DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Smurf and pub1

DIOPT Version :9

Sequence 1:	NP_001261061.1	Gene:	Smurf / 36999	FlyBaseID:	FBgn0029006	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	NP_594396.1	Gene:	pub1 / 2541818	PomBaseID:	SPAC11G7.02	Length:	767	Species:	Schizosaccharomyces pombe

Alignment Length:	1104	Identity:	345/1104 - (31%)
Similarity:	475/1104 - (43%)	Gaps:	397/1104 - (35%)

- Green bases have known domain annotations that are detailed below.

  Fly    12 THKVRITILCARNLARKDLFRLPDPFAKVQVDGTGQVYSTEISKSSLDPKWNAHYDLFLGIGDAI 76
            :.::|:||:.|..|.::|:||.|||||.:.|||. |.::|...|.:|:|.||..:::.:.....|
pombe     7 SRRIRVTIVAADGLYKRDVFRFPDPFAVLTVDGE-QTHTTTAIKKTLNPYWNETFEVNVTDNSTI 70

  Fly    77 TITVWNQRKI-HKGSGFLGCVRIPAFNIQSLKGAGFQRLDLGKLSPDDDELVRGQIIISL----- 135
            .|.|::|:|. .||.||||.:.:...::..|...|.:.|.......:::.:|.|:|||:|
pombe    71 AIQVFDQKKFKKKGQGFLGVINLRVGDVLDLAIGGDEMLTRDLKKSNENTVVHGKIIINLSTTAQ 135

  Fly   136 -------------------LSKDGPSSGNPLAIVGPSGDVRG-PSEDD---------------SS 165
                               ::.|..||.:......|:....| |:.|:               |.
pombe   136 STLQVPSSAASGARTQRTSITNDPQSSQSSSVSRNPASSRAGSPTRDNAPAASPASSEPRTFSSF 200

  Fly   166 EDS---LPEGWEERRTDN-GRVYYVNHATKSTQWDRPRQPGVVGSSHATSPQQRHNTHNGNSGDR 226
            ||.   ||.|| |||||| ||.|||:|.|:||.|.||....|.|::.|
pombe   201 EDQYGRLPPGW-ERRTDNLGRTYYVDHNTRSTTWIRPNLSSVAGAAAA----------------- 247

  Fly   227 QAPAGPTRSTTCTNLMNNGHRSRDLSVTASDERRHSTEILSSVGKENTSPTTPVSATTTPGKKTS 291
                                                 |:.||..          ||..|.|.:.|
pombe   248 -------------------------------------ELHSSAS----------SANVTEGVQPS 265

  Fly   292 SSNSSSAGGRTLEQRPTNEPATPTSSTTSASVRLHSNDNHVKTPKHQTNGHAPPESTPTSPTGQQ 356
            |||
pombe   266 SSN-------------------------------------------------------------- 268

  Fly   357 NYVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDAATTTSPSTTTSPPRHSQSPPTPNISPPASV 421
                                              ||..|..|..||
pombe   269 ----------------------------------AARRTEASVLTS------------------- 280

  Fly   422 TPSANGNVHSPNANSTPAGSGGGSRSYTAATPGQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSG 486
                         |:|.||||
pombe   281 -------------NATTAGSG-------------------------------------------- 288

  Fly   487 GGGGGGSGQRYASAAIAAANQAARPFLDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRI----- 546
                                       :||||:|.|.|.:|:.||....|..:||.|||.
pombe   289 ---------------------------ELPPGWEQRYTPEGRPYFVDHNTRTTTWVDPRRQQYIR 326

  Fly   547 ----PRDFDTQHLTLDAIGPLPSGWEQRKTASGRVYFVDHNNRTTQFTDPRLSGSILQMIRRGTV 607
                |.:...|...:..:||||||||.|.|.:.||||||||.:||.:.||||..|:.|.:
pombe   327 SYGGPNNATIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDDPRLPSSLDQNV----- 386

  Fly   608 PPTSAANAGTPAPPSATPATPSAAAAVPPQATPASNATPTTLTTTTNPPHRIVPDLPQGLLEGAD 672

pombe   387 ----------------------------------------------------------------- 386

  Fly   673 LLPKYRRDLVGKLRALRTELQTMQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKG 737
              |:|:||...||....:: ..:.|..|.|.::|.||.|||:||..||:..|.|::||||:||.|
pombe   387 --PQYKRDFRRKLIYFLSQ-PALHPLPGQCHIKVRRNHIFEDSYAEIMRQSATDLKKRLMIKFDG 448

  Fly   738 EEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIA 802
            |:||||||::||:..|||.||.||.|.||:||..|:|||||||.||:||:||:||.|:||.:|:|
pombe   449 EDGLDYGGLSREYFFLLSHEMFNPFYCLFEYSSVDNYTLQINPHSGINPEHLNYFKFIGRVIGLA 513

  Fly   803 VFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGA 867
            :||...:|..|...|||.:|.|.:||.|:|.:|.:.:|||.|:|:::|:|:::.|||||:|.||.
pombe   514 IFHRRFVDAFFVVSFYKMILQKKVTLQDMESMDAEYYRSLVWILDNDITGVLDLTFSVEDNCFGE 578

  Fly   868 LVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELE 932
            :|..:|||.|.:|.||||||||||.|...:|..:.||:||.|..:||.||||..|:..|||||||
pombe   579 VVTIDLKPNGRNIEVTEENKREYVDLVTVWRIQKRIEEQFNAFHEGFSELIPQELINVFDERELE 643

  Fly   933 LVIGGISSIDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRA 997
            |:|||||.||:.||:.:|..:..:.....:.|||::::.:|:|.::|||||.||:||:|:.||:.
pombe   644 LLIGGISEIDMEDWKKHTDYRSYSENDQIIKWFWELMDEWSNEKKSRLLQFTTGTSRIPVNGFKD 708

  Fly   998 LQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061
            ||||.   |||.|||....:  ...||||||||||:|||||.:.:.|..||:.|||||.||..|
pombe   709 LQGSD---GPRKFTIEKAGE--PNKLPKAHTCFNRLDLPPYTSKKDLDHKLSIAVEETIGFGQE 767

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_001261061.1	C2_Smurf-like	14..135	CDD:176028	42/121 (35%)
		WW	169..198	CDD:278809	20/29 (69%)
		WW	514..546	CDD:197736	15/31 (48%)
		WW	562..594	CDD:197736	21/31 (68%)
		HECTc	702..1058	CDD:238033	189/355 (53%)
		HECTc	726..1058	CDD:214523	177/331 (53%)
pub1	NP_594396.1	HUL4	1..767	CDD:227354	344/1102 (31%)
		C2_Smurf-like	9..131	CDD:176028	42/122 (34%)
		WW	207..236	CDD:278809	20/29 (69%)
		WW	290..319	CDD:278809	13/28 (46%)
		WW	346..378	CDD:197736	21/31 (68%)
		HECTc	413..765	CDD:238033	190/356 (53%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	69	1.000	Domainoid score	I2674
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100256
Panther	1	1.100	-	-	LDO	PTHR11254
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2273
SonicParanoid	1	1.000	-	-		X2041
TreeFam	0	0.000	Not matched by this tool.
	8	7.840