DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Smurf and Hecw2

DIOPT Version :9

Sequence 1:	NP_001261061.1	Gene:	Smurf / 36999	FlyBaseID:	FBgn0029006	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	NP_001001883.1	Gene:	Hecw2 / 329152	MGIID:	2685817	Length:	1578	Species:	Mus musculus

Alignment Length:	1433	Identity:	383/1433 - (26%)
Similarity:	558/1433 - (38%)	Gaps:	450/1433 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 RKDLFRLPDPFAKVQVD-----------GTGQVYSTEISKSSLDPKWNAH-YDLFLGIGDAITIT 79
            :|.:|..|||:.|:.:.           ..||...:.|..::.:|.|:.. |..|..:.|.:.|.
Mouse   198 KKGMFFNPDPYLKMSIQPGKKSSFPTCAHHGQERRSTIISNTTNPIWHREKYSFFALLTDVLEIE 262

  Fly    80 VWNQ----RKIHKGSGFLGCVRIPAFNIQSL--KGAGFQRL--DLGKLSPDDDELVRGQI----- 131
            :.::    |.|.|  .|||.:.||   :|.|  :.||.|.|  :||:..|.|.  |.|.:
Mouse   263 IKDKFAKSRPIIK--RFLGKLTIP---VQRLLERQAGDQMLSYNLGRRLPADH--VSGYLQFKVE 320

  Fly   132 IISLLSKD-GPSS-GNPLAIVGPSGDVRGPSEDDS----------------SEDSLPEGWEER-- 176
            :.|...:| .|.: |..|.:...:||:..||:::.                ||||:.:|..:.
Mouse   321 VTSSAHEDASPEAVGTILGVHTVNGDLGSPSDEEDMPGSHHDSTICANGPVSEDSVADGTPKHSF 385

  Fly   177 RTDN--------------------GRVYYVNHATKSTQWDRPRQPGVVGSS-------------- 207
            ||.:                    ||...:|....:.:.:.|.:||...||
Mouse   386 RTSSTLEIDTEDLISTSSRNSPPRGRQDSLNDYLDAIEHNGPARPGAASSSERSMGASPKLRSSF 450

  Fly   208 ----------HATSPQQRHNTHNG----------------------NSGDRQAPAGPTRSTTCTN 240
                      |..|.::.|.....                      :.|...:...|.......|
Mouse   451 PTDTRLNAMLHIDSDEEDHEFQQDLGYPSSLEEEGGLIMCSRASRIDDGSLTSQTKPEDDNPVEN 515

  Fly   241 LMNNGHRSRDL----------------SVTASDER-----------RHSTEILSSVGKENTSPTT 278
            ...:.|.:..|                |.||.||.           :.|||:.||  :|...||:
Mouse   516 EDASIHETASLEERLENLPEVADGSLPSSTAPDENEANLEPQPSADQGSTELCSS--QEVDQPTS 578

  Fly   279 PVSA---TTTPGKKTSSSNSSS--AGGRTLEQRPTNEPATP--TSSTTSASVRLHSNDNHVKTPK 336
            ...|   .|:.|.:.::|.:.|  .|....:.....||:.|  |.|.:.||.|          |:
Mouse   579 GADAGASDTSGGSRRAASETESLDQGSEPSQVSSETEPSDPARTESVSEASTR----------PE 633

  Fly   337 HQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQS-ASNGWTQEDAATTTSPSTT 400
            .:::......|...|.|.|.:.|       .|..:....|..|:: |.:...:||.|....| |:
Mouse   634 GESDPEGADSSCNESVTTQLSSV-------ETRCSSLESARFPETPAFSSQEEEDGACAAEP-TS 690

  Fly   401 TSPPRHSQ-SPPTPNISPPASVTPS--ANGNVHSPNANSTPAGSG------GGSRSYTAATPGQR 456
            :.|...|| |..||: |.||...||  ..|:.....|.|.....|      |......||.|...
Mouse   691 SGPAEGSQESVCTPS-SLPAVQVPSREEEGSAAEAAALSEQGELGEVWQRRGSLEGAAAAAPAAA 754

  Fly   457 SQRRSSRQQGEESSTRRRSSRGTRNGGTS---GGGGGGGSGQRYASAAIAAANQAARPF--LD-- 514
            :.....::.|:..     .::|...|.|:   |..||..:.......::.:..|....:  :|
Mouse   755 ATDSQPQEDGDAG-----DAQGACEGATAQEEGATGGSQTNGHQPLRSLPSVRQDVSRYQRVDEA 814

  Fly   515 LPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIP-------------------------------- 547
            |||.:|.|....|::::.......:||..|..|
Mouse   815 LPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQRSNSIQQMEQLNRRYQSIRRTMTNE 879

  Fly   548 ----------------------RDF---------------------------------------- 550
                                  .||
Mouse   880 RPEENTSAIDGAGEEADFHQASADFRRENVLPHSTSRSRLTLLLQSPPVKFLISPEFFTVLHSNP 944

  Fly   551 ---------------------DTQHL-----TLDAIG----------PLPSGWEQRKTASGRVYF 579
                                 ||.|.     ..|.:|          .||.|||.:....|:.:|
Mouse   945 SAYRMFTNNTCLKHMITKVRRDTHHFERYQHNRDLVGFLNMFANKQLELPRGWEMKHDHQGKAFF 1009

  Fly   580 VDHNNRTTQFTDPRLSGSILQMIRRGTVPPTSA----------------------ANAGTPAPPS 622
            ||||:|||.|.||||.   ||..|     ||||                      .:||.|..|.
Mouse  1010 VDHNSRTTTFIDPRLP---LQSSR-----PTSALVHRQHLTRQRSHSAGEVGEDSRHAGPPVLPR 1066

  Fly   623 A-----TPATPSAAAAVP-----------------------------------------PQATP- 640
            .     |.:.|.....||                                         .:.||
Mouse  1067 PSSTFNTVSRPQYQDMVPVAYNDKIVAFLRQPNILEILQERQPDLARNHSLREKIQFIRTEGTPG 1131

  Fly   641 ----ASNATPTTLTTTTN-------PPHRIV---------------PDLPQGLLEGADLLP-KYR 678
                :|:|....|.:...       |||.::               |....|........| .|:
Mouse  1132 LVRLSSDADLVMLLSLFEEEIMSYVPPHALLHPSYCQSPRGSPVSSPQNSPGTQRANARAPAPYK 1196

  Fly   679 RDLVGKLRALRTELQT----MQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDM-RKRLMVKFKGE 738
            ||...|||....:|:|    ..|  |..:|.:.|:.:.|:::..||....||: |.:|.|.|.||
Mouse  1197 RDFEAKLRNFYRKLETKGYGQGP--GKLKLIIRRDHLLEDAFNQIMGYSRKDLQRNKLYVTFVGE 1259

  Fly   739 EGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAV 803
            |||||.|.:||:..|:|||:.||.||||:||.:|.||:||:|.|....:|..:|.|.||.||:|:
Mouse  1260 EGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLAL 1324

  Fly   804 FHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGAL 868
            .|.:.||..||.||||.||.....|.|:|.:|.:.|:||.||.:::|..|::.||:|....||.:
Mouse  1325 IHQYLLDAFFTRPFYKALLRILCDLSDLEYLDEEFHQSLQWMKDNDIHDILDLTFTVNEEVFGQI 1389

  Fly   869 VVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELEL 933
            ...|||||||:|||||:||:||::..|.:|..||:.||..:|.:||.|::.:.|:..||.|||||
Mouse  1390 TERELKPGGANIPVTEKNKKEYIERMVKWRIERGVVQQTESLVRGFYEVVDARLVSVFDARELEL 1454

  Fly   934 VIGGISSIDVNDWRNNTRLKHCTNETTQVL-WFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRA 997
            ||.|.:.||:|||||||..:...::...|: |||..||.:::|.|.||||||||:|.:|.:||.:
Mouse  1455 VIAGTAEIDLNDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFAS 1519

  Fly   998 LQGSTGAVGPRLFTIH----LTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGF 1058
            |:||.   |||.|.:.    :||      ||:|||||||:|||||.::.:|.:||..|||||..|
Mouse  1520 LRGSN---GPRRFCVEKWGKITA------LPRAHTCFNRLDLPPYPSFSMLYEKLLTAVEETSTF 1575

  Fly  1059 AVE 1061
            .:|
Mouse  1576 GLE 1578

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_001261061.1	C2_Smurf-like	14..135	CDD:176028	35/132 (27%)
		WW	169..198	CDD:278809	6/50 (12%)
		WW	514..546	CDD:197736	10/33 (30%)
		WW	562..594	CDD:197736	17/31 (55%)
		HECTc	702..1058	CDD:238033	172/361 (48%)
		HECTc	726..1058	CDD:214523	166/337 (49%)
Hecw2	NP_001001883.1	HECW_N	45..164	CDD:374632
		C2_NEDL1-like	185..320	CDD:176073	35/128 (27%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	341..453		19/111 (17%)
		DMP1	<344..700	CDD:369294	72/375 (19%)
		NESP55	<463..639	CDD:115071	35/187 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	496..802		73/331 (22%)
		Interaction with TP73. /evidence=ECO:0000250	737..1074		60/349 (17%)
		WW	815..844	CDD:366073	8/28 (29%)
		HECW1_helix	921..987	CDD:375862	5/65 (8%)
		WW	993..1022	CDD:366073	15/28 (54%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1030..1075		10/49 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1167..1193		2/25 (8%)
		HECTc	1222..1576	CDD:238033	172/362 (48%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.810