Protein Alignment Dcr-2 and DCL4

DIOPT Version :10

• Sequence 1: NP_523778.2
• Gene: Dcr-2 / 36993
• FlyBaseID: FBgn0034246
• Length: 1722
• Species: Drosophila melanogaster
• Sequence 2: NP_197532.3
• Gene: DCL4 / 832154
• AraportID: AT5G20320
• Length: 1702
• Species: Arabidopsis thaliana
• Alignment Length: 1796
• Identity: 402/1796 - (22%)
• Similarity: 687/1796 - (38%)
• Gaps: 481/1796 - (26%)

  Fly     9 RGYQLRLVDHLTKSNGIVYLPTGSGKTFVAILVLKRFSQDFDKPIESGGKRALFMCNTVELARQQ 73
            |.||:.|....|:.|.||||.||.|||.:|::::.........|.:|   ..:|:..||.|..||
plant   125 RRYQVELCKKATEENVIVYLGTGCGKTHIAVMLIYELGHLVLSPKKS---VCIFLAPTVALVEQQ 186

  Fly    74 AMAVRRCTNFKVGFYVGEQGVDDWTRGMWSDEIKKNQVLVGTAQVFLDMVTQTYVALSSLSVVII 138
            |..:....||||..:.|.:.:.. :...|..||..|:|||.|.|:.|..:...::.:..:|::|.
plant   187 AKVIADSVNFKVAIHCGGKRIVK-SHSEWEREIAANEVLVMTPQILLHNLQHCFIKMECISLLIF 250

  Fly   139 DECHHG--TGHHPFREFMRLFTIANQTKLPRVVGLTG--VLIKGN-EITNVATKLKELEITYRGN 198
            |||||.  ..:||:.|.|::|..:...:.||:.|:|.  |:.||: :..|::..:..||......
plant   251 DECHHAQQQSNHPYAEIMKVFYKSESLQRPRIFGMTASPVVGKGSFQSENLSKSINSLENLLNAK 315

  Fly   199 IITVSDTKEMENVMLYATKPTEVMVSFPHQEQVLTVTRLISAEIEKFYVSLDLMNIGVQPIRRSK 263
            :.:|....:::.           .||.|              .::.:|....|.:.....||...
plant   316 VYSVESNVQLDG-----------FVSSP--------------LVKVYYYRSALSDASQSTIRYEN 355

  Fly   264 SLQ-----CLRD-------------PSKKSFVKQLFNDFLYQMKEYGIYAA-SIAIISLIVEFDI 309
            .|:     ||..             .|.|..:|:..::.:|.:...|::.| ..|.|.|..:.::
plant   356 MLEDIKQRCLASLKLLIDTHQTQTLLSMKRLLKRSHDNLIYTLLNLGLWGAIQAAKIQLNSDHNV 420

  Fly   310 KRRQAETLSVKLMHRTALTLCEKIRHLLVQKLQDMTYDDDDDNVNTEEVIMNFSTPKVQRFLMSL 374
               |.|.:......:    :|:....:..:.|....  ..|:|.:....:.....|...|.|:.|
plant   421 ---QDEPVGKNPKSK----ICDTYLSMAAEALSSGV--AKDENASDLLSLAALKEPLFSRKLVQL 476

  Fly   375 -KVSFADKDPKDICCLVFVERRYTCKCIYGLLLNYIQSTPELRNVLTPQFMVGRNNISPDFESVL 438
             |:....:....:.|::||.|..|.:.:..:|.|.     ||.......|:||   :|...:| :
plant   477 IKILSVFRLEPHMKCIIFVNRIVTARTLSCILNNL-----ELLRSWKSDFLVG---LSSGLKS-M 532

  Fly   439 ERKWQKSAIQQFRDGNANLMICSSVLEEGIDVQACNHVFILDPVKTFNMYVQSKGRARTTEAKFV 503
            .|:..::.:::|:....||::.:.|.|||:|:|.|..|...|..:|...::||:||||..::::.
plant   533 SRRSMETILKRFQSKELNLLVATKVGEEGLDIQTCCLVIRYDLPETVTSFIQSRGRARMPQSEYA 597

  Fly   504 LFTADKEREKTIQQIYQYRKAHNDIAEYLKDRVLEKTEP----ELYEIKGHFQDDIDPFTNENGA 564
             |..|...||.:..|..::...:.:...:..|..|:|.|    |||::            :|.||
plant   598 -FLVDSGNEKEMDLIENFKVNEDRMNLEITYRSSEETCPRLDEELYKV------------HETGA 649

  Fly   565 VLLPNNALAILHRYCQTIPTDAFGFVIPWFHVLQEDERDRIFGVSAKGKHVISINMPVNCMLRDT 629
            .:...:::::|::||..:|.|.|....|.|.....||    ||.:     :..|.:|.|..:.:
plant   650 CISGGSSISLLYKYCSRLPHDEFFQPKPEFQFKPVDE----FGGT-----ICRITLPANAPISE- 704

  Fly   630 IYSDPMDNVKTAKISAAFKACKVLYSLGELNERFVPKTLKERVASIADVHFEHWNKYGDSVTATV 694
            |.|..:.:.:.||..|..||...|::||.||:..:|.:..|....::|..|:..|..|:..
plant   705 IESSLLPSTEAAKKDACLKAVHELHNLGVLNDFLLPDSKDEIEDELSDDEFDFDNIKGEGC---- 765

  Fly   695 NKADKSKDRTYKTECPLEF---YDALPRVGEI-CYAYEIFLEPQFESCEYTEHMYLNLQTPRNYA 755
                 |:...|:...|:.|   :|  |....: .::|.|...|     ...:.:|      :.:.
plant   766 -----SRGDLYEMRVPVLFKQKWD--PSTSCVNLHSYYIMFVP-----HPADRIY------KKFG 812

  Fly   756 ILLRNKLPRLAE-MPL---FSNQGKLHVRVANAPLEVIIQNSEQLELLHQFHGMVFR------DI 810
            ..:::.||..|| |.:   .::|..:.|::  .|..|...:::::.|...|..:..:      ::
plant   813 FFMKSPLPVEAETMDIDLHLAHQRSVSVKI--FPSGVTEFDNDEIRLAELFQEIALKVLFERGEL 875

  Fly   811 LKIWHPFFVLD--RRSKENSYLVVPLILGAGEQK-CFDWELMTNFRRLPQSHGSNVQQREQQPAP 872
            :..:.|..:.|  |.||...||::||.|..||.. ..||..:.|....|.....:|...:..|
plant   876 IPDFVPLELQDSSRTSKSTFYLLLPLCLHDGESVISVDWVTIRNCLSSPIFKTPSVLVEDIFP-- 938

  Fly   873 RPEDFEGKIVTQ-WYANYDKPMLVTKVHRELTPLSYMEKNQQDKTYYEFTMSKYGNRIGDVVHKD 936
             |.....|:... |  |.|      .|...|...:|      .|.:|
plant   939 -PSGSHLKLANGCW--NID------DVKNSLVFTTY------SKQFY------------------ 970

  Fly   937 KFMIEVRDLTEQLTFYVHNRGKFNAKSKAKMKVILIPELCFNFNFPGDLWLKLIFLPSILNRMYF 1001
             |:.::          .|.|..|:...::..|
plant   971 -FVADI----------CHGRNGFSPVKESSTK--------------------------------- 991

  Fly  1002 LLHAEALRKRFNTYLNLHLLPFNGTDYMPRPLEIDYSLKRNVDPLGNVIPTEDIEEPKSLLEPMP 1066
             .|.|::.|.:..                       .||....||..|.|.
plant   992 -SHVESIYKLYGV-----------------------ELKHPAQPLLRVKPL-------------- 1018

  Fly  1067 TKSIEASVANLEITEFENPWQKYMEPVDLSRNLLSTYPVELDYYYHFSVGNVCEMNEMDFEDKEY 1131
                 ..|.||    ..|..|..:||.:|....:. .|.||.   |..:..:
plant  1019 -----CHVRNL----LHNRMQTNLEPQELDEYFIE-IPPELS---HLKIKGL------------- 1057

  Fly  1132 WAKNQFHMPTGNIYGNRTPAKTNANVPALMPSKPTVRGKVKPLLILQKTVSKEHITPAEQGEF-- 1194
                               :|...:..:|:||   :..:::.||:   .:..:|:..|...|.
plant  1058 -------------------SKDIGSSLSLLPS---IMHRMENLLV---AIELKHVLSASIPEIAE 1097

  Fly  1195 ------LAAITASSAADVFDMERLEILGDSFLKLSATLYLASKYSDWNEGTLTEVKSKLVSNRNL 1253
                  |.|:|.....:...:||||:|||:|||.:.:.:|...:...:||.||..:|.:|:|.||
plant  1098 VSGHRVLEALTTEKCHERLSLERLEVLGDAFLKFAVSRHLFLHHDSLDEGELTRRRSNVVNNSNL 1162

  Fly  1254 LFCLIDADIPKTLNTIQFTPRYTWLPPGISLPHNVLALWRENPEFAKIIGPHNLRDLALGDEESL 1318
              |.:  .|.|.|...                                     :||.||...:..
plant  1163 --CRL--AIKKNLQVY-------------------------------------IRDQALDPTQFF 1186

  Fly  1319 VKGN-----CSDI------NYNRFVEGCRANGQSFYAGADFSSEVNFCVGLVTIPNKVIADTLEA 1372
            ..|:     |.::      :.||.:....:|          :.|:....|...:..|.|||.:||
plant  1187 AFGHPCRVTCDEVASKEVHSLNRDLGILESN----------TGEIRCSKGHHWLYKKTIADVVEA 1241

  Fly  1373 LLGVIVKNYGLQHAFKMLEYFKICRADIDKPLTQLLNLELGGKK-----MRANVNTTEIDGFLIN 1432
            |:|..:.:.|.:.|.|.|::..:   ::|....|:.:..:..::     .|.|:.|
plant  1242 LVGAFLVDSGFKGAVKFLKWIGV---NVDFESLQVQDACIASRRYLPLTTRNNLET--------- 1294

  Fly  1433 HYYLEKNLGYTFKDRRYLLQALTHPSYPTNRITGS-YQELEFIGDAILDFLISAYIFENNTKMNP 1496
               ||..|.|.|..:..|:||..||||  ||..|. ||.|||:|||:||:|:::|.|....|:.|
plant  1295 ---LENQLDYKFLHKGLLVQAFIHPSY--NRHGGGCYQRLEFLGDAVLDYLMTSYFFTVFPKLKP 1354

  Fly  1497 GALTDLRSALVNNTTLACICVRHRLHFFILAENAKLSEIISKFVNFQESQGHRVTNYVRILLEEA 1561
            |.||||||..|||..||.:.|...|..|:..|:..|.|:|..:.||..|
plant  1355 GQLTDLRSLSVNNEALANVAVSFSLKRFLFCESIYLHEVIEDYTNFLAS---------------- 1403

  Fly  1562 DVQPTPLDLDDELDMTELPHANKCISQEAEKGVPPKGEFNMSTNVDVPKALGDVLEALIAAVYLD 1626
                :||    ....:|.|   :|                       ||.|||::|:.:.|::||
plant  1404 ----SPL----ASGQSEGP---RC-----------------------PKVLGDLVESCLGALFLD 1434

  Fly  1627 CR-DLQRTWEVIFNLFEP-----ELQEFTRKVPINHIRQLVE----HKHAKPVFSSP-----IVE 1676
            |. :|...|.::.:..:|     .||       |:.|::|:|    :|..:.:.::.     .||
plant  1435 CGFNLNHVWTMMLSFLDPVKNLSNLQ-------ISPIKELIELCQSYKWDREISATKKDGAFTVE 1492

  Fly  1677 GETVMVSCQFTCMEKTIKVYGFGSNKDQAKLSAAKHALQQL 1717
            .:.....|..|       |...|.||.:....||:..:..|
plant  1493 LKVTKNGCCLT-------VSATGRNKREGTKKAAQLMITNL 1526

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Dcr-2	NP_523778.2	DEXHc_dicer	6..204	CDD:350792	63/199 (32%)
		Dicer_PBD	241..343	CDD:277191	20/120 (17%)
		SF2_C_dicer	360..506	CDD:350189	39/146 (27%)
		Dicer_dimer	571..670	CDD:460900	28/98 (29%)
		PAZ	843..1004	CDD:198017	21/161 (13%)
		RIBOc	1194..1391	CDD:238333	49/215 (23%)
		RIBOc	1448..1649	CDD:238333	67/207 (32%)
		dsrm	1653..1717	CDD:425434	15/72 (21%)
DCL4	NP_197532.3	DEXHc_dicer	122..321	CDD:350792	63/199 (32%)
		SF2_C_dicer	466..600	CDD:350189	40/143 (28%)
		Dicer_dimer	656..745	CDD:460900	28/98 (29%)
		PAZ	937..1080	CDD:198017	42/307 (14%)
		RIBOc	1104..1261	CDD:238333	50/207 (24%)
		Rnc	1289..1530	CDD:440336	91/316 (29%)
		DSRM_DCL_plant	1627..1697	CDD:380698