Protein Alignment POSH and Sh3rf1

DIOPT Version :10

• Sequence 1: NP_523776.1
• Gene: POSH / 36990
• FlyBaseID: FBgn0040294
• Length: 838
• Species: Drosophila melanogaster
• Sequence 2: NP_067481.2
• Gene: Sh3rf1 / 59009
• MGIID: 1913066
• Length: 891
• Species: Mus musculus
• Alignment Length: 996
• Identity: 302/996 - (30%)
• Similarity: 435/996 - (43%)
• Gaps: 268/996 - (26%)

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            |||..|.|||||.|||||||.::||||||||||::||..||.|:::|||||||.||...:||||.
Mouse     1 MDESALLDLLECPVCLERLDASAKVLPCQHTFCKRCLLGIVGSRNELRCPECRTLVGSGVDELPS 65

  Fly    66 NVLLMRILEGMKQ---NAAAGKGEEKGEETETQPERAK------------------PQPPAESVA 109
            |:||:|:|:|:||   ....|.|   |..|.|...||:                  .||..::.:
Mouse    66 NILLVRLLDGIKQRPWKPGPGGG---GGTTCTNTLRAQGSTVVNCGSKDLQSSQCGQQPRVQAWS 127

  Fly   110 PPDNQLLQLQSHQQSHQPARHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHRIDNNWFV 174
            ||...:.|                   ||.|.||:::...|..||||.|||:|:::.::|.||:.
Mouse   128 PPVRGIPQ-------------------LPCAKALYNYEGKEPGDLKFSKGDIIILRRQVDENWYH 173

  Fly   175 GQANGQEGTFPINYVKVSVPLPM--PQCIAMYDFKMGPND-EEGCLEFKKSTVIQVMRRVDHNWA 236
            |:.:|..|.||.|:|::..|||.  |||.|:|||::...: ::.||.|.|..|:.|:||||.|||
Mouse   174 GEVSGVHGFFPTNFVQIIKPLPQPPPQCKALYDFEVKDKEADKDCLPFAKDDVLTVIRRVDENWA 238

  Fly   237 EGRIGQTIGIFPIAFVELNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVID----PTVVTES 297
            ||.:...||||||::||.|:|||:|::..                 .||||.:|    |:...:|
Mouse   239 EGMLADKIGIFPISYVEFNSAAKQLIEWD-----------------KPPVPGVDTAECPSATAQS 286

  Fly   298 SSGS---------------------SNSTPGSSNSSS--------TSSSN-------------NC 320
            :|.|                     :.|:.||.|..|        .||||             :|
Mouse   287 TSASKHPDTKKNTRKRHSFTSLTMANKSSQGSQNRHSMEISPPVLISSSNPTAAARISELSGLSC 351

  Fly   321 SPNHQISLPNT-------PQHVVASGSASVRFRDKGAKEKRHSLNALLGGGAPLSLLQTNRHSAE 378
            |...|:.:..|       |...|.:|.|.....|...:....|:|..|   .|..||     :|.
Mouse   352 SAPSQVHISTTGLIVTPPPSSPVTTGPAFTFPSDVPYQAALGSMNPPL---PPPPLL-----AAT 408

  Fly   379 IL-SLPHELSRLEVSSSTALKPTSAPQTSRVLKTTVQ-----QQMQPNLPWGYLALFPYKPRQTD 437
            :| |.|...:....:::.|......|:.  |:.::.|     .|.:|::   |:|::||.||:.|
Mouse   409 VLASTPSGATAAVAAAAAAAAAGMGPRP--VMGSSEQIAHLRPQTRPSV---YVAIYPYTPRKED 468

  Fly   438 ELELKKGCVYIVTERCVDGWFKGKNW-LDITGVFPGNYLTPL-RARDQQQLMHQWKYVPQNADAQ 500
            ||||:||.:::|.|||.|||:||.:. ....|||||||:.|: ||               ..:|.
Mouse   469 ELELRKGEMFLVFERCQDGWYKGTSMHTSKIGVFPGNYVAPVTRA---------------VTNAS 518

  Fly   501 MAQVQQHPVAPDVRLNNMLSMQPPDLPPRQQQATATTTSCSVWSKPVEALFSRKSEPKPETATAS 565
            .|:|.........|...|:|......|.::.|......:.||....|.:....::.|:.:.
Mouse   519 QAKVSMSTAGQASRGVTMVSPSTAGGPTQKPQGNGVAGNPSVVPTAVVSAAHIQTSPQAKV---- 579

  Fly   566 TTSSSSSGAVGLMRRLTHMKTRSKSPGASLQQVPKEAISTNVEFT-TNPSAKLHPVHVRSGSC-- 627
                           |.||.      |.......:.|:.|....: ..|:|.:.|:.|::.:|
Mouse   580 ---------------LLHMS------GQMTVNQARNAVRTVAAHSQERPTAAVTPIQVQNAACLG 623

  Fly   628 ------PSQLQHSQPLNET--PAAKTAA----------------------------------QQQ 650
                  |.....||||...  |||..||                                  :..
Mouse   624 PASVGLPHHSLASQPLPPMAGPAAHGAAVSISRTNAPMACAAGASLASPNMTSAVLETEPSGRTV 688

  Fly   651 QFL------PKQLPSASTNSVSYGSQRVKGSKERPHLICARQSLDAATFRSMYNNAASPP-PPTT 708
            ..|      |:...||..|| |.|.......||:..|:   :.|..|:.:.  ....||| .||.
Mouse   689 TILPGLPTSPESAASACGNS-SAGKPDKDSKKEKKGLL---KLLSGASTKR--KPRVSPPASPTL 747

  Fly   709 SV------APAVYAGGQQQVIPGGGAQSQLHANMIIAPS------------------HRKSHSLD 749
            .|      ||...|.|.:  :|.||:    |..:...|:                  |||:.|||
Mouse   748 DVELGAGEAPLQGAVGPE--LPLGGS----HGRVGSCPTDGDGPVAAGTAALAQDAFHRKTSSLD 806

  Fly   750 ASHVLSPSSNMITEAAIKASATTKSPYCTRESRFRCIVPYPPNSDIELELHLGDIIYVQRKQKNG 814
            ::..::|.......:........:...|   .|.|.:|.|||.|:.||||..|||::|.:|:::|
Mouse   807 SAVPIAPPPRQACSSLGPVMNEARPVVC---ERHRVVVSYPPQSEAELELKEGDIVFVHKKREDG 868

  Fly   815 WYKGTHARTHKTGLFPASFVE 835
            |:|||..|..||||||.||||
Mouse   869 WFKGTLQRNGKTGLFPGSFVE 889

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RAD18	6..>113	CDD:227719	60/127 (47%)
		RING-HC_SH3RF3	8..53	CDD:438408	32/44 (73%)
		SH3	139..191	CDD:473055	22/51 (43%)
		SH3_SH3RF_2	199..251	CDD:212721	27/52 (52%)
		SH3_SH3RF_3	424..477	CDD:212717	29/53 (55%)
		SH3_SH3RF_C	782..836	CDD:212719	32/54 (59%)
Sh3rf1	NP_067481.2	RING-HC_SH3RF1	8..55	CDD:438406	34/46 (74%)
		SH3	137..190	CDD:473055	23/52 (44%)
		SH3_SH3RF1_2	200..254	CDD:212863	27/53 (51%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	274..323		9/48 (19%)
		Interaction with RAC1. /evidence=ECO:0000250|UniProtKB:Q7Z6J0	292..362		12/69 (17%)
		Interaction with AKT2. /evidence=ECO:0000269|PubMed:14504284	446..549		41/120 (34%)
		SH3	455..509	CDD:473055	29/56 (52%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	531..554		5/22 (23%)
		PHA03247	<539..851	CDD:223021	70/351 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	687..750		18/68 (26%)
		SH3_SH3RF_C	836..890	CDD:212719	32/54 (59%)