQEAHR-------VFRSRRHISEDLENEQLDIR 543

  Fly   272 ENTSPTTPVSATTTPGKKTSSSNSSSAGGRTLEQRPTNEPATPTSST----TSASVRLHSNDNHV 332
            :......|:| ...|...|...|.|.:|..:|        |.|.|||    ...|:||
Zfish   544 DIDDSWEPIS-EEDPTLLTDGLNHSLSGPSSL--------AMPLSSTPDFSDEISLRL------- 592

  Fly   333 KTPKHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDAATTTSP 397
             :....|||..|..|:..     |::::...::.|.:...|.||..|....:..|:...|.|.:.
Zfish   593 -SLAPDTNGEIPGPSSAV-----QSHLSSRLRSSSMTDGVSDQAQPPTPTQSSQTRRTRAQTVTG 651

  Fly   398 STTTSPPRHSQSPPTPNISP-------PASVTPSANGN------------VHSPNANSTPAGSGG 443
            ...|..|..|.:..||.:.|       |...|...|.|            .|:.:..||...:.|
Zfish   652 VEDTMSPSASYALTTPGLPPGWEERKDPKGRTYYVNHNNRSTTWTRPILQQHTEDGASTSVAAVG 716

  Fly   444 GSRSYTAATPGQRSQRRSSRQQGEESSTRRRSSRG---TRNGGTSGGG------------GGGGS 493
            |:   |||||...:...||....|....|.||...   |.:....|..            ....|
Zfish   717 GA---TAATPPASTPSTSSGHLSEPQVRRPRSLSSPTVTLSSPLEGANNVPIRRAVKDTFSNPQS 778

  Fly   494 GQRYASAAIAAANQAARPFLDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRI--PRDFDTQHLT 556
            .|....::....::|.:.|  ||||:|||....|:.:|....:..:||.|||:  |....|: .:
Zfish   779 PQPSPYSSPKTQHKANQSF--LPPGWEMRIAPNGRPFFIDHNSRTTTWEDPRLKYPVHMRTK-AS 840

  Fly   557 LDA--IGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRLSGSILQMIRRGTVPPTSAANAGTPA 619
            ||.  :||||.|||:|..|.||.:::||||:.||:.||||.                       :
Zfish   841 LDPGDLGPLPPGWEERVHADGRTFYIDHNNKKTQWEDPRLQ-----------------------S 882

  Fly   620 PPSATPATPSAAAAVPPQATPASNATPTTLTTTTNPPHRIVPDLPQGLLEGADLLPKYRRDLVGK 684
            |....||.|                                                |.|:...|
Zfish   883 PAITGPAVP------------------------------------------------YSREFKQK 899

  Fly   685 LRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKD-MRKRLMVKFKGEEGLDYGGVAR 748
            ....|.:|:..........:::.||.|||||||.||.::..| ::.||.::|:.|:|||||||||
Zfish   900 YDYFRKKLKKPADIPNRFEMKLHRNNIFEESYRRIMSLKRPDSLKARLWIEFESEKGLDYGGVAR 964

  Fly   749 EWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGV-NPDHLSYFHFVGRTLGIAVFHGHCLDGG 812
            ||..|||:||.||.||||:||..|:|||||||:||: |.||||||.|:||..|:||:||..|||.
Zfish   965 EWFFLLSKEMFNPYYGLFEYSATDNYTLQINPNSGLCNEDHLSYFKFIGRVAGMAVYHGKLLDGF 1029

  Fly   813 FTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGG 877
            |..||||.:|.|.|||.|:|.||.:.:.||.|:||::.:. ::..|.::.::||.....:|||.|
Zfish  1030 FIRPFYKMMLGKQITLNDMESVDSEYYNSLKWILENDPTE-LDLRFCIDEDNFGQTYQVDLKPSG 1093

  Fly   878 ASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSID 942
            :.:.||.:||:||:.|.:.:||:..:::|..|..:||.||||..|::.|||.||||::.|:..:|
Zfish  1094 SDMVVTNDNKKEYIDLVIQWRFVNRVQKQMNAFLEGFTELIPIDLIKIFDENELELLMCGLGDVD 1158

  Fly   943 VNDWRNNTRLK--HCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAV 1005
            |||||.:|..|  :|.|... :.|||:.|....:|.|.||||||||:||||:.||..|.||.
Zfish  1159 VNDWRQHTVYKNGYCPNHPV-IQWFWKAVLLMDAEKRIRLLQFVTGTSRVPMNGFAELYGSN--- 1219

  Fly  1006 GPRLFTIHL--TADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGF 1058
            ||:||||..  |.|    .||:|||||||:|||.|||::.|.:||..|||...||
Zfish  1220 GPQLFTIEQWGTPD----KLPRAHTCFNRLDLPMYETFEDLREKLLMAVENAQGF 1270

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_523779.1	C2_Smurf-like	14..135	CDD:176028	28/160 (18%)
		PRP40	155..>283	CDD:227435	36/144 (25%)
		WW	169..198	CDD:459800	16/28 (57%)
		WW	514..546	CDD:197736	13/31 (42%)
		WW	562..594	CDD:197736	18/31 (58%)
		HECTc	702..1058	CDD:238033	182/361 (50%)
nedd4l	XP_021324693.1	C2	<374..430	CDD:472691	12/56 (21%)
		WW	473..499	CDD:459800	14/25 (56%)
		WW	669..698	CDD:459800	5/28 (18%)
		WW	798..829	CDD:197736	13/30 (43%)
		WW	848..880	CDD:197736	18/31 (58%)
		HECTc	941..1270	CDD:214523	171/337 (51%)