DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment eIF3b and PRT1

DIOPT Version :9

Sequence 1:	NP_611228.1	Gene:	eIF3b / 36981	FlyBaseID:	FBgn0034237	Length:	690	Species:	Drosophila melanogaster
Sequence 2:	NP_015006.3	Gene:	PRT1 / 854543	SGDID:	S000005888	Length:	763	Species:	Saccharomyces cerevisiae

Alignment Length:	739	Identity:	218/739 - (29%)
Similarity:	375/739 - (50%)	Gaps:	95/739 - (12%)

- Green bases have known domain annotations that are detailed below.

  Fly    21 EEPNFDDPPGFVDNISDEDLLGDMLAQRPSEADGVESVVVVDNIPKVEPVRLEKLKLVINKLFSN 85
            |:...:|.|  ||:|...||...   .:.:|....:..:||:..|.:...::..||..:..|||.
Yeast    46 EDIKLEDIP--VDDIDFSDLEEQ---YKVTEEFNFDQYIVVNGAPVIPSAKVPVLKKALTSLFSK 105

  Fly    86 YGEIVNVVYPVDE-EGKTKGYAFMEYKQARQAEEAVKKLNNHRLDKNHTFAVNLFTDFQKY--EN 147
            .|::||:.:|:|| .|||||:.|:|......|::.:|..:..|||..|...:....|.::|  ::
Yeast   106 AGKVVNMEFPIDEATGKTKGFLFVECGSMNDAKKIIKSFHGKRLDLKHRLFLYTMKDVERYNSDD 170

  Fly   148 IPEKWEPPTVQTFKVQSDLYNFINDPDTYDQYCVAAETAPNCVQVGFWQNVLPEPFEL-ETRERF 211
            ...::..|.:.||...|.|.:::.|....||:.:..:...:.    ||.::..|...| |:||.:
Yeast   171 FDTEFREPDMPTFVPSSSLKSWLMDDKVRDQFVLQDDVKTSV----FWNSMFNEEDSLVESRENW 231

  Fly   212 TDTFVKWSPLGTYVVTFHKPGVAIWGGSSFQKIQKFPHPGTQFVEFSPCENYLVTYGPTP----- 271
            :..:|::||.|||:.::|:.||..|||.:|.::::|.||..:....||.|.||||:...|
Yeast   232 STNYVRFSPKGTYLFSYHQQGVTAWGGPNFDRLRRFYHPDVRNSSVSPNEKYLVTFSTEPIIVEE 296

  Fly   272 -----------TGQKIIIWDIRTGAEKRSFVADGMSVLS--MFRWSHDDKFVARMGENSIHIYE- 322
                       .|.::.||||.:|....:|.......|.  :.|||::||:.|||..:|:.:::
Yeast   297 DNEFSPFTKKNEGHQLCIWDIASGLLMATFPVIKSPYLKWPLVRWSYNDKYCARMVGDSLIVHDA 361

  Fly   323 TPSFYLLDLKSIKIPGIRGFSWSP-------------TDNVIAYWVEEQNQIPARVTLMEIPKKR 374
            |.:|..|:.|::|..|||.||::|             ...::|||..|.|......|:.|:|:.|
Yeast   362 TKNFMPLEAKALKPSGIRDFSFAPEGVKLQPFRNGDEPSVLLAYWTPETNNSACTATIAEVPRGR 426

  Fly   375 EIRNKNLFHVADCKLHWQKSGDYLCVKVDRYSKLKKDKKDLDVKFLGMFYNFEIFHMREKEIPVD 439
            .::..||..|::..||||...::||..|:|::|..|.:          |.|.:|..:.|::|||:
Yeast   427 VLKTVNLVQVSNVTLHWQNQAEFLCFNVERHTKSGKTQ----------FSNLQICRLTERDIPVE 481

  Fly   440 SVEIRELILAFAWEPVGNKFSIIH----GETN----SSNVSFYEVNKGVKP------SLVKRLEK 490
            .||:::.:..|.|||.||:|..|.    .:.|    ::.:.||......|.      ||||.:.|
Yeast   482 KVELKDSVFEFGWEPHGNRFVTISVHEVADMNYAIPANTIRFYAPETKEKTDVIKRWSLVKEIPK 546

  Fly   491 KSCTHLFWSPRGQFIVMA-----NLTMGTFEFVD----------STNDYIITAS------PDHFR 534
            .....:.|||.|:|:|:.     |:.....:|.|          ..||  ::||      |.:..
Yeast   547 TFANTVSWSPAGRFVVVGALVGPNMRRSDLQFYDMDYPGEKNINDNND--VSASLKDVAHPTYSA 609

  Fly   535 ASEVEWDPTGRYVVTGVSSWKVKEDTGFNMYTFQGRIIKRTILKNFVQFLWRPRPPTLLSEEKQK 599
            |:.:.|||:||||....||.|.|.:.|:.::...|.::|..|:..|..|.|||||.::||..::|
Yeast   610 ATNITWDPSGRYVTAWSSSLKHKVEHGYKIFNIAGNLVKEDIIAGFKNFAWRPRPASILSNAERK 674

  Fly   600 EIKKNLKKYYAAFEQKDRLRLTRASKELLEKRSQLRETFMEYRNKRIAEWAEQKSRRIM-LRGHV 663
            :::|||:::.|.||::|.:....|.::|:..:.:|.:.:.|||.|...|..:..:.:|. ::...
Yeast   675 KVRKNLREWSAQFEEQDAMEADTAMRDLILHQRELLKQWTEYREKIGQEMEKSMNFKIFDVQPED 739

  Fly   664 DTDNLET-DEVDEEIVEFLVKEEV 686
            .:|:..| :|:.||::| ..||:|
Yeast   740 ASDDFTTIEEIVEEVLE-ETKEKV 762

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
eIF3b	NP_611228.1	WD40	<216..352	CDD:295369	52/167 (31%)
		WD40 repeat	216..248	CDD:293791	13/31 (42%)
		WD40 repeat	297..321	CDD:293791	10/25 (40%)
		RRM_eIF3B	56..137	CDD:240724	27/81 (33%)
		COG5354	194..690	CDD:227657	172/563 (31%)
PRT1	NP_015006.3	RRM_eIF3B	76..158	CDD:409720	27/81 (33%)
PRT1	NP_015006.3	COG5354	200..746	CDD:227657	166/561 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C157345431
Domainoid	1	1.000	128	1.000	Domainoid score	I1154
eggNOG	1	0.900	-	-		E1_COG5354
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H2780
Inparanoid	1	1.050	338	1.000	Inparanoid score	I415
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG54076
OrthoFinder	1	1.000	-	-		FOG0003562
OrthoInspector	1	1.000	-	-		oto99340
orthoMCL	1	0.900	-	-		OOG6_101924
Panther	1	1.100	-	-	LDO	PTHR14068
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R377
SonicParanoid	1	1.000	-	-		X2436
TreeFam	1	0.960	-	-
	15	14.790