DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Smurf and Magi3

DIOPT Version :9

Sequence 1:	NP_001261061.1	Gene:	Smurf / 36999	FlyBaseID:	FBgn0029006	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	NP_620784.3	Gene:	Magi3 / 245903	RGDID:	621362	Length:	1470	Species:	Rattus norvegicus

Alignment Length:	1060	Identity:	207/1060 - (19%)
Similarity:	315/1060 - (29%)	Gaps:	387/1060 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly   112 QRLD--LGKLSPDDD-ELVRGQIIISLLSKDGPSSGNPLAIVGPSGDVRGPSED-------DSSE 166
            :|:|  |.:...|:| |.|.|...:........||...:..| ||.:....|.|       |.:.
  Rat   232 ERMDSSLPEEEEDEDKEAVNGSGSMETREMHSESSDCWMKTV-PSYNQTNRSMDFRNYMMRDENL 295

  Fly   167 DSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPGVVGSSHATSPQQRHNTHNGNSGDRQAPAG 231
            :.||:.||...||.|.:|:::|.||:|.|..||.     ...|.:|:        :..|.:.|.|
  Rat   296 EPLPKNWEMAYTDTGTIYFIDHNTKTTTWLDPRL-----CKKAKAPE--------DCEDGELPYG 347

  Fly   232 ------P----------TRSTTCTNLMNNGHRSRDL------SVTASDERRHSTEILSSVGKENT 274
                  |          .:.|...|.:....|.:.:      |.....||.|.|        .:.
  Rat   348 WEKIEDPQYGTYYVDHLNQKTQFENPVEEAKRKKQIGQAETHSAKTDVERAHFT--------RDP 404

  Fly   275 SPTTPVSATTTPGKKTSSSNSSSAGGRTLEQRP----------TNEPATPTSSTTSASVRLHSND 329
            |....|....:..|.|.....:..||    .||          .:.||..........|.:..|.
  Rat   405 SQLKGVLVRASLKKSTMGFGFTIIGG----DRPDEFLQVKNVLKDGPAAQDGKMAPGDVIVDING 465

  Fly   330 NHVKTPKHQTNGHAPPEST---PTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQSASNGWTQEDA 391
            |.|.       ||...:..   ...|..|  |||.....|
  Rat   466 NCVL-------GHTHADVVQMFQLVPVNQ--YVNLTLCRG------------------------- 496

  Fly   392 ATTTSPSTTTSPPRHSQSPPTPNISPPASVTPSANGNVHSPNANSTPAGSGGGSRSYTAATPGQR 456
                     .:.|..|:.|    :....:.||..||
  Rat   497 ---------YALPDDSEDP----VVDIVAATPVING----------------------------- 519

  Fly   457 SQRRSSRQQGEESSTRRRSSRGTR---NGGTSGG-------GGGGGSGQRYASAAIAAANQ---A 508
                .|..:||...:.:....|..   ..|.||.       .|...|.::.||.|.:.::|   .
  Rat   520 ----QSLAKGEACMSTQDFKLGAMVLDQNGKSGKLLSSDHLNGPSDSNEQRASLASSGSSQPELV 580

  Fly   509 ARPFLDLPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIPRDFDTQHLTLDAIGPLPSGW---EQR 570
            ..|.:..|.|:.......        |||      .::....|:|             |   .|:
  Rat   581 TIPLVKGPKGFGFAIADS--------PTG------QKVKMILDSQ-------------WCQGLQK 618

  Fly   571 KTASGRVYFVDHNNRT--------TQFTDPRLSGSILQMIRRGTVPPTSAA-------------- 613
            ......:|..:..|.|        .||  |..:...|.::|.|...||..|
  Rat   619 GDIIKEIYHQNVQNLTHLQVVEVLKQF--PVGADVPLLILRGGPCSPTKTAKMKTDTKETSGSLE 681

  Fly   614 --NAGTPAPPSATPATPSAAAAVPPQATPASNATPTTLTTTTNPPH-----------------RI 659
              |..||.|   .|..||...:..|:..|:.....:.......||:                 |:
  Rat   682 TINEPTPQP---MPFPPSIIRSGSPKLDPSEVYLKSKTLYEDKPPNTKDLDVFLRKQESGFGFRV 743

  Fly   660 V----PDLPQGLLEGADLLPKYRRDLVGKLRALR------------------TELQTMQPQSGHC 702
            :    ||  |.:..|| ::|....:..|:|||..                  .:|.|...::||.
  Rat   744 LGGDGPD--QSIYIGA-IIPLGAAEKDGRLRAADELMCIDGIPVKGKSHKQVLDLMTTAARNGHV 805

  Fly   703 RLEVSRNEIF-------EESYRLIMKMRAKDM-RKRLMVKFKGEEGLDYGGVAREWLHLLSREML 759
            .|.| |.:||       :||.:...:..:..: |..|..:...:|..|.         :|.|:.
  Rat   806 LLTV-RRKIFYGEKQPEDESPQAFSQSGSPRLNRTELPTRSAPQESYDV---------ILQRKE- 859

  Fly   760 NPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNK 824
            |..:|..      ..|.:..|..||.|      |.:||.          :||            .
  Rat   860 NEGFGFV------ILTSKSKPPPGVIP------HKIGRV----------IDG------------S 890

  Fly   825 P------ITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPGGASIPVT 883
            |      :.:||        |.|..     |...|::  .|.:|      :|..:|..|.::.:|
  Rat   891 PADRCGRLKVGD--------HISAV-----NGQSIVD--LSHDN------IVQLIKDAGVTVTLT 934

  Fly   884 EENKREYVKLYVNYRFMRG------IEQQFLALQ-KGFCELIPSHLLRPFDERELELVIG-GISS 940
            ...:.|:          .|      ..:|..||| :...:...:|:  |.|...||..:| .:.|
  Rat   935 VVAEEEH----------HGPPSGTNSARQSPALQHRPMGQAQATHI--PGDRTALEGEVGKDVCS 987

  Fly   941 IDVNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQ------GFRALQ 999
            ...:.|.::   ||.....|.|:   .||.|..|:.        .|...|.|:      ||....
  Rat   988 SYRHSWSDH---KHLAQPDTAVI---SVVGSRHSQS--------LGCYPVELERGPRGFGFSLRG 1038

  Fly  1000 GSTGAVGPRLFTIHLTADVP 1019
            |....:|  ||.:.|..|.|
  Rat  1039 GKEYNMG--LFILRLAEDGP 1056

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_001261061.1	C2_Smurf-like	14..135	CDD:176028	8/25 (32%)
		WW	169..198	CDD:278809	13/28 (46%)
		WW	514..546	CDD:197736	5/31 (16%)
		WW	562..594	CDD:197736	8/42 (19%)
		HECTc	702..1058	CDD:238033	72/346 (21%)
		HECTc	726..1058	CDD:214523	65/315 (21%)
Magi3	NP_620784.3	Interaction with ADRB1 and TGFA. /evidence=ECO:0000269\|PubMed:16316992	18..108
		PDZ_signaling	24..103	CDD:238492
		NK	125..>199	CDD:418433
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	184..266		8/33 (24%)
		HUL4	<211..>373	CDD:227354	38/154 (25%)
		WW	299..329	CDD:238122	13/29 (45%)
		WW	345..375	CDD:238122	5/29 (17%)
		Interaction with PTEN. /evidence=ECO:0000250	413..495		19/94 (20%)
		PDZ	416..496	CDD:214570	19/92 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	550..575		5/24 (21%)
		PDZ	578..659	CDD:214570	18/109 (17%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	665..700		8/37 (22%)
		PDZ	729..812	CDD:214570	18/86 (21%)
		Interaction with ADGRB1. /evidence=ECO:0000250	729..811		17/85 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	818..847		4/28 (14%)
		PDZ_signaling	851..936	CDD:238492	28/149 (19%)
		Interaction with LPAR2 and GRIN2B. /evidence=ECO:0000250	852..939		28/151 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	939..976		9/48 (19%)
		PDZ_signaling	1023..1101	CDD:238492	11/36 (31%)
		PTZ00112	1115..>1405	CDD:240274
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1124..1146
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1167..1470
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900