DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment POSH and SH3RF3

DIOPT Version :9

Sequence 1:	NP_523776.1	Gene:	POSH / 36990	FlyBaseID:	FBgn0040294	Length:	838	Species:	Drosophila melanogaster
Sequence 2:	NP_001092759.1	Gene:	SH3RF3 / 344558	HGNCID:	24699	Length:	882	Species:	Homo sapiens

Alignment Length:	931	Identity:	311/931 - (33%)
Similarity:	450/931 - (48%)	Gaps:	192/931 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            |||.:|.|||||||||||||||:|||||||||||:||:.||.|:|:||||||||||.|.:||||.
Human    46 MDESSLLDLLECSVCLERLDTTAKVLPCQHTFCRRCLESIVCSRHELRCPECRILVGCGVDELPA 110

  Fly    66 NVLLMRILEGMKQNAAAGKGEEKGEETETQPERAKP-QP-PAESVAP-------------PDN-- 113
            |:||:|:|:|::|...||          |.|..:.| :| |.:|.||             |.:
Human   111 NILLVRLLDGIRQRPRAG----------TSPGGSPPARPIPGQSAAPTLAGGGGGAAGSTPGSPV 165

  Fly   114 -----------QLLQLQSHQQSHQPARHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHR 167
                       .|.:|.:.:.:  ||  .:...|||:..||:.:...|..||||.|||:|:::.:
Human   166 FLSAAAGSTAGSLRELATSRTA--PA--AKNPCLLPYGKALYSYEGKEPGDLKFNKGDIIVLRRK 226

  Fly   168 IDNNWFVGQANGQEGTFPINYVKVSVPLP--MPQCIAMYDFKMGPNDEE-GCLEFKKSTVIQVMR 229
            :|..|:.|:.:|.:|..|.:|::...|||  .||..|:|||:|...|:: .||.|.|..::.|:|
Human   227 VDEQWYHGELHGTQGFLPASYIQCIQPLPHAPPQGKALYDFEMKDKDQDKDCLTFTKDEILTVLR 291

  Fly   230 RVDHNWAEGRIGQTIGIFPIAFVELNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVIDPTVV 294
            |||.|||||.:|..|||||:.:||||.:||:|::.   ..| |.........:||
Human   292 RVDENWAEGMLGDKIGIFPLLYVELNDSAKQLIEM---DKP-CPAAASSCNASLP---------- 342

  Fly   295 TESSSGSSNS---TPGSSNSSSTSS-------SNNCSPNHQISLPNTPQHVVASGSASVRFRDKG 349
              |.||:..|   :|..|:|.:.|:       ..|....|..:            :.||..|...
Human   343 --SDSGAVASVAPSPTLSSSGAVSAFQRRVDGKKNTKKRHSFT------------ALSVTHRSSQ 393

  Fly   350 AKEKRHSLNALLGGGAPLSLLQTN-RHSAEILSLPHELS----RLEVSSSTALKPTSAPQTSRVL 409
            |...|||:..    .||:.:..:: |.:|.|..|.| ||    ..:||||....||:.|:.:.|
Human   394 AASHRHSMEI----SAPVLISSSDPRAAARIGDLAH-LSCAAPTQDVSSSAGSTPTAVPRAASV- 452

  Fly   410 KTTVQQQMQP--NLPWG-YLALFPYKPRQTDELELKKGCVYIVTERCVDGWFKGKNW-LDITGVF 470
              :.:|...|  .||.. ||||:.|||:::|||||.||.:|.|.|:|.||||||.:. ..::|||
Human   453 --SGEQGTPPKVQLPLNVYLALYAYKPQKSDELELHKGEMYRVLEKCQDGWFKGASLRTGVSGVF 515

  Fly   471 PGNYLTPLRARDQQQLMHQWKYVPQNAD-------------AQMAQVQQHPVAPDVRLNNMLSMQ 522
            ||||:||:            ..||....             |:......||.:..  |:::.:..
Human   516 PGNYVTPV------------SRVPAGGAGPPRNNVVGGSPLAKGITTTMHPGSGS--LSSLATAT 566

  Fly   523 PPDLPPRQQQATATTTSCSVWSKPVEALFSRKSEPKPETA--TASTTSSSSSGA----VGLMRRL 581
            .|.||....||.|...:.   |.|..:.....::|....|  |.||.:.|::.|    ...:..|
Human   567 RPALPITTPQAHAQHPTA---SPPTGSCLRHSAQPTASQARSTISTAAHSAAQAQDRPTATVSPL 628

  Fly   582 THMKTRSKSPGASL----------------QQVPKEAIS-TNVEFTTNP----SAKLH------P 619
            ....:.|:.|..||                |..|:.||. |:......|    :|.|:      |
Human   629 RTQNSPSRLPATSLRPHSVVSPQHSHQPPVQMCPRPAIPLTSAASAITPPNVSAANLNGEAGGGP 693

  Fly   620 VHVRSGSCPSQLQHSQPLNETPAAKTAAQQQQFLPKQLPSASTNSVSYGSQRVKGSKERPHLICA 684
            :.|.|.|.|:..  ...|:|..:.|  .:::..|.|.|..|||...|.....|..:.: |.:
Human   694 IGVLSTSSPTNT--GCKLDEKKSEK--KEKKSGLLKLLAGASTKKKSRSPPSVSPTHD-PQV--- 750

  Fly   685 RQSLDAATFRSMYNNAASPPPPTTSVAPAVYAGGQQQVIPGGGAQSQLHANMIIAPSHRKSHSLD 749
              ::||     :...|..|...:.|:      .|:....|   .:|::...|.:.|.|||:.|||
Human   751 --AVDA-----LLQGAVGPEVSSLSI------HGRAGSCP---IESEMQGAMGMEPLHRKAGSLD 799

  Fly   750 ASHVLSPSSNMITEAAIKASATTKSPYCTRESRFRCIVPYPPNSDIELELHLGDIIYVQRKQKNG 814
            .:.. |||    .:|.:..:|....|......|:|.:|.|||.|:.|:||..|||::|.:|:::|
Human   800 LNFT-SPS----RQAPLSMAAIRPEPKLLPRERYRVVVSYPPQSEAEIELKEGDIVFVHKKREDG 859

  Fly   815 WYKGTHARTHKTGLFPASFVE 835
            |||||..|..:|||||.||||
Human   860 WYKGTLQRNGRTGLFPGSFVE 880

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RING	11..53	CDD:238093	34/41 (83%)
		SH3	139..191	CDD:302595	18/51 (35%)
		SH3_SH3RF_2	199..251	CDD:212721	27/52 (52%)
		SH3_SH3RF_3	424..477	CDD:212717	31/54 (57%)
		SH3_SH3RF_C	782..836	CDD:212719	31/54 (57%)
SH3RF3	NP_001092759.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	18..42
		RING	56..98	CDD:238093	34/41 (83%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	124..159		10/44 (23%)
		SH3_SH3RF3_1	197..250	CDD:212861	19/52 (37%)
		SH3_SH3RF3_2	260..314	CDD:212864	27/53 (51%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	368..399		6/42 (14%)
		Interaction with RAC1. /evidence=ECO:0000269\|PubMed:20696164	369..439		21/86 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	433..458		9/27 (33%)
		SH3_SH3RF3_3	467..523	CDD:212858	32/55 (58%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	575..664		19/91 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	693..747		16/57 (28%)
		SH3_SH3RF_C	827..881	CDD:212719	31/54 (57%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	86	1.000	Domainoid score	I8151
eggNOG	1	0.900	-	-		E33208_3BCIE
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	382	1.000	Inparanoid score	I2058
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG47017
OrthoDB	1	1.010	-	-		D151303at33208
OrthoFinder	1	1.000	-	-		FOG0003366
OrthoInspector	1	1.000	-	-		otm42279
orthoMCL	1	0.900	-	-		OOG6_106609
Panther	1	1.100	-	-	LDO	PTHR14167
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R10491
SonicParanoid	1	1.000	-	-		X2254
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	14	13.870