DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment POSH and Sh3rf3

DIOPT Version :9

Sequence 1:	NP_523776.1	Gene:	POSH / 36990	FlyBaseID:	FBgn0040294	Length:	838	Species:	Drosophila melanogaster
Sequence 2:	NP_766376.2	Gene:	Sh3rf3 / 237353	MGIID:	2444637	Length:	878	Species:	Mus musculus

Alignment Length:	925	Identity:	300/925 - (32%)
Similarity:	431/925 - (46%)	Gaps:	179/925 - (19%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MDEHTLNDLLECSVCLERLDTTSKVLPCQHTFCRKCLQDIVASQHKLRCPECRILVSCKIDELPP 65
            |||.:|.|||||||||||||||:|||||||||||:||:.||.|:|:||||||||||.|.:||||.
Mouse    41 MDESSLLDLLECSVCLERLDTTAKVLPCQHTFCRRCLESIVCSRHELRCPECRILVGCGVDELPA 105

  Fly    66 NVLLMRILEGMKQNAAAGKGEEKGEETETQPERAKP---------------QPPAESV------A 109
            |:||:|:|:|::|....|     .....:.|.|..|               .||...|      .
Mouse   106 NILLVRLLDGIRQRPRTG-----ASPGSSPPARPGPGTFSALAGGAGGATGSPPCSPVFLSAAAG 165

  Fly   110 PPDNQLLQLQSHQQSHQPARHKQRRFLLPHAYALFDFASGEATDLKFKKGDLILIKHRIDNNWFV 174
            ...:.|..:.:::.........|    ||:|.||:.:...|..||||.|||:|:::.::|.||:.
Mouse   166 SSTSSLCDVATNRSVPVAKTLSQ----LPYAKALYSYEGKEPGDLKFNKGDIIILRRKVDENWYH 226

  Fly   175 GQANGQEGTFPINYVKVSVPLP--MPQCIAMYDFKMGPNDEE-GCLEFKKSTVIQVMRRVDHNWA 236
            |:..|..|..|.:|::...|||  :||..|:|||:|...|:: .||.|.|..|:.|:||||.|||
Mouse   227 GELQGMHGFLPASYIQCVRPLPQALPQGKALYDFEMKDRDQDKDCLTFTKDEVLTVIRRVDDNWA 291

  Fly   237 EGRIGQTIGIFPIAFVELNAAAKKLLDSGLHTHPFCHPPKQQGQRALPPVPVIDPTVVTESSSGS 301
            ||.:|..|||||:.:||||.:||:|::    ....|  |..........:...||:.|...:.|.
Mouse   292 EGMLGDKIGIFPLLYVELNDSAKQLIE----MDKLC--PAATTAYNYDALLSSDPSTVASVAPGP 350

  Fly   302 SNSTPG--SSNSSSTSSSNNCSPNHQISLPNTPQHVVASGSASVRFRDKGAKEKRHSLNALLGGG 364
            :.|:.|  |:......|..|....|..:            :.||..:...|...|||:..    .
Mouse   351 TLSSSGAVSAFQRRVDSKKNAKKRHSFT------------ALSVTHKSSQAASHRHSMEI----S 399

  Fly   365 APLSLLQTN-RHSAEILSLPHELS---RLEVSSSTALKPTSAPQTSRVLKTTVQQQMQP--NLPW 423
            ||:.:..:: |.:|.|..|.| ||   ..:.|||....||:.|:.:.|   ..:|.|.|  .||.
Mouse   400 APVLISSSDPRAAARIGELAH-LSCTVPTQDSSSAGPVPTALPRAAAV---AGEQGMSPKVQLPL 460

  Fly   424 G-YLALFPYKPRQTDELELKKGCVYIVTERCVDGWFKGKNW-LDITGVFPGNYLTPLR------- 479
            . ||||:.|||::.|||||:||.:|.|.|:|.||||||.:. ..::|||||||:||:.
Mouse   461 NVYLALYAYKPQKNDELELRKGEMYRVLEKCQDGWFKGASLKTGVSGVFPGNYVTPVSRVPGGGA 525

  Fly   480 -------------ARDQQQLMHQWKYVPQNADAQMAQVQQHPVAPDVRLNNMLSMQPPDLP---- 527
                         |:....:||     |................|...|.:.:......||
Mouse   526 GLPWNNVLGGSPLAKGMATIMH-----PGGGSLSSPATAARSALPLTTLQDHMQHPATSLPTGSC 585

  Fly   528 ------PRQQQA------TATTTSCSVWSKPVEALFSRKSEPKPETATAS----TTSSSSSGAVG 576
                  |...||      |||..|.....:|              |||.|    .||.|...:.|
Mouse   586 LRHSAQPTASQAGDTTIPTATHASAQALDRP--------------TATVSPLRTQTSPSRLPSTG 636

  Fly   577 LM-RRLTHMKTRSKSPGASLQQVPKEAISTNVEFTTNPSAKLHPVHVRSGSCPSQLQHSQPLN-- 638
            |. |.:...:...:||.   |..|:.||    .||:..|| :.|.:|.:.:...:: ...|::
Mouse   637 LRPRSVASPQHGQQSPA---QMCPRPAI----PFTSAASA-ITPPNVSAANLSGEV-GGTPISGL 692

  Fly   639 ETPA-----------AKTAAQQQQFLPKQLPSASTNSVSYGSQRVKGSKERPHLICARQSLDAAT 692
            .||:           .....:::..|.|.|..|||...|.....|..:.:      .:.::|.:
Mouse   693 STPSLINTGFKPDDKKNEKKEKKSGLLKLLAGASTKKKSRSPPSVSPTHD------PQSAMDTS- 750

  Fly   693 FRSMYNNAASPPPPTTSVAPAVYAG--GQQQVIPGGGAQSQLHANMIIAPSHRKSHSLDASHVLS 755
                ...|..|     .|:|....|  |...:      :|::...:.:.|.|||:.|||.:..||
Mouse   751 ----LQGAMGP-----EVSPLTVHGRAGSCPI------ESEMQGAIGLEPLHRKAGSLDLNFSLS 800

  Fly   756 PSSNMITEAAIKASATTKSPYCTRESRFRCIVPYPPNSDIELELHLGDIIYVQRKQKNGWYKGTH 820
            ||    .:|.:..::....|......|:|.:|.|||.|:.|:||..|||::|.:|.::||:|||.
Mouse   801 PS----RQATLSMASIRPEPKPLPRERYRVVVSYPPQSEAEIELKEGDIVFVHKKHEDGWFKGTL 861

  Fly   821 ARTHKTGLFPASFVE 835
            .|..:|||||.||||
Mouse   862 QRNGRTGLFPGSFVE 876

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
POSH	NP_523776.1	RING	11..53	CDD:238093	34/41 (83%)
		SH3	139..191	CDD:302595	20/51 (39%)
		SH3_SH3RF_2	199..251	CDD:212721	28/52 (54%)
		SH3_SH3RF_3	424..477	CDD:212717	31/54 (57%)
		SH3_SH3RF_C	782..836	CDD:212719	30/54 (56%)
Sh3rf3	NP_766376.2	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	19..40
		RING	51..93	CDD:238093	34/41 (83%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	120..145		4/29 (14%)
		SH3	190..243	CDD:302595	21/52 (40%)
		SH3	253..307	CDD:302595	28/53 (53%)
		Interaction with RAC1. /evidence=ECO:0000250\|UniProtKB:Q8TEJ3	364..433		20/85 (24%)
		SH3_SH3RF3_3	461..517	CDD:212858	32/55 (58%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	574..659		24/101 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	688..758		14/85 (16%)
		SH3_SH3RF_C	823..877	CDD:212719	30/54 (56%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	86	1.000	Domainoid score	I8155
eggNOG	1	0.900	-	-		E33208_3BCIE
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	380	1.000	Inparanoid score	I2044
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG47017
OrthoDB	1	1.010	-	-		D151303at33208
OrthoFinder	1	1.000	-	-		FOG0003366
OrthoInspector	1	1.000	-	-		otm44333
orthoMCL	1	0.900	-	-		OOG6_106609
Panther	1	1.100	-	-	LDO	PTHR14167
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R10491
SonicParanoid	1	1.000	-	-		X2254
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	15	14.870