Protein Alignment Dcr-2 and DCL3

DIOPT Version :9

• Sequence 1: NP_523778.2
• Gene: Dcr-2 / 36993
• FlyBaseID: FBgn0034246
• Length: 1722
• Species: Drosophila melanogaster
• Sequence 2: NP_001154662.2
• Gene: DCL3 / 823508
• AraportID: AT3G43920
• Length: 1580
• Species: Arabidopsis thaliana
• Alignment Length: 1836
• Identity: 389/1836 - (21%)
• Similarity: 649/1836 - (35%)
• Gaps: 577/1836 - (31%)

  Fly    11 YQLRLVDHLTKSNGIVYLPTGSGKTFVAILVLKRF-SQDFDKPIESGGKRALFMCNTVELARQQA 74
            |:|::.:.....|.|..|.||..|:.:...::|.. |.|.||      :..:|:..||.|.:||.
plant    47 YELKVYEVAKNRNIIAVLGTGIDKSEITKRLIKAMGSSDTDK------RLIIFLAPTVNLVKQQC 105

  Fly    75 MAVRRCTNFKVGFYVGEQGVDDWTRGMWSDEIKKNQVLVGTAQVFLDMVTQTYVALSSLSVVIID 139
            ..:|...|.||..|.|.:|||.||...|.:|..|:.|||.|.|:.||::...::.|..:.::|||
plant   106 CEIRALVNLKVEEYFGAKGVDKWTSQRWDEEFSKHDVLVMTPQILLDVLRSAFLKLEMVCLLIID 170

  Fly   140 ECHHGTGHHPFREFMRLFTIANQTKLPRVVGLT--GVLIKG--NEITNVATKLKELEITYRGNII 200
            ||||.||:||:.:.|:.| ....|..|::.|||  .|:.||  :..:|.|.::.|||......|.
plant   171 ECHHTTGNHPYAKLMKEF-YHESTSKPKIFGLTASAVIRKGIVSSPSNYAAQVSELERLMDSKIF 234

  Fly   201 TVSDTKEMENVMLYATKPTEVMVSF-PHQEQVLTVTRLISAEIEKFYVSLDLMNIGVQPIRRSKS 264
               :.:|.|.|..:||...|..:.: |.....|.    :..::|..::..|            .|
plant   235 ---NPEEREGVEKFATTVKEGPILYNPSPSCSLE----LKEKLETSHLKFD------------AS 280

  Fly   265 LQCLRDPSKKSFV----------KQL---FNDFLYQMKEYGIYAASIAIISLIVEFDIKRRQAET 316
            |:.|::..|.||:          |:|   :.:.|:.:...|:..|.:|....:.:....:.::||
plant   281 LRRLQELGKDSFLNMDNKFETYQKRLSIDYREILHCLDNLGLICAHLAAEVCLEKISDTKEESET 345

  Fly   317 LSVKLMHRTALTLCEKIRHLLVQKLQDMTYDDDDDNVNTEE-----VIMNFSTPKVQRFLMSLKV 376
                  ::....:|::....::..:......||...|:.::     ||....:||::.....|..
plant   346 ------YKECSMVCKEFLEDILSTIGVYLPQDDKSLVDLQQNHLSAVISGHVSPKLKELFHLLDS 404

  Fly   377 SFADKDPKDICCLVFVERRYTCKCIYGLLLNYIQSTPELR--NVLTPQFMVGRNNISPDFESVLE 439
            ...||..:   ||:.|||..|.|.|.    .:::....|.  |||    .:..||.|   .:|..
plant   405 FRGDKQKQ---CLILVERIITAKVIE----RFVKKEASLAYLNVL----YLTENNPS---TNVSA 455

  Fly   440 RKWQKSAIQQFRDGNANLMICSSVLEEGIDVQACNHVFILDPVKTFNMYVQSKGRARTTEAKFVL 504
            :|.|......|:.|..||:..:.|:|||..|..|:.:...|..||...|.||:..|:.:.:|.::
plant   456 QKMQIEIPDLFQHGKVNLLFITDVVEEGFQVPDCSCMVCFDLPKTMCSYSQSQKHAKQSNSKSIM 520

  Fly   505 FTADKEREKTIQQIYQYRKAHNDIAEYLKDRVLEKTEPELYEIK--------GHFQDDIDPFTNE 561
            |.   ||...        |..:.:.:.::..||.: :||...:|        ||...:|
plant   521 FL---ERGNP--------KQRDHLHDLMRREVLIQ-DPEAPNLKSCPPPVKNGHGVKEI------ 567

  Fly   562 NGAVLLPNNALAILHRYCQTIPTDAFGFVIPWFHVLQEDERDRIFGVSAKGKHVISINMPVNCML 626
             |::::|::.:.:......|
plant   568 -GSMVIPDSNITVSEEAAST--------------------------------------------- 586

  Fly   627 RDTIYSDPMDNVKTAKISAAFKACKVLYSLGELNERFVPKTLKERVASIADVHFEHWNKYGDSVT 691
             .|:...|..|.:       ...||.|    .|:...:....||:|||         :|...|.:
plant   587 -QTMSDPPSRNEQ-------LPPCKKL----RLDNNLLQSNGKEKVAS---------SKSKSSSS 630

  Fly   692 ATVNKADKSKDRTYKTECPLEFYDALPRV------GEICYAYEIFLEPQFE-SCEYTEHMYLNLQ 749
            |.   ..|.:...:.|.|.    :||...      |....||      :|: .|..:..:|
plant   631 AA---GSKKRKELHGTTCA----NALSGTWGENIDGATFQAY------KFDFCCNISGEVY---- 678

  Fly   750 TPRNYAILLRNKLPR---LAEMPLFSNQGKLHVRVANAPLEVIIQNSEQL---ELLHQF-----H 803
              .::::||.:.|..   ..||.|:..: || |:.:.:|...|..:.|:|   :...||     .
plant   679 --SSFSLLLESTLAEDVGKVEMDLYLVR-KL-VKASVSPCGQIRLSQEELVKAKYFQQFFFNGMF 739

  Fly   804 GMVF---------------RDILKIWHPFFVLDRRSKENSYLVVPLILG-AGEQKCFDWELMTN- 851
            |.:|               .|...:|||.|:         :|::|:... .......||..:.:
plant   740 GKLFVGSKSQGTKREFLLQTDTSSLWHPAFM---------FLLLPVETNDLASSATIDWSAINSC 795

  Fly   852 -----FRR------LPQSHGSNVQQREQQPAPRPEDF-EGKIVTQWYANYDK----PMLVTKVH- 899
                 |.:      |..|.|:.......|.....:.. |..::....|:.||    .::|..:|
plant   796 ASIVEFLKKNSLLDLRDSDGNQCNTSSGQEVLLDDKMEETNLIHFANASSDKNSLEELVVIAIHT 860

  Fly   900 -------RELTPLSYMEKNQQD-----KTYYEFTMSKYGNRIGDVVHKDKFMIEVR--------- 943
                   ..::..|.|...:.|     .||.|:...|||..:   .|.::.:::::
plant   861 GRIYSIVEAVSDSSAMSPFEVDASSGYATYAEYFNKKYGIVL---AHPNQPLMKLKQSHHAHNLL 922

  Fly   944 -DLTEQLTFYVHNRGKFNAKSKAKMKVILIPELCFNFNFPGDLWLKLIFLPSILNRMYFLLHAEA 1007
             |..|::......:.....|.|..:...|.|||....:.|..:...:..|||:::|:..|:.|..
plant   923 VDFNEEMVVKTEPKAGNVRKRKPNIHAHLPPELLARIDVPRAVLKSIYLLPSVMHRLESLMLASQ 987

  Fly  1008 LRKRFNTYLNLHLLPFNGTDYMPRPLEIDYSLKRNVDPLGNVIPTEDIEEPKSLLEPMPTKSIEA 1072
            ||:                       |||.|:.
plant   988 LRE-----------------------EIDCSID-------------------------------- 997

  Fly  1073 SVANLEITEFENPWQKYMEPVDLSRNLLSTYPVELDYYYHFSVGNVCEMNEMDFEDKEYWAKNQF 1137
                                                   :||:.:.
plant   998 ---------------------------------------NFSISST------------------- 1004

  Fly  1138 HMPTGNIYGNRTPAKTNANVPALMPSKPTVRGKVKPLLILQKTVSKEHITPAEQGEFLAAITASS 1202
                                                                   ..|.|:|..:
plant  1005 -------------------------------------------------------SILEAVTTLT 1014

  Fly  1203 AADVFDMERLEILGDSFLKLSATLYLASKYSDWNEGTLTEVKSKLVSNRNLLFCLIDADIPKTLN 1267
            ..:.|.|||||:||||.||..|:.:|..||.|.:||.|:..:..::||.||........:...:.
plant  1015 CPESFSMERLELLGDSVLKYVASCHLFLKYPDKDEGQLSRQRQSIISNSNLHRLTTSRKLQGYIR 1079

  Fly  1268 TIQFTPRYTWLPPG----ISLPHNVLALWRENPEFAKIIGPHNLRDLALGDEESLVK-GNCSDIN 1327
            ...|.|| .|..||    ..:|.......||.|...|..           .|...:| |...|:.
plant  1080 NGAFEPR-RWTAPGQFSLFPVPCKCGIDTREVPLDPKFF-----------TENMTIKIGKSCDMG 1132

  Fly  1328 YNRFVEGCRANGQSFYAGADFSSEVNFCVGLVTIPNKVIADTLEALLGVIVKNYGLQHAFKMLEY 1392
            :...|                              :|.::|..|||:|....:.||..:..|:::
plant  1133 HRWVV------------------------------SKSVSDCAEALIGAYYVSGGLSASLHMMKW 1167

  Fly  1393 FKICRADIDKPLTQLLNLELGGKKMRANVNTTEIDGFLINHYY--------LEKNLGYTFKDRRY 1449
            ..|   |:|               ...|:....|:...:..|.        ||:.:.:.|..:..
plant  1168 LGI---DVD---------------FDPNLVVEAINRVSLRCYIPKEDELIELERKIQHEFSAKFL 1214

  Fly  1450 LLQALTHPSYPTNRITGSYQELEFIGDAILDFLISAYIFENNTKMNPGALTDLRSALVNNTTLAC 1514
            |.:|:||.|.   |.:.||:.|||:||::|||||:.::|....:..||.:||||||.|||...|.
plant  1215 LKEAITHSSL---RESYSYERLEFLGDSVLDFLITRHLFNTYEQTGPGEMTDLRSACVNNENFAQ 1276

  Fly  1515 ICVRHRLHFFILAENAKLSEIISKFVNFQESQGHRVTNYVRILLEEADVQPTPLDLDDELDMTEL 1579
            :.|::.||..           :.:.....|:|                       ::|.|...:.
plant  1277 VAVKNNLHTH-----------LQRCATVLETQ-----------------------INDYLMSFQK 1307

  Fly  1580 PHANKCISQEAEKGVPPKGEFNMSTNVDVPKALGDVLEALIAAVYLDCR-DLQRTWEVIFNLFEP 1643
            |       .|..:.:|         ::..|||||||:|::..|:.:|.| ||.:.|.|    |||
plant  1308 P-------DETGRSIP---------SIQGPKALGDVVESIAGALLIDTRLDLDQVWRV----FEP 1352

  Fly  1644 ELQEFTR--KVPINHIRQLVEHKHAKPVFSSPIVEGETVMVSCQFTCMEKTIKVYGFGSNKDQAK 1706
            .|.....  |:.:...|:|.|...:...|.......:.|............:.:.|.||.:.. |
plant  1353 LLSPLVTPDKLQLPPYRELNELCDSLGYFFRVKCSNDGVKAQATIQLQLDDVLLTGDGSEQTN-K 1416

  Fly  1707 LS---AAKHALQQLSK 1719
            |:   ||.|.|.||.|
plant  1417 LALGKAASHLLTQLEK 1432

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Dcr-2	NP_523778.2	MPH1	6..542	CDD:224036	145/556 (26%)
		DEXDc	22..173	CDD:238005	57/153 (37%)
		Dicer_PBD	241..343	CDD:277191	17/114 (15%)
		HELICc	<438..495	CDD:197757	18/56 (32%)
		Dicer_dimer	572..665	CDD:281376	8/92 (9%)
		PAZ	843..1004	CDD:198017	37/200 (19%)
		RIBOc	1194..1391	CDD:238333	52/201 (26%)
		Rnc	1433..1719	CDD:223644	81/299 (27%)
		RIBOc	1448..1649	CDD:238333	60/201 (30%)
		dsrm	1653..1717	CDD:278464	14/66 (21%)
DCL3	NP_001154662.2	DEXDc	58..203	CDD:238005	56/151 (37%)
		Helicase_C	392..506	CDD:278689	37/127 (29%)
		PAZ_CAF_like	824..962	CDD:239210	25/140 (18%)
		RIBOc	1006..1175	CDD:238333	55/228 (24%)
		Rnc	1200..1435	CDD:223644	81/291 (28%)
		RIBOc	1214..1361	CDD:238333	60/203 (30%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0571
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG57860
OrthoDB	1	1.010	-	-		D1337630at2759
OrthoFinder	1	1.000	-	-		FOG0002051
OrthoInspector	1	1.000	-	-		mtm1149
orthoMCL	1	0.900	-	-		OOG6_101152
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	1	1.000	-	-		X1728
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	10	9.730