Protein Alignment Smurf and Nedd4

DIOPT Version :9

• Sequence 1: NP_001261061.1
• Gene: Smurf / 36999
• FlyBaseID: FBgn0029006
• Length: 1061
• Species: Drosophila melanogaster
• Sequence 2: NP_648993.1
• Gene: Nedd4 / 39958
• FlyBaseID: FBgn0259174
• Length: 1007
• Species: Drosophila melanogaster
• Alignment Length: 1131
• Identity: 363/1131 - (32%)
• Similarity: 516/1131 - (45%)
• Gaps: 260/1131 - (22%)

  Fly     7 PRR---------NGTHKVRITILCARNLARKDLFRLPDPFAKVQ---VDGTGQVYS--TEISKSS 57
            |||         :..| :||.:|..::||:||:|...||:.::.   ::|...:.|  |:..|.:
  Fly    56 PRRSLAAVNDSGDSCH-LRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKT 119

  Fly    58 LDPKWNAHYDLFLGIGD-AITITVWNQRKIHKGSGFLGCVRIPAFNI---QSLKGAGFQRLDL-G 117
            |:|.||..:...:...: .:...|:::.::.: ..|||.|.:...|:   |..:..|.|...| .
  Fly   120 LNPTWNEEFIFRVKPSEHKLVFQVFDENRLTR-DDFLGMVELTLVNLPTEQEGRTIGEQSYTLRP 183

  Fly   118 KLSPDDDELVRGQI------IISLLSKDGPSSGNPLAIVGPSGDVRGPSEDDSS----------E 166
            :.|......::|.:      |.....:..|||||.   .|....|...:..::|          .
  Fly   184 RRSVGAKSRIKGTLRIYHAFIRETREQSEPSSGNS---DGEWEHVEATNAGETSAQPHPFPTGGH 245

  Fly   167 DSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSSHATSPQQRHNTHNGNSGDRQAPAG 231
            |:||.|||||:..|||.|||||..::||||||.   |:            |:|:..|.|.|
  Fly   246 DALPAGWEERQDANGRTYYVNHTARTTQWDRPT---VL------------NSHSSQSTDDQ---- 291

  Fly   232 PTRSTTCTNLMNNGHRSRDLSVTASDERRHSTEILSSVGKENTSPT----TPVSATTTPGKKTSS 292
                     |.::..|...:||..::..|.:..|..:..::|.:..    ||.:|.|:.....:.
  Fly   292 ---------LASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAPPNTP 347

  Fly   293 SNSSSAGGRTLEQRPTNEPATPTSSTTSASVRLHSNDNHVKTPKHQTNGHAPPESTPTSPTGQQN 357
            :|::....:...|....|...||...||...      |.::.|.    .|..||.:.||
  Fly   348 TNNNGILAQIAMQYRAEEDQDPTVDHTSFVY------NSLRHPV----AHRQPEISATS------ 396

  Fly   358 YVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDAATTTSPSTTTSPPRHSQSPPTPNISPPASVT 422
                                         .|.|          ..|.|  ::|..|:|:.....|
  Fly   397 -----------------------------LQND----------LRPVR--EAPGVPDIAITNPFT 420

  Fly   423 PSANGNVHSPNANSTPAGSGGGS----RSYTAATPGQRSQRRSSRQQGE-----ESSTRRRSSRG 478
            ..|.||:         ||..|..    |........|..||:..:||..     :...:....||
  Fly   421 RRAAGNM---------AGGAGWQQERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRG 476

  Fly   479 TR--------NGGTSGGGGGGGSGQRYASAAIAAAN----QAARPFLD---------LPPGYEMR 522
            .|        |..|........|.   .||.....|    .||.|.::         |||.:.|:
  Fly   477 QRHQQQHRPSNEDTDHTDSHNPSD---ISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQ 538

  Fly   523 TTQQGQVYFYHIPTGVSTWHDPR----IPRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHN 583
            ....|:.:|....:..:||.|||    .|....|:.:. |.:||||.|||:|....|||:::|||
  Fly   539 VAPNGRTFFIDHASRRTTWIDPRNGRASPMPNQTRRVE-DDLGPLPEGWEERVHTDGRVFYIDHN 602

  Fly   584 NRTTQFTDPRLSGSILQMIRRGTVPPTSAANAGTPAPPSATPATPSAAAAVPPQATPASNATPTT 648
            .||||:.|||||                               .|:.|.    ||.|
  Fly   603 TRTTQWEDPRLS-------------------------------NPNIAG----QAVP-------- 624

  Fly   649 LTTTTNPPHRIVPDLPQGLLEGADLLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFE 713
                                        |.||...|....::.::..........:.:.|..|.|
  Fly   625 ----------------------------YSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILE 661

  Fly   714 ESYRLIMKMRAKDMRK-RLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQ 777
            :|||:|..:...|:.| :|.|:|:||.||||||:||||.:|||:||.||.||||:||..|:||||
  Fly   662 DSYRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQ 726

  Fly   778 INPDSGV-NPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRS 841
            ||..||: |.:|||||.|:||..|:||:||..||..|..||||.:|.|||.|.|:|.||.:.:.|
  Fly   727 INNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNS 791

  Fly   842 LTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQ 906
            |.|:.| |...|:|.||.::.:.||....||||||||:|.||.|||.||:||.:.:||:..:::|
  Fly   792 LMWIKE-NDPRILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQ 855

  Fly   907 FLALQKGFCELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLK---HCTNETTQVLWFWQV 968
            ..:...||..:||.:|::.|||.||||::.||.:|||.|||.||..|   |..:...|  |||:.
  Fly   856 MSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQ--WFWRA 918

  Fly   969 VESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRI 1033
            |.|:|:|||:||||||||:||||:.||:.|.||.   ||::|||....  ...|.|:|||||||:
  Fly   919 VLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSN---GPQMFTIEKWG--TPNNFPRAHTCFNRL 978

  Fly  1034 DLPPYETYQLLCDKLTQAVEETCGFA 1059
            ||||||.|..|.|||.:|:|.:.|||
  Fly   979 DLPPYEGYLQLKDKLIKAIEGSQGFA 1004

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_001261061.1	C2_Smurf-like	14..135	CDD:176028	31/136 (23%)
		WW	169..198	CDD:278809	19/28 (68%)
		WW	514..546	CDD:197736	11/44 (25%)
		WW	562..594	CDD:197736	19/31 (61%)
		HECTc	702..1058	CDD:238033	186/360 (52%)
		HECTc	726..1058	CDD:214523	179/336 (53%)
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	31/134 (23%)
		WW	248..277	CDD:278809	19/28 (68%)
		WW	531..560	CDD:278809	8/28 (29%)
		WW	581..613	CDD:197736	19/31 (61%)
		HECTc	650..1003	CDD:238033	186/360 (52%)
		HECTc	674..1003	CDD:214523	179/336 (53%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C45446959
Domainoid	1	1.000	49	1.000	Domainoid score	I7919
eggNOG	1	0.900	-	-		E1_COG5021
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D13657at50557
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100256
Panther	1	1.100	-	-	P	PTHR11254
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2273
SonicParanoid	0	0.000	Not matched by this tool.
	9	8.780