DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment POSH and Sh3rf2

DIOPT Version :9

Sequence 1:	NP_523776.1	Gene:	POSH / 36990	FlyBaseID:	FBgn0040294	Length:	838	Species:	Drosophila melanogaster
Sequence 2:	NP_001139771.1	Gene:	Sh3rf2 / 269016	MGIID:	2444628	Length:	735	Species:	Mus musculus

Alignment Length:	839	Identity:	234/839 - (27%)
Similarity:	341/839 - (40%)	Gaps:	228/839 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            ||:.||.|||||.||.|:||.|:||||||||||:.|||.|..:..:|||||||.||.|.|:.||.
Mouse     1 MDDLTLLDLLECPVCFEKLDVTAKVLPCQHTFCKPCLQRIFKAHKELRCPECRTLVFCSIEALPA 65

  Fly    66 NVLLMRILEGMKQNAAAGKGE--EKGEETETQPERAKPQPPAESVAPPDNQLLQLQSHQQSHQPA 128
            |:||:|:|:|::...::.||.  .:......|..|.....|....|.|...:..::.|...
Mouse    66 NLLLVRLLDGVRSGQSSWKGGSFRRPRILTLQDNRKAKSSPRSLQASPFRLVPSVRIHMDG---- 126

  Fly   129 RHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHRIDNNWFVGQANGQEGTFPINYVKV-- 191
                    :|.|.||.::......||||.|||:||::.::|.||:.|:.||..|.||.:.|:|
Mouse   127 --------VPRAKALCNYRGKNPGDLKFNKGDVILLRRQLDENWYQGEINGVSGIFPASSVEVIK 183

  Fly   192 SVPLPMPQCIAMYDFKMGPNDE---EGCLEFKKSTVIQVMRRVDHNWAEGRIGQTIGIFPIAFVE 253
            .:|.|.|.|.|:|:|.:...|:   :.||.|.|..||.|:.|||.|||||::|..:|||||.|||
Mouse   184 QLPQPPPLCRALYNFDLRDKDKSENQDCLTFLKDDVITVISRVDENWAEGKLGDKVGIFPILFVE 248

  Fly   254 LNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVIDPTVVTESSSGSSNSTPGSSNSSS----- 313
            .|.:|:.||::..|          |..|......:..|:....:::.|...:|||....|
Mouse   249 PNVSARHLLENKGH----------QLSRTRHLSLMSSPSRGKATNTSSLRKSPGSRRKGSGQFSM 303

  Fly   314 TSSSNNC-----SP-NHQISLPNTPQHVVASGSASVRFRDKGAKEKRHSLNALLGGGAPLSLLQT 372
            |::.|..     || .||:...:||. :::|.|.|| ....|.|           ...|.|
Mouse   304 TTALNTLNRTVHSPEGHQMVEISTPM-LISSNSPSV-LTQHGDK-----------ADFPTS---- 351

  Fly   373 NRHSAEILSLPHELSRLEVSSSTALKPTSAPQTSRVLKTTVQQQMQPNLPWGYLALFPYKPRQTD 437
                          |..:||||.....:....|:.|...:.||.:..|:   ::||..|...:.:
Mouse   352 --------------STGQVSSSQPAPASPGHSTAMVSVPSSQQHLSNNM---FVALHTYSAHRPE 399

  Fly   438 ELELKKGCVYIVTERCVDGWFKGKNWL-DITGVFPGNYLTPL---RARDQQQLMHQWKYVPQNAD 498
            ||:|:||....|..:..|||.||.:.| ..||:||.:|:.|:   .||.....
Mouse   400 ELDLQKGEGIRVLGKYQDGWLKGLSLLTGRTGIFPSDYVIPVFSSTARKTSSF------------ 452

  Fly   499 AQMAQVQQHPVAPDVRLNNMLSMQPPDLPPRQQQATATTTSCSVWSKPVEALFSRKSEPKPETAT 563
                        ||.|                     :.|.|:.|:
Mouse   453 ------------PDSR---------------------SPTVCTTWA------------------- 465

  Fly   564 ASTTSSSSSGAVGLMRRLTHMKTRSKSPGASLQQVPKEAISTNVEFTTNPSA-------KLHPVH 621
            .||:|.||.|:      ......|...|..|: .||...::.: ..|..|.:       |:....
Mouse   466 LSTSSVSSQGS------FAEGDPRQSGPFKSV-FVPTAVVNPS-RSTPGPGSSGQGSLRKVRSSM 522

  Fly   622 VRSGSCPSQLQHSQP------LNETPAAKTAAQQQQFLPKQ--LPSASTNSVSYGSQRV------ 672
            .::||....:|...|      |..:|......|:.||.|.|  .||.:...|..||:.:
Mouse   523 RKNGSLQRPVQSGIPTFMVGSLRCSPTMVIRPQRFQFYPPQGMTPSPTPIMVEMGSKSIYTGEPA 587

  Fly   673 -----KGSKER------------------------------PHLICARQSLDAATFRSM----YN 698
                 :|||.|                              |.::...::....|.:.:    :.
Mouse   588 LTCINRGSKTRIHSAGNSIIMEGKETPIKSEPPPKPPASAPPSILVKPENSKNGTEKQVKTVRFQ 652

  Fly   699 NAASPPPPTTSVA---------PAVYAGGQQQVIPGGGAQSQLHANMIIAPSHRKS-HS 747
            | .||||...|.:         ||...|.|.:.:..||       .|.|..:||.. ||
Mouse   653 N-YSPPPTKHSASSPTSGKHDHPATLKGSQHEAVSSGG-------EMTILFAHRSGCHS 703

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RING	11..53	CDD:238093	27/41 (66%)
		SH3	139..191	CDD:302595	22/51 (43%)
		SH3_SH3RF_2	199..251	CDD:212721	26/54 (48%)
		SH3_SH3RF_3	424..477	CDD:212717	20/53 (38%)
		SH3_SH3RF_C	782..836	CDD:212719
Sh3rf2	NP_001139771.1	RING-HC_SH3RF2	11..55	CDD:319663	29/43 (67%)
		RING-HC finger (C3HC4-type)	12..52	CDD:319663	25/39 (64%)
		SH3_SH3RF2_1	128..181	CDD:212862	23/52 (44%)
		SH3_SH3RF2_2	191..247	CDD:212865	26/55 (47%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	262..302		9/49 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	333..378		15/74 (20%)
		Interaction with PAK4. /evidence=ECO:0000250\|UniProtKB:Q8TEC5	372..465		32/140 (23%)
		SH3_SH3RF2_3	386..440	CDD:212718	20/56 (36%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	497..534		5/37 (14%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	612..720		20/100 (20%)
		Interaction with PPP1CA. /evidence=ECO:0000250	647..652		0/4 (0%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D151303at33208
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm44333
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR14167
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	5	4.980