Protein Alignment Smurf and hecw2b

DIOPT Version :10

• Sequence 1: NP_523779.1
• Gene: Smurf / 36999
• FlyBaseID: FBgn0029006
• Length: 1061
• Species: Drosophila melanogaster
• Sequence 2: XP_003197660.3
• Gene: hecw2b / 100331355
• ZFINID: ZDB-GENE-101001-3
• Length: 1472
• Species: Danio rerio
• Alignment Length: 1382
• Identity: 360/1382 - (26%)
• Similarity: 517/1382 - (37%)
• Gaps: 467/1382 - (33%)

  Fly    27 RKDLFRLPDPFAKVQV-----------DGTGQVYSTEISKSSLDPKWNAHYDLFLGI-GDAITIT 79
            :|.:|..|||:.|:.:           ...||...|.|..::.:|.|:.....|:.: .|.:.|.
Zfish   211 KKGMFFNPDPYLKMSIQPGKRNGFPTFSHHGQERRTSIISNTTNPVWHGEKYTFVALMTDVLEIE 275

  Fly    80 VWNQ----RKIHKGSGFLG------------------------CVRIP--------------AFN 102
            |.::    |.|.|  .|||                        |.|:|              .||
Zfish   276 VKDKFAKSRPIIK--RFLGQLIIPVQRLLERHTAGDQHVSYTLCRRLPTEHVSGQLHFRVEITFN 338

  Fly   103 -----IQSLKGAGFQRLDLGKLSPDDDE--------LVRGQIIISLLSKDGPSSGNPLAIVGPSG 154
                 :.||.||.....|.|  ||.|||        :|||.   |....:|...|:...   |..
Zfish   339 GHEDAMGSLLGASAMNGDPG--SPSDDEDVLQPVSRMVRGP---SPTGSEGSLHGDAFR---PDE 395

  Fly   155 DVRGPSEDDSSEDSLPEGWEERRTDNGRVYYVNHATKSTQWDRPRQPG--VVGSSHATSPQQRHN 217
            .:..|.||....|..|:..|.......|...:|....:.  :.|:.||  .:|   |:||:.|.:
Zfish   396 CMMDPEEDRLLRDGAPDLAEGAPAHTHRQSSLNDYLDAI--EAPKPPGDRPLG---ASSPKLRSS 455

  Fly   218 ----------THNGNSGDRQAPAGPTRSTTCTNLM--NNGHRSRDL---SVTASDERRHSTEILS 267
                      .|..:..|.:..|  :|.|..||.|  ..||.|.:.   .|...:..|..:|..|
Zfish   456 FPTDTRLNAMLHIDSDEDEEGHA--SRDTALTNGMPRPEGHASSERGKGEVHVKNRERLESEAAS 518

  Fly   268 SVGKENTSPTTPVSATTTPGKKTSSSNSSSA----------GGRTLEQRPTNEPATPTSSTTSAS 322
            |.  .:|..|..||::.|    |.|.|...|          ||.|.......:||
Zfish   519 SA--TDTEATASVSSSGT----TKSQNGGGAEGGQEGAAISGGLTQSISSFQQPA---------- 567

  Fly   323 VRLHSNDNHVKTPKHQTNGHAPPESTPTSPTGQQNYVNGNAQNGSTSGNGSGQAAQPQSASNGWT 387
            :|:...:           |.||..|:                  .|.|:....|....|||..:|
Zfish   568 LRIQDEE-----------GAAPQASS------------------RTEGSSEAPAVSEASASAIYT 603

  Fly   388 QEDAATTTSPSTTTSPPRHSQSPPTPNISPPASVTPSANGNVHSPNANSTPAGSGGGSRSYTAAT 452
            ..:....|:   |..|.|.:.|..|
Zfish   604 VNEVGGATA---TNQPERMNSSEMT---------------------------------------- 625

  Fly   453 PGQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSGGGGGGGSGQRYASAAIAA------------- 504
                    :..::.||...||||.:.:.|...:.....|...:|.|..|.||
Zfish   626 --------NQEEEDEEIWQRRRSLQASGNESQAEAMELGNEVRRTADEAQAALITDGETGACAQV 682

  Fly   505 -ANQAARPF------------LD--LPPGYEMRTTQQGQVYFYHIPTGVSTWHDPRIP------- 547
             .:|..|..            :|  |||.:|.|....|::::.......:||..|..|
Zfish   683 NGHQPMRSLPSVRHDISRYQRVDEPLPPNWEARIDSHGRIFYVDHVNRTTTWQRPTAPPAPQVLQ 747

  Fly   548 ------------RDFDTQHLTL---------------------------------DAIGP----- 562
                        |.:.:...|:                                 ..:||
Zfish   748 RSNSIQQMEQLNRRYQSIRRTITNERTEEQAAEMPPDDLTTSDLLHHSIPEYRRDGVVGPASSRS 812

  Fly   563 ----------------------------------------------------------------- 562
                                                                             
Zfish   813 RLTLLLQSPSAKFLTSPDFFTVLHSNPSAYRMFTSNTCLKHMISKVRRDAHHFERYQHNRDLVAF 877

  Fly   563 ----------LPSGWEQRKTASGRVYFVDHNNRTTQFTDPRL------SGSIL---QMIRR---- 604
                      ||.|||.:...:|:.:|||||.|.|.|.||||      ..|:|   |.:.|
Zfish   878 LNMFANKQLDLPRGWEMKHDHTGKPFFVDHNCRATTFIDPRLPLQSARPSSLLAHRQHLSRQRSH 942

  Fly   605 --GTV--PPTSAANAGTPAPPSATPATPSAAAAVPPQA---------------------TP---- 640
              |.|  .|..|..|..|.|.:...|..:....:.|.|                     .|
Zfish   943 SAGEVGDDPRHAGPAVLPRPSNTFTANRNQCQDLVPVAYNEKIVAFLRQPNIFDILQERQPELIR 1007

  Fly   641 ----------------------ASNATPTTLTTTTN-------PPHRIV---------------P 661
                                  :|:|....|.:...       |||.::               |
Zfish  1008 NHSLREKVQFIRNEGASGLARLSSDADLVILLSLFEEEVMSYVPPHALLHPSYCQSPRGSPVSSP 1072

  Fly   662 DLPQGLLEGADLLP-KYRRDLVGKLRALRTELQT----MQPQSGHCRLEVSRNEIFEESYRLIMK 721
            ....|........| .|:||...|||....:|:|    ..|  |..:|.:.|:.:.|:::..||.
Zfish  1073 QNSPGTQRANARAPAPYKRDFEAKLRNFYRKLETKGYGQGP--GKVKLIIRRDHLLEDAFNQIMC 1135

  Fly   722 MRAKDM-RKRLMVKFKGEEGLDYGGVAREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVN 785
            ...||: |.:|.|.|.|||||||.|.:||:..|:|||:.||.||||:||.:|.||:||:|.|...
Zfish  1136 YSRKDLQRSKLYVSFVGEEGLDYSGPSREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFV 1200

  Fly   786 PDHLSYFHFVGRTLGIAVFHGHCLDGGFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNI 850
            .:|..:|.|.||.||:|:.|.:.||..||.||||.||..|..|.|:|.:|.:.|:||.||.:::|
Zfish  1201 DNHHEWFRFSGRILGLALIHQYLLDAFFTRPFYKGLLRIPCDLSDLEYLDEEFHQSLQWMKDNDI 1265

  Fly   851 SGIIESTFSVENNSFGALVVHELKPGGASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFC 915
            ..:::.||:|....||.:...|||||||:|||:|:||:||::..|.:|..||:.||..:|.:||.
Zfish  1266 EDMLDLTFTVNEEVFGQITERELKPGGANIPVSEKNKKEYIERMVKWRIERGVVQQTESLVRGFY 1330

  Fly   916 ELIPSHLLRPFDERELELVIGGISSIDVNDWRNNTRLKHCTNETTQVL-WFWQVVESYSSEMRAR 979
            |::.:.|:..||.|||||||.|.:.||:.||||||..:...::...|: |||..||.:::|.|.|
Zfish  1331 EVVDARLVSVFDARELELVIAGTAEIDLGDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLR 1395

  Fly   980 LLQFVTGSSRVPLQGFRALQGSTGAVGPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLL 1044
            |||||||:|.:|.:||.:|:||.   |||.|.:.....:  .:||:|||||||:|||||.::.:|
Zfish  1396 LLQFVTGTSSIPYEGFASLRGSN---GPRRFCVEKWGKI--TSLPRAHTCFNRLDLPPYPSFSML 1455

  Fly  1045 CDKLTQAVEETCGFAVE 1061
            .:|:..|||||..|.:|
Zfish  1456 YEKMLTAVEETSTFGLE 1472

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_523779.1	C2_Smurf-like	14..135	CDD:176028	41/174 (24%)
		PRP40	155..>283	CDD:227435	36/144 (25%)
		WW	169..198	CDD:459800	4/28 (14%)
		WW	514..546	CDD:197736	10/33 (30%)
		WW	562..594	CDD:197736	17/111 (15%)
		HECTc	702..1058	CDD:238033	167/357 (47%)
hecw2b	XP_003197660.3	HECW_N	58..175	CDD:465177
		C2_NEDL1-like	198..334	CDD:176073	25/124 (20%)
		LGT	<491..633	CDD:469786	40/237 (17%)
		WW	708..737	CDD:459800	8/28 (29%)
		HECW1_helix	816..882	CDD:465766	0/65 (0%)
		WW	887..919	CDD:197736	16/31 (52%)
		HECTc	1117..1470	CDD:238033	167/357 (47%)