Protein Alignment Smurf and Itch

DIOPT Version :10

• Sequence 1: NP_523779.1
• Gene: Smurf / 36999
• FlyBaseID: FBgn0029006
• Length: 1061
• Species: Drosophila melanogaster
• Sequence 2: NP_032421.2
• Gene: Itch / 16396
• MGIID: 1202301
• Length: 864
• Species: Mus musculus
• Alignment Length: 835
• Identity: 296/835 - (35%)
• Similarity: 406/835 - (48%)
• Gaps: 198/835 - (23%)

  Fly   310 EPATPTSSTTSASVRLHSNDNHVKT---PKHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGN 371
            |....|:..||.|.....||:..:|   .:..|||...||            |..:.:|...:||
Mouse   145 EAEVVTNGETSCSESTTQNDDGCRTRDDTRVSTNGSEDPE------------VAASGENKRANGN 197

  Fly   372 GSGQAAQPQSASNGWTQEDAATTTSPSTTTSPPRHSQSPPTPNISPPASVTPSANGNVHSPNANS 436
            .|      .|.|||..:        ||   .|||.|: ||.|....||||    ||   ||:.||
Mouse   198 NS------PSLSNGGFK--------PS---RPPRPSR-PPPPTPRRPASV----NG---SPSTNS 237

  Fly   437 TPAGSGGGSRSYTAATPGQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSG----GGGGGGSGQRY 497
            ...||..||.                      ..|....:..|..|.|||    ....||||.|
Mouse   238 DSDGSSTGSL----------------------PPTNTNVNTSTSEGATSGLIIPLTISGGSGPR- 279

  Fly   498 ASAAIAAANQAARP--------------FLD-------------LPPGYEMRTTQQGQVYFYHIP 535
               .:...:||..|              ::|             ||||:|.|....|::|:....
Mouse   280 ---PLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHF 341

  Fly   536 TGVSTWHDPRIP--RDFD------------------------------TQHLTLDAIGPLPSGWE 568
            |..:||..|.:.  |:::                              :|:...|.:||||.|||
Mouse   342 TRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIYGNQDLFATSQNKEFDPLGPLPPGWE 406

  Fly   569 QRKTASGRVYFVDHNNRTTQFTDPRLSGSILQ------MIRRGTVPPTSAANAGTP--------A 619
            :|..::||||||:||.|.||:.|||..|.:.:      ...|.||.       |.|        |
Mouse   407 KRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVD-------GIPYFVDHNRRA 464

  Fly   620 PPSATPATPSAAAAVPPQATPASNATPTTLTTTTNPPHRIVPDLPQGLLEGADLLPKYRRDLVGK 684
            .....|.|..:|....||..                                     |.||...|
Mouse   465 TTYIDPRTGKSALDNGPQIA-------------------------------------YVRDFKAK 492

  Fly   685 LRALRTELQTM-QPQSGHCRLEVSRNEIFEESYRLIMKMRAKDMRKRLMVKFKGEEGLDYGGVAR 748
            ::..|...|.: .||  |.::.|:|..:||:|::.||....:|:|:||.|.|.||||||||||||
Mouse   493 VQYFRFWCQQLAMPQ--HIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAR 555

  Fly   749 EWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDGGF 813
            ||..|||.|:|||.|.||:|:..|:|.|||||.|.:|||||.||.|:||.:.:|:|||..:|.||
Mouse   556 EWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGF 620

  Fly   814 TTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGI-IESTFSVENNSFGALVVHELKPGG 877
            :.||||::||||:.|.|:|.:||:.:.||.|:.|:||... :|..|||:....|.:..|:|||.|
Mouse   621 SLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNG 685

  Fly   878 ASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSID 942
            .:|.||||||.||:::...:|..||:|:|..|..:||.|::|...|:.||.:|||:::.|:..||
Mouse   686 GNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEID 750

  Fly   943 VNDWRNNTRLKHCTNETTQVLWFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAVGP 1007
            :|||:.:...:|.|..:.|::||||.|:...:|.|.||||||||:.|:|:.||..|.||.   ||
Mouse   751 LNDWQRHAIYRHYTRTSKQIMWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSN---GP 812

  Fly  1008 RLFTIHLTADVPTQN-LPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061
            :.|.|.   .|..:| ||::||||||:|||||::|:.|.:||..|:|||.||..|
Mouse   813 QKFCIE---KVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE 864

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_523779.1	C2_Smurf-like	14..135	CDD:176028
		PRP40	155..>283	CDD:227435
		WW	169..198	CDD:459800
		WW	514..546	CDD:197736	13/44 (30%)
		WW	562..594	CDD:197736	20/31 (65%)
		HECTc	702..1058	CDD:238033	175/357 (49%)
Itch	NP_032421.2	C2_E3_ubiquitin_ligase	17..140	CDD:175988
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	151..294		57/205 (28%)
		WW	289..318	CDD:459800	2/28 (7%)
		HUL4	<320..864	CDD:227354	235/595 (39%)
		Required for interaction with FYN. /evidence=ECO:0000250	356..432		24/75 (32%)
		MAP kinase docking site	535..544		5/8 (63%)