DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment POSH and Sh3rf2

DIOPT Version :9

Sequence 1:	NP_523776.1	Gene:	POSH / 36990	FlyBaseID:	FBgn0040294	Length:	838	Species:	Drosophila melanogaster
Sequence 2:	NP_001029359.1	Gene:	Sh3rf2 / 307472	RGDID:	1308415	Length:	735	Species:	Rattus norvegicus

Alignment Length:	846	Identity:	228/846 - (26%)
Similarity:	345/846 - (40%)	Gaps:	242/846 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            ||:.||.|||||.||.|:||.|:||||||||||:.|||.|..:..:|||||||.||.|.|:.||.
  Rat     1 MDDLTLLDLLECPVCFEKLDVTAKVLPCQHTFCKPCLQRIFKAHKELRCPECRTLVFCSIEALPA 65

  Fly    66 NVLLMRILEGMK--QNAAAGKGEEKGEETETQPER-AKPQPPAESVAPPDNQLLQLQSHQQSHQP 127
            |:||:|:|:|::  .|:..|....:......|..| ||..|.:...:|     .:|....:.|..
  Rat    66 NLLLVRLLDGVRSGHNSWRGGSFRRPRILTLQDNRKAKSSPRSLQASP-----FRLGPTVRIHMD 125

  Fly   128 ARHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHRIDNNWFVGQANGQEGTFPINYVKV- 191
            .        :|.|.||.::......||||.|||:||::.::|.||:.|:.||..|.||.:.|:|
  Rat   126 G--------VPRAKALCNYRGKNPGDLKFNKGDVILLQRQLDENWYQGEINGVSGFFPASSVEVI 182

  Fly   192 -SVPLPMPQCIAMYDFKMGPNDE---EGCLEFKKSTVIQVMRRVDHNWAEGRIGQTIGIFPIAFV 252
             .:|.|.|.|.|:|:|.:...|:   :.||.|.|..||.|:.|||.|||||::|..:|||||.||
  Rat   183 KQLPQPPPLCRALYNFDLRDKDKSENQDCLTFLKDDVITVISRVDENWAEGKLGDKVGIFPILFV 247

  Fly   253 ELNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVIDPTVVTESSSGSSNSTPGSSNSSSTSSS 317
            |.|.:|:.||:.                                                  |..
  Rat   248 EPNLSARHLLEK--------------------------------------------------SKG 262

  Fly   318 NNCSPNHQISLPNTPQHVVASGSASVRFRDKGAKEKRHSLNALLGGG--APLSLLQT-NR--HSA 377
            :..|....:||.::|....|:.::::| :..|::.|        |.|  |..:.|.| ||  ||.
  Rat   263 HQLSRTKHLSLMSSPSRGKATNTSTLR-KSPGSRRK--------GSGQFAMTTALNTLNRMVHSP 318

  Fly   378 EILSLPHELSRLEVSSSTALKPTS--------------------------AP-----QTSRVLKT 411
            |    .|::  :|:|:...:..||                          ||     .|:.|...
  Rat   319 E----GHQM--VEISTPVLISSTSPSMLTQHGDRADFPASSAGQVSTSHPAPASPGHSTAMVSVP 377

  Fly   412 TVQQQMQPNLPWGYLALFPYKPRQTDELELKKGCVYIVTERCVDGWFKGKNWL-DITGVFPGNYL 475
            :.||.:..|:   ::||..|..:..:||:||||....|..:..|||.:|.:.: ..||:||.:|:
  Rat   378 SSQQHLSTNM---FVALHTYSAQGPEELDLKKGEGIRVLGKNQDGWLRGVSLVTGRTGIFPSDYV 439

  Fly   476 TPL---RARDQQQLMHQWKYVPQNADAQMAQVQQHPVAPDVRLNNMLSMQPPDLPPRQQQATATT 537
            .|:   .||.....       |.:         :||
  Rat   440 IPVFSSTARKTSSF-------PDS---------RHP----------------------------- 459

  Fly   538 TSCSVWSKPVEALFSRKS----EPKPE---------TATASTTSSSSSGAVGLMRRLTHMKTRSK 589
            |.|:.|:....::.|:.|    :|:..         ||.....|:|..|..| ...|..:::..:
  Rat   460 TVCTTWALSTSSVSSQGSFSEGDPRQSGPFRSVFVPTAVNPPRSTSGPGTSG-QGSLRKVRSSMR 523

  Fly   590 SPGASLQQVPKEAISTNVEFTTNPSAKLHPVHVRSGSCPSQLQHSQPLNETPA---------AKT 645
            ..| |||:..:..|.|   |... |.:..|..|..   |.:.|..||...||:         :|:
  Rat   524 KNG-SLQRPVQSGIPT---FMVG-SLRCSPAMVIR---PQKFQFYQPQGMTPSPTPIMVEIGSKS 580

  Fly   646 AAQQQQFLPKQLPSASTNSVSYGSQRVKGSKER---------------PHLIC----ARQSLDAA 691
            .:..:..|........|.:.|.|:..:...||.               |.::.    ::..::..
  Rat   581 ISTGEPALTCINRGGKTRTHSAGNSIIMEGKETPIKSEPPPKPPASAPPSILVKPENSKNGIEKQ 645

  Fly   692 TFRSMYNNAASPPPPTTSVA---------PAVYAGGQQQVIPGGGAQSQLHANMIIAPSHRKS-H 746
            .....:.| .||||...|.:         ||...|.|.:.:...|       .|.|..:||.. |
  Rat   646 VKTVRFQN-YSPPPTKHSASGPTSGKHEQPATLKGSQPEAVSSEG-------EMTILFAHRSGCH 702

  Fly   747 S 747
            |
  Rat   703 S 703

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RING	11..53	CDD:238093	27/41 (66%)
		SH3	139..191	CDD:302595	22/51 (43%)
		SH3_SH3RF_2	199..251	CDD:212721	26/54 (48%)
		SH3_SH3RF_3	424..477	CDD:212717	19/53 (36%)
		SH3_SH3RF_C	782..836	CDD:212719
Sh3rf2	NP_001029359.1	RING-HC_SH3RF2	11..55	CDD:319663	29/43 (67%)
		SH3_SH3RF2_1	128..181	CDD:212862	23/52 (44%)
		SH3_SH3RF2_2	191..247	CDD:212865	26/55 (47%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	260..301		10/49 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	335..373		5/37 (14%)
		Interaction with PAK4. /evidence=ECO:0000250\|UniProtKB:Q8TEC5	373..466		31/140 (22%)
		SH3_SH3RF2_3	387..441	CDD:212718	19/56 (34%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	472..534		14/63 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	612..637		2/24 (8%)
		Interaction with PPP1CA. /evidence=ECO:0000250	647..652		0/4 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	649..735		17/63 (27%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D151303at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR14167
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	5	4.980