DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Smurf and hecw2a

DIOPT Version :9

Sequence 1:	NP_001261061.1	Gene:	Smurf / 36999	FlyBaseID:	FBgn0029006	Length:	1061	Species:	Drosophila melanogaster
Sequence 2:	XP_009302976.1	Gene:	hecw2a / 100150269	ZFINID:	ZDB-GENE-090311-22	Length:	1475	Species:	Danio rerio

Alignment Length:	1356	Identity:	354/1356 - (26%)
Similarity:	521/1356 - (38%)	Gaps:	402/1356 - (29%)

- Green bases have known domain annotations that are detailed below.

  Fly    27 RKDLFRLPDPFAKVQV-----------DGTGQVYSTEISKSSLDPKWNAHYDLFLGI-GDAITIT 79
            :|.:|..|||:.|:.:           ...||...:.|:.::.:|.|:.....|:.: .|.:.|.
Zfish   201 KKGMFFNPDPYLKMSIHPGKRSCFPTFSHHGQERRSGITTNTTNPVWHGEKHTFVALMTDVLVIE 265

  Fly    80 VWNQ----RKIHKGSGFLGCVRIPAFNIQSLKGAGFQ---------------------RLDLGKL 119
            |.::    |.|.|  .|||.:.||...:......|.|                     ::|:..:
Zfish   266 VKDKFAKSRPIIK--RFLGQLNIPVQLLLERHSTGNQSLSFSLCRRLPTDHVSGQMHFKVDVTSM 328

  Fly   120 SPDD--DELVRGQIIIS----------------LLSKDGPS---------SGNPLAIVGPSGDVR 157
            .|||  .|.:.|...::                .:|..|||         :|...:|..|..::.
Zfish   329 GPDDVSPETILGAAALNGAPGTPSDDEELPHPLPISSAGPSPTASLGSNRNGEGSSIPSPDTEIY 393

  Fly   158 GPSEDD---SSEDSLPEGWEERRTDNGRVYYVNHATKSTQWD---RPRQPG--VVGSSHATSPQQ 214
            |.:.||   ||.|.|...:.|                  |.|   .|:.||  .:|   |.||:.
Zfish   394 GGTLDDEAPSSPDLLQGSFSE------------------QLDAIEAPKGPGDRPLG---AASPKL 437

  Fly   215 RHN--THNGNSG----------DRQAPAGPTRSTTCTNLMNNGHRSRDLSVTA------------ 255
            |.:  ||...|.          ||.|....|.||     .|...||......|
Zfish   438 RSSFPTHTRLSAMLHIDSDEDEDRSAATEATLST-----FNGIPRSYQTPPKAAARGAVFDGGIE 497

  Fly   256 --SDERRHSTEILS-SVGKENTSPTTPVSATTTPGKKTSS-SNSSSAGGRTLEQRPTNEPATPTS 316
              |:|....:|:.: :.|:|.......|.::..|..:|:| .......||.:|.....|.....|
Zfish   498 EPSEEAGLESEVETLNAGEEVPPEPEVVPSSVAPEVETASLMERQEEQGREVEVEEEEEEGLARS 562

  Fly   317 ------------------STTSASVRLHSNDNHVKTPKHQTNGHAPPESTPTSPTGQQNYVNGNA 363
                              ...|||............|..:.....||.:...:.|.::.::....
Zfish   563 EELATEVDISSMASDCCPGPASASQEAEQGAEAAIDPGGEDLSDDPPTTYEVAETAEETHLTNEP 627

  Fly   364 QNGSTSGNGSGQAAQPQSASN-----------------------GWTQEDA--------ATTTSP 397
            :.         :|.:|:..:|                       |.:|||.        ..:...
Zfish   628 ER---------EAPKPEPPTNEEEECEDVTEDTMSRRWSLEATAGVSQEDEDELIPSGDGESVDS 683

  Fly   398 STTTSPPRHSQSPPTPNISPPASVTPSANGNVH---------SPNANSTPAGSG------GGSRS 447
            .|..:........|..|.....:..||...::|         .||..:.....|      ..:|:
Zfish   684 ETFVTETESELGVPQVNGDQRVNSLPSVRQDIHRYQRVDEPLPPNWEARIDSHGRIFFVDHVNRT 748

  Fly   448 YTAATP-----GQRSQRRSSRQQGEESSTRRRSSRGTRNGGTSGGGGGGGSGQRYASAAIAAANQ 507
            .|...|     .|...|.||..|.|:.:.|.:|.|.|                       ..:.:
Zfish   749 TTWQRPTGPPAPQGLTRSSSILQMEQLNRRYQSIRRT-----------------------MTSER 790

  Fly   508 AARPFLDLPPGYEMRTTQQ-----------GQ---------------VYFYHIPTGVSTWH-DPR 545
            :..|.::.||..|.....|           ||               ..|...|...|..| :|.
Zfish   791 SEEPRVEDPPADETDVLAQSISEYRRDGIIGQSSSRSRLALLLQSPGAKFLSSPDFFSVLHSNPS 855

  Fly   546 IPRDF---------------DTQHL-----TLDAIG----------PLPSGWEQRKTASGRVYFV 580
            ..|.|               |.||.     ..|.:.          .||.|||.:...:|:.:||
Zfish   856 AYRMFTSNTCLKHMISKVRRDAQHFERYQHNRDLVNFLNLFSNKQLELPRGWEMKHDHTGKAFFV 920

  Fly   581 DHNNRTTQFTDPRL-------SGSILQMIRRGTVPPTSAANAG----TPAPP------------- 621
            |||.|.|.|.||||       ||.:............||...|    :|:||
Zfish   921 DHNCRATTFIDPRLPLQNSRSSGLLAHRQHLSRQRSHSAGEVGDDSRSPSPPVQGRSSRNSQYQD 985

  Fly   622 ----------------------------------------------SATPATPSAAAA------- 633
                                                          .||..|..::.|
Zfish   986 LVPVAYNDKIVAFLRQPNIFEILQERQPELVRNHSLREKVQFIRNEGATGLTRLSSDADLVMLLS 1050

  Fly   634 ---------VPPQATPAS---NATPTTLTTTTNPPHRIVPDLPQGLLEGADLLPKYRRDLVGKLR 686
                     |||.|...|   :|:|.:           .|..|:.   .|.....|:||...|||
Zfish  1051 LFEEEVMSYVPPNALLLSSYCHASPQS-----------SPGTPRA---NARAPAPYKRDFEAKLR 1101

  Fly   687 ALRTELQT----MQPQSGHCRLEVSRNEIFEESYRLIMKMRAKDM-RKRLMVKFKGEEGLDYGGV 746
            ....:::|    ..|  |..:|.:.|:.:.|:::..||....||: |.||.|.|.|||||||.|.
Zfish  1102 NFYRKMETKGYGQGP--GKVKLIIRRDHLLEDAFNQIMCYSRKDLQRSRLYVSFVGEEGLDYSGP 1164

  Fly   747 AREWLHLLSREMLNPQYGLFQYSRDDHYTLQINPDSGVNPDHLSYFHFVGRTLGIAVFHGHCLDG 811
            :||:..|:|||:.||.||||:||.:|.||:||:|.|....:|..:|.|.||.||:|:.|.:.||.
Zfish  1165 SREFFFLVSRELFNPYYGLFEYSANDTYTVQISPMSAFVDNHHEWFRFSGRILGLALIHQYLLDA 1229

  Fly   812 GFTTPFYKQLLNKPITLGDIEGVDPDLHRSLTWMLESNISGIIESTFSVENNSFGALVVHELKPG 876
            .||.||||.||..|..|.|:|.:|.:.|:||.||.:::|..:::.||:|....||.:...|||||
Zfish  1230 FFTRPFYKGLLRIPCDLSDLEFLDEEFHQSLQWMKDNDIEDMLDLTFTVNEEVFGQITERELKPG 1294

  Fly   877 GASIPVTEENKREYVKLYVNYRFMRGIEQQFLALQKGFCELIPSHLLRPFDERELELVIGGISSI 941
            |:.|.|:::||:||::..|.:|..||:.||..:|.:||.|::...|:..||.|||||||.|.:.|
Zfish  1295 GSGIAVSDKNKKEYIERMVKWRIERGVAQQTESLVRGFYEVVDVRLVSVFDARELELVIAGTAEI 1359

  Fly   942 DVNDWRNNTRLKHCTNETTQVL-WFWQVVESYSSEMRARLLQFVTGSSRVPLQGFRALQGSTGAV 1005
            |::||||||..:...::...|: |||..||.:::|.|.||||||||:|.:|.:||.:|:||.
Zfish  1360 DLSDWRNNTEYRGGYHDNHIVIRWFWAAVERFNNEQRLRLLQFVTGTSSIPYEGFASLRGSN--- 1421

  Fly  1006 GPRLFTIHLTADVPTQNLPKAHTCFNRIDLPPYETYQLLCDKLTQAVEETCGFAVE 1061
            |||.|.:.....|  .:||:|||||||:|||||.::.:|.:|:..|||||..|.:|
Zfish  1422 GPRRFCVEKWGKV--TSLPRAHTCFNRLDLPPYPSFSMLYEKMVTAVEETSTFGLE 1475

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Smurf	NP_001261061.1	C2_Smurf-like	14..135	CDD:176028	31/162 (19%)
		WW	169..198	CDD:278809	4/31 (13%)
		WW	514..546	CDD:197736	11/58 (19%)
		WW	562..594	CDD:197736	16/31 (52%)
		HECTc	702..1058	CDD:238033	166/357 (46%)
		HECTc	726..1058	CDD:214523	160/333 (48%)
hecw2a	XP_009302976.1	HECW_N	50..169	CDD:318711
		C2_NEDL1-like	188..323	CDD:176073	25/123 (20%)
		WW	724..756	CDD:197736	6/31 (19%)
		WW	903..934	CDD:197736	16/30 (53%)
		HECTc	1120..1473	CDD:238033	166/357 (46%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.810