Protein Alignment Dcr-2 and Dcr-1

DIOPT Version :10

• Sequence 1: NP_523778.2
• Gene: Dcr-2 / 36993
• FlyBaseID: FBgn0034246
• Length: 1722
• Species: Drosophila melanogaster
• Sequence 2: XP_003436256.1
• Gene: Dcr-1 / 1273124
• VectorBaseID: AGAMI1_013337
• Length: 2336
• Species: Anopheles gambiae
• Alignment Length: 2428
• Identity: 545/2428 - (22%)
• Similarity: 865/2428 - (35%)
• Gaps: 831/2428 - (34%)

  Fly     6 IKPRGYQLRLVDHLTKSNGIVYLPTGSGKTFVAILVLK-----RFSQDFDKPIESGGKRALFMCN 65
            :.||.||..|:....:.|.||.:...|.|.|:|:.:::     |:|...:.|    ||
Mosquito    16 LTPRDYQTELLATAREENLIVCIAHNSAKEFLAVKLIQSMRTNRWSSHPEAP----GK------- 69

  Fly    66 TVELARQQ----AMAVRRCTNFKVGFYVGEQGVDDWTRGMWSDE-----------IKKNQVL-VG 114
            .:.|.|..    :..|...|:.:|.      .|||......|.|           :....|| .|
Mosquito    70 AIYLTRMDRSLLSSMVSNLTDLQVA------NVDDVEDSEGSHEPDGASNTPVTDVASADVLFFG 128

  Fly   115 TAQVFLDMVTQTYVALSSLSVVIIDECHHGTGHHPFREF-MRLFTIANQTKLP----RVVGLTGV 174
            :....|..:.|..|.:..:|::|:||||...|.....|. .||...|..:..|    |::||.|.
Mosquito   129 SETTLLQYIEQGTVRVQDISLLIVDECHKNYGRQELWEICARLTHQAPSSDRPAQRTRILGLAGP 193

  Fly   175 LIKGNEIT--NVATKLKELEITYRGNIITVSDTKEMENVMLYATKPTEVMVS-FPHQEQVLT--V 234
            | .|...|  .:..:|..||...|..|.|.||   :.:|:.::|||||:::. .|.:...||  :
Mosquito   194 L-HGAGCTPERLCWELHYLERCLRARIETASD---ITSVLRFSTKPTELILECIPPKPSNLTQLL 254

  Fly   235 TRLISAEI----EKFYVSL------------DLMNIGVQP--------IRRSKSLQCLRDPS--K 273
            ..||..:|    :..|..|            |..:...:|        :||  .|:.:.||:  .
Mosquito   255 RMLIQRQIAFLKQHRYEPLAVYGLDGNDSASDKPDTDGEPEKDEENDDLRR--ELKSIPDPTVGP 317

  Fly   274 KSFVKQLFNDFLYQMKEYGIYAASIAIISLIVEFD---------------------IKRRQAETL 317
            .|::||    :|..:.|:|.:.|....:.|:...|                     :.:.:|...
Mosquito   318 LSYLKQ----YLELLDEFGPWGADRGALELLTTIDQEKVKVPYDRHFLLFCMVYTTLLQARATVA 378

  Fly   318 SVKLMHRTALTLCE-----KIRHLL---------------------------VQKLQDMTY---- 346
            ||...|.|.|...:     |:|.||                           |...|.:.|
Mosquito   379 SVFAQHDTELERIKRYSTPKVRRLLEVLAWFGEQRNRPKDRNQPATLHHHQQVHNQQRILYCFCR 443

  Fly   347 ------------------DDDDDNVNTEEVIMNFSTPKVQRFLMSLKV----------------- 376
                              .|.|:.:...:.::.....:.:|..:..||
Mosquito   444 NVECKELEKSYHTFGAQIADVDERIKQLDGLLASVRKRTERLNLKHKVVDNAAAEGGGTLGVQSP 508

  Fly   377 --------------SFA--------DKDPKD-ICCLVFVERRYTCKCIYGLLLNYIQSTPELRNV 418
                          .||        ..|..| :|.|:|...|...:.:|.||....:|..|. ..
Mosquito   509 RHGHESRANNFRRKRFAGGGHSHHRSNDTTDALCGLIFCNNRAMARILYVLLYEVSRSQREF-EF 572

  Fly   419 LTPQFMVGRNNISPD---FESVLERKWQKSAIQQFRDGNANLMICSSVLEEGIDVQACNHVFILD 480
            ::||:.|.:...:|.   .::.:|.:.|:..:::||....||:|.:|||||||::..||.|...:
Mosquito   573 ISPQYTVDKVATNPQNCLKQTTIEHRKQEEVLKRFRMHECNLLIGTSVLEEGIELPKCNLVIRWN 637

  Fly   481 PVKTFNMYVQSKGRARTTEAKFVLFTA-------------------------------------- 507
            ....:..|.|.||||:...|..|||..
Mosquito   638 SPANYRSYAQCKGRAKAPGAYHVLFVTPENAASRNEQQEDMASIEDVSLDGMIPEGPKDDNTRET 702

  Fly   508 --DKER-------EKTIQQIYQYRKAHNDIAEYLKDRVLEKTEPELYEIK-----GHFQDDIDPF 558
              |::|       :..|:|:..||:     .|.|........||..:|:|     .|..:...|
Mosquito   703 IDDQDRRMIETATDAMIEQVAIYRE-----VEKLLLAKCRNGEPPDWELKHADCFNHCLEVYRP- 761

  Fly   559 TNENGAVLLPN----------NALAILHRYCQTIPTDAFGFVIP-WFHVLQEDERDRIFGVSAKG 612
             :..|||.|.:          ||:..|::||..:|:|.|..:.| |          |......||
Mosquito   762 -SSGGAVALQSNGTCASLWLGNAIQTLNKYCAKLPSDTFTKLTPIW----------RCATTVRKG 815

  Fly   613 K--HVISINMPVNCMLRDTIYSDPMDNVKTAKISAAFKACKVLYSLGELNERFVPKTLKERVASI 675
            :  :..:|.:|:|...::.|...||.....|:..||:..|::|::.|||:..|.|...:...|..
Mosquito   816 RKLYQYTIRLPINSPWKEDILGLPMPTETLARRLAAYMTCRMLHAAGELDNSFQPFGKEAFRAFE 880

  Fly   676 ADVHFEHWNKY----GDSVTATVNK-----ADKSKDRTYKTECPLEFYDALPRVGEICYAYEIFL 731
            ||     |..:    .|:...:.|.     ..|.:...||....: |.:..|....:.|.|.|.:
Mosquito   881 AD-----WENFELEESDAKILSENSDPRPGTTKRRQYYYKRIASV-FNECRPDAETVAYLYHIRM 939

  Fly   732 E-----PQFESCEYTEHMYLNLQTPRNYAILLRNKLPRLAEMPLFSNQGKLHVRVANAPLEVIIQ 791
            |     |: |.......:|...::.:.:.||....:|:::..|:|:..|::.|.:...|..|.:
Mosquito   940 ELICPIPE-EQNTRGRKIYAPEESAQGFGILTTKLIPKISSFPIFTRSGEVKVSLDLCPQRVKL- 1002

  Fly   792 NSEQLELLHQFHGMVFRDILKIWHPFFVLDRRSKENSYLVVPLIL-----GAGEQK--------C 843
            ::.|||:::.|....|..:|::.....:.|..:.||.:.:||.:.     .||:.:        .
Mosquito  1003 SAHQLEMVNCFVKYTFTKVLRLQKSLMLYDANATENCFFIVPTVKQAVAGAAGKDEPPLQSDDVM 1067

  Fly   844 FDWELMTNFRRLPQSHGSNVQQREQQPAPRPED-----------FEGKIVTQWYANYDKP--MLV 895
            .|||.:       :...:||.:  ..|...|::           |...:|..||.|.|:|  ..|
Mosquito  1068 VDWEFV-------EKIATNVHR--SGPTFIPDEARKGYTFDVGKFRDAVVMPWYRNRDQPQYFYV 1123

  Fly   896 TKVHRELTPLSYMEKNQQDKTYYEFTMSKYGNRIGDVVHKDKFMIEVRDLTEQLTF----YVHNR 956
            .::...|:|.|....:.. .|:.|:...||...|   .::.:.:::|...:.:|.|    ||:.:
Mosquito  1124 AEICNHLSPKSTFPGSNY-ATFEEYYHRKYKIHI---QNQRQPLLDVDHTSARLNFLTPRYVNRK 1184

  Fly   957 G----------KFNAKSKAKMKVILIPELCFNFNFPGDLWLKLIFLPSILNRMYFLLHAEALRKR 1011
            |          |...:...:.|.||:||||....||..||...:.||.:|.|:..||.|:.:|::
Mosquito  1185 GVALPTSSEETKRAKRENLEQKQILVPELCTIHPFPASLWRAAVCLPCVLYRINALLLADEIRRQ 1249

  Fly  1012 FNTYLNL---------------HLLPF--NGTDYMPRPLE---------IDYS------------ 1038
            ....|.|               .:|.|  |..|.:.:..|         ||.|
Mosquito  1250 VARDLRLGWENVDELQEGQFQWPMLSFGWNLADVLRKTKEQKIAQAQEAIDASAPEVEDEVELDK 1314

  Fly  1039 ----------------LK----------------------RNVDPLGNVI--------------- 1050
                            ||                      :..|..|.::
Mosquito  1315 EAPNVRDAAEVDEEDGLKMENGVIAEVEKSQVDGEDDTGDKKTDSDGTLLEIGTWSNEMAVGVGT 1379

  Fly  1051 --------------PT------------------------ED-------IEEPKSLLEPMP---- 1066
                          |:                        ||       .|:||:.:||..
Mosquito  1380 DNDMGEEGRRGASSPSFLRYDSDCSSNSSANFYSSDEYDEEDDYYLYDGSEKPKNAIEPSQEAVS 1444

  Fly  1067 -------------------TKSIEASV---ANLEITEF--ENPWQKYMEPVDLSRNLLSTYPVEL 1107
                               |:|.:..|   ..|:| ||  |...:......||.|.......:|.
Mosquito  1445 GTDNANDSGDKPGSRNRTITQSQDTVVNLGGRLKI-EFKSETDAEAIDSECDLQRQRTQQSIIER 1508

  Fly  1108 ----DYYYHFS---VGNVC------------EMNEMDFEDKEYWAKNQFHMPTGNIYGNRTPAKT 1153
                |..|..|   |...|            |..|..||.::...|:.... .|::.....|...
Mosquito  1509 SRQNDMLYQSSKNAVDGFCYSPSDRQCAEEREQAEQRFERQKNHTKDTIRQ-QGSLVRWNEPLSV 1572

  Fly  1154 N-----------ANVPALM---------------------------------------------- 1161
            :           |.:.|||
Mosquito  1573 SHWRSKLDESEAATLGALMDDLGGQPFTELVPYVEPEQLFELLVRNGSSGERWLRLHDLYCLNRP 1637

  Fly  1162 --PSKPTV------------------RGKVKPLLILQKTV-----------------SKEHITPA 1189
              |.:.|:                  .|...|..:::.|:                 .|:|...:
Mosquito  1638 FFPERYTIFRGGSQFDNFLDESCQEGEGASSPPKVIELTICDPFPAIAAGQMACNYKPKQHSMRS 1702

  Fly  1190 EQGE----------------------------------------FLAAITASSAADVFDMERLEI 1214
            ::..                                        .|.|:|.|:|.|..::||||.
Mosquito  1703 DEANKNGHDSAGVLSTTDEAGGVDYFSFDYQPDLSQHPGPSPAIILQALTMSNANDGINLERLET 1767

  Fly  1215 LGDSFLKLSATLYLASKYSDWNEGTLTEVKSKLVSNRNLL---------FCLIDADIPKTLNTIQ 1270
            :||||||.:.|.||..:|.:.:||.|:.::||.|||.||.         .|:|.|         :
Mosquito  1768 IGDSFLKYAITTYLYCRYDNVHEGKLSHLRSKQVSNLNLYRLGRRKRLGDCMIAA---------K 1823

  Fly  1271 FTPRYTWLPPGISLPHNVLALWRENPEFAKIIGPH-NLRDLALGDEESLVKGNCSDI-------- 1326
            |.|...||||...:|..:    .:....|||...| ||.||     ..:.:.:|::|
Mosquito  1824 FEPHDNWLPPCYYVPKEL----EQTLIDAKIPACHWNLADL-----PDIKRLSCAEICQLVKERA 1879

  Fly  1327 ------NYNRF-VEGCRANG------------------QSFYAGADFSSEVNFC---VGLVT--- 1360
                  :.:|| ::...:||                  .:...|.:..::...|   ..|||
Mosquito  1880 RAKRREDVDRFDLQHADSNGGEDGNEDDDDDDDDDEDDTTTGEGNEHEADNGSCYIPYNLVTQHS 1944

  Fly  1361 IPNKVIADTLEALLGVIVKNYGLQHAFKMLEYFKICRADIDKPLTQL------------------ 1407
            ||:|.:||.:|||:|..:...|.:.|...:.:..|....|.:|..:|
Mosquito  1945 IPDKSVADCVEALIGAYLIECGPRGALLFMAWLGIRVLPIREPPVKLNSNNETALTPYKATGQND 2009

  Fly  1408 ------------------------LNLELGGKKMRANVNTTEIDGFLINHYYLEKNLGYTFKDRR 1448
                                    .|:..||.:..|...:.|:...|......|:.|||.|:||.
Mosquito  2010 GPLSTGVTIAEYGHWVAPPSPMVRANITFGGIETGAAATSRELARLLQGFEEFEQALGYRFRDRS 2074

  Fly  1449 YLLQALTHPSYPTNRITGSYQELEFIGDAILDFLISAYIFENNTKMNPGALTDLRSALVNNTTLA 1513
            |||||:||.||..||:|..||.|||:||||||:||:.:::|:..:.:|||||||||||||||..|
Mosquito  2075 YLLQAMTHASYSPNRLTDCYQRLEFLGDAILDYLITRHLYEDRRQHSPGALTDLRSALVNNTIFA 2139

  Fly  1514 CICVRHRLHFFILAENAKLSEIISKFVNFQESQGHRVTNYVRILLEEADVQPTPLDLDDELDMTE 1578
            .:.|||..|.:.|..:..|.|:|.:||..|:..|||:|       ||....|   |.||||
Mosquito  2140 SLAVRHGFHKYFLHLSPGLQEVIDRFVRIQQENGHRIT-------EEEYYLP---DEDDEL---- 2190

  Fly  1579 LPHANKCISQEAEKGVPPKGE---FNMSTNVDVPKALGDVLEALIAAVYLDC-RDLQRTWEVIFN 1639
                    .:....|....||   ...:.:|:||||||||.|::..|::||. ..|...|:|...
Mosquito  2191 --------GEYGAMGEDGPGEGRGVGEAEDVEVPKALGDVFESIAGAIFLDSDMSLDTVWKVYRK 2247

  Fly  1640 LFEPELQEFTRKVPINHIRQLVEHKHAKPVFSSP--IVEGETVMVSCQFTCMEKTIKVY------ 1696
            :..||:::|:..||.:.||:|:|.:.....|..|  :.:|..|.|         |::|:
Mosquito  2248 MMGPEIEKFSSSVPKSPIRELLEMEPETAKFGKPEKLTDGRRVRV---------TVEVFGKGTFR 2303

  Fly  1697 GFGSNKDQAKLSAAKHALQQLSK 1719
            |.|.|...||.:|||.||:||.|
Mosquito  2304 GIGRNYRIAKCTAAKCALRQLKK 2326

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Dcr-2	NP_523778.2	DEXHc_dicer	6..204	CDD:350792	58/225 (26%)
		Dicer_PBD	241..343	CDD:277191	30/180 (17%)
		SF2_C_dicer	360..506	CDD:350189	46/188 (24%)
		Dicer_dimer	571..670	CDD:460900	28/101 (28%)
		PAZ	843..1004	CDD:198017	46/187 (25%)
		RIBOc	1194..1391	CDD:238333	70/245 (29%)
		RIBOc	1448..1649	CDD:238333	86/204 (42%)
		dsrm	1653..1717	CDD:425434	22/71 (31%)
Dcr-1	XP_003436256.1	DEAD-like_helicase_N	17..224	CDD:475120	58/224 (26%)
		Dicer_PBD	255..383	CDD:277191	24/133 (18%)
		P-loop containing Nucleoside Triphosphate Hydrolases	538..663	CDD:476819	40/125 (32%)
		Dicer_dimer	783..874	CDD:460900	28/100 (28%)
		PAZ_dicer_like	1062..1184	CDD:239209	28/134 (21%)
		RIBOc	1746..>1829	CDD:238333	35/91 (38%)
		RIBOc	2074..2257	CDD:238333	86/204 (42%)
		DSRM_DICER	2262..2325	CDD:380680	22/71 (31%)